The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Proud, D.
Right arrow Articles by Wiehler, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Proud, D.
Right arrow Articles by Wiehler, S.
The Journal of Immunology, 2004, 172: 4637-4645.
Copyright © 2004 by The American Association of Immunologists

Human Rhinovirus Infection Induces Airway Epithelial Cell Production of Human {beta}-Defensin 2 Both In Vitro and In Vivo1

David Proud2,*, Scherer P. Sanders{dagger} and Shahina Wiehler*

* Respiratory Research Group and Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada; and {dagger} Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224

We hypothesized that airway epithelial cells, the primary site of human rhinovirus (HRV) infection, provide a link between the innate and specific immune response to HRV via production of human {beta}-defensin (HBD)-2, a potent in vitro attractant and activator of immature dendritic cells. Infection of primary cultures of human epithelial cells with several HRV serotypes induced expression of HBD-2 mRNA and protein, indicating that HBD-2 production was independent of viral receptor usage or mechanisms of viral RNA internalization. Induction of HBD-2 was dependent upon viral replication and could be mimicked by transfection of cells with synthetic dsRNA, but was not dependent upon epithelial production of IL-1. Studies with stable epithelial cell lines expressing HBD-2 promoter constructs, as well as inhibitor studies in primary cells, both demonstrated that induction of HBD-2 involves activation of the transcription factor, NF-{kappa}B. Other transcription factors must also be activated by HRV infection, however, as expression of HBD-3 mRNA was also induced and there is no putative NF-{kappa}B recognition sequence in the promoter of this gene. HBD-2 showed no direct antiviral activity against HRV. In vivo infection of normal human subjects with HRV-16 induced expression of mRNA for HBD-2 in nasal epithelial scrapings. Increases in mRNA correlated with viral titer and with increased levels of HBD-2 protein in nasal lavages. This represents the first demonstration that HRV infection induces epithelial expression of HBD-2 both in vitro and in vivo, and supports the concept that HBD-2 may play a role in host defense to HRV infection.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Proud, R. B. Turner, B. Winther, S. Wiehler, J. P. Tiesman, T. D. Reichling, K. D. Juhlin, A. W. Fulmer, B. Y. Ho, A. A. Walanski, et al.
Gene Expression Profiles during In Vivo Human Rhinovirus Infection: Insights into the Host Response
Am. J. Respir. Crit. Care Med., November 1, 2008; 178(9): 962 - 968.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
G. Diamond, N. Beckloff, and L.K. Ryan
Host Defense Peptides in the Oral Cavity and the Lung: Similarities and Differences
Journal of Dental Research, October 1, 2008; 87(10): 915 - 927.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R. L. Anderson, P. S. Hiemstra, C. Ward, I. A. Forrest, D. Murphy, D. Proud, J. Lordan, P. A. Corris, and A. J. Fisher
Antimicrobial peptides in lung transplant recipients with bronchiolitis obliterans syndrome
Eur. Respir. J., September 1, 2008; 32(3): 670 - 677.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. White
Vitamin D Signaling, Infectious Diseases, and Regulation of Innate Immunity
Infect. Immun., September 1, 2008; 76(9): 3837 - 3843.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
L. A. Smyth, N. Harker, W. Turnbull, H. El-Doueik, L. Klavinskis, D. Kioussis, G. Lombardi, and R. Lechler
The Relative Efficiency of Acquisition of MHC:Peptide Complexes and Cross-Presentation Depends on Dendritic Cell Type
J. Immunol., September 1, 2008; 181(5): 3212 - 3220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kota, A. Sabbah, T. H. Chang, R. Harnack, Y. Xiang, X. Meng, and S. Bose
Role of Human {beta}-Defensin-2 during Tumor Necrosis Factor-{alpha}/NF-{kappa}B-mediated Innate Antiviral Response against Human Respiratory Syncytial Virus
J. Biol. Chem., August 15, 2008; 283(33): 22417 - 22429.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Lau, X. Wang, L. Song, M. North, S. Wiehler, D. Proud, and C.-W. Chow
Syk Associates with Clathrin and Mediates Phosphatidylinositol 3-Kinase Activation during Human Rhinovirus Internalization
J. Immunol., January 15, 2008; 180(2): 870 - 880.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
J. Harder, R. Glaser, and J.-M. Schroder
Review: Human antimicrobial proteins effectors of innate immunity
Innate Immunity, December 1, 2007; 13(6): 317 - 338.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Wiehler and D. Proud
Interleukin-17A modulates human airway epithelial responses to human rhinovirus infection
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L505 - L515.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. Newton, N. S. Holden, M. C. Catley, W. Oyelusi, R. Leigh, D. Proud, and P. J. Barnes
Repression of Inflammatory Gene Expression in Human Pulmonary Epithelial Cells by Small-Molecule I{kappa}B Kinase Inhibitors
J. Pharmacol. Exp. Ther., May 1, 2007; 321(2): 734 - 742.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Wang, C. Lau, S. Wiehler, A. Pow, T. Mazzulli, C. Gutierrez, D. Proud, and C.-W. Chow
Syk Is Downstream of Intercellular Adhesion Molecule-1 and Mediates Human Rhinovirus Activation of p38 MAPK in Airway Epithelial Cells
J. Immunol., November 15, 2006; 177(10): 6859 - 6870.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. Proud and C.-W. Chow
Role of Viral Infections in Asthma and Chronic Obstructive Pulmonary Disease
Am. J. Respir. Cell Mol. Biol., November 1, 2006; 35(5): 513 - 518.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
P. Mallia and S. L. Johnston
How viral infections cause exacerbation of airway diseases.
Chest, October 1, 2006; 130(4): 1203 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Voss, J. Wehkamp, K. Wehkamp, E. F. Stange, J. M. Schroder, and J. Harder
NOD2/CARD15 Mediates Induction of the Antimicrobial Peptide Human Beta-defensin-2
J. Biol. Chem., January 27, 2006; 281(4): 2005 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Feng, N. Huang, Q. Wu, and B. Wang
HMGN2: a novel antimicrobial effector molecule of human mononuclear leukocytes?
J. Leukoc. Biol., November 1, 2005; 78(5): 1136 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. D. Starner, B. Agerberth, G. H. Gudmundsson, and P. B. McCray Jr.
Expression and Activity of {beta}-Defensins and LL-37 in the Developing Human Lung
J. Immunol., February 1, 2005; 174(3): 1608 - 1615.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.