| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Proteins1
Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany
T cell activation leads to the induction of the transcription factors of the NFAT and NF-
B families, important regulators of T cell activation and function. In this study we demonstrate that TCR/CD3-stimulated T cells from mice expressing a constitutively active form of protein kinase B (myr PKB
) lack significant nuclear accumulation/shuttling of NFATc1 and NFATp as well as NF-Bp65 and RelB proteins. Notably, despite this deficit in nuclear NFAT and NF-B proteins, myr PKB T cells show lower activation threshold for proliferation, enhanced cell cycle progression and increased production of Th1 and Th2 cytokines similar to signals provided by CD28 costimulation. The enhanced T cell response correlates with increased expression of cyclins D3 and B1 and cytokine-induced Src homology 2 protein, and inactivation of the forkhead transcription factor FKHR. In addition, coimmunoprecipitation studies indicate a direct regulation of NFATc1 by active PKB. Together, our results demonstrate that the positive regulatory role of myr PKB on TCR responsiveness, subsequent cell division, and effector function is linked to a negative regulatory mechanism on the nuclear accumulation/shuttling of NFAT and NF-B proteins.
This article has been cited by other articles:
|
|
 |
|
  A. Akimzhanov, L. Krenacs, T. Schlegel, S. Klein-Hessling, E. Bagdi, E. Stelkovics, E. Kondo, S. Chuvpilo, P. Wilke, A. Avots, et al. Epigenetic Changes and Suppression of the Nuclear Factor of Activated T Cell 1 (NFATC1) Promoter in Human Lymphomas with Defects in Immunoreceptor Signaling Am. J. Pathol., January 1, 2008; 172(1): 215 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. D. Schneider, A. A. Weiss, and W. E. Miller Pertussis Toxin Utilizes Proximal Components of the T-Cell Receptor Complex To Initiate Signal Transduction Events in T Cells Infect. Immun., August 1, 2007; 75(8): 4040 - 4049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jaworska, A. Gravel, K. Fink, N. Grandvaux, and L. Flamand Inhibition of Transcription of the Beta Interferon Gene by the Human Herpesvirus 6 Immediate-Early 1 Protein J. Virol., June 1, 2007; 81(11): 5737 - 5748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Kemp, L. Wille, C. L. Lewis, L. B. Nicholson, and D. A. Lauffenburger Quantitative Network Signal Combinations Downstream of TCR Activation Can Predict IL-2 Production Response J. Immunol., April 15, 2007; 178(8): 4984 - 4992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gururajan, T. Dasu, S. Shahidain, C. D. Jennings, D. A. Robertson, V. M. Rangnekar, and S. Bondada Spleen Tyrosine Kinase (Syk), a Novel Target of Curcumin, Is Required for B Lymphoma Growth J. Immunol., January 1, 2007; 178(1): 111 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Patra, T. Drewes, S. Engelmann, S. Chuvpilo, H. Kishi, T. Hunig, E. Serfling, and U. H. Bommhardt PKB Rescues Calcineurin/NFAT-Induced Arrest of Rag Expression and Pre-T Cell Differentiation J. Immunol., October 1, 2006; 177(7): 4567 - 4576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dejmek, A. Safholm, C. Kamp Nielsen, T. Andersson, and K. Leandersson Wnt-5a/Ca2+-Induced NFAT Activity Is Counteracted by Wnt-5a/Yes-Cdc42-Casein Kinase 1{alpha} Signaling in Human Mammary Epithelial Cells. Mol. Cell. Biol., August 1, 2006; 26(16): 6024 - 6036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kim and E. Y. Denkers Toxoplasma gondii triggers Gi-dependent PI 3-kinase signaling required for inhibition of host cell apoptosis J. Cell Sci., May 15, 2006; 119(10): 2119 - 2126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Trotta, R. Parihar, J. Yu, B. Becknell, J. Allard II, J. Wen, W. Ding, H. Mao, S. Tridandapani, W. E. Carson, et al. Differential expression of SHIP1 in CD56bright and CD56dim NK cells provides a molecular basis for distinct functional responses to monokine costimulation Blood, April 15, 2005; 105(8): 3011 - 3018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Hammerman, C. J. Fox, R. M. Cinalli, A. Xu, J. D. Wagner, T. Lindsten, and C. B. Thompson Lymphocyte Transformation by Pim-2 Is Dependent on Nuclear Factor-{kappa}B Activation Cancer Res., November 15, 2004; 64(22): 8341 - 8348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Bommhardt, K. C. Chang, P. E. Swanson, T. H. Wagner, K. W. Tinsley, I. E. Karl, and R. S. Hotchkiss Akt Decreases Lymphocyte Apoptosis and Improves Survival in Sepsis J. Immunol., June 15, 2004; 172(12): 7583 - 7591. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
