| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Pathology and
Cell Biology and Physiology, University of New Mexico, Albuquerque, NM 87131
In the present study, we investigated the effects of in vivo Flt3L administration on the generation, phenotype, and function of lung dendritic cells (DCs) to evaluate whether Flt3L favors the expansion and maturation of a particular DC subset. Injection of Flt3L into mice resulted in an increased number of CD11c-expressing lung DCs, preferentially in the alveolar septa. FACS analysis allowed us to quantify a 19-fold increase in the absolute numbers of CD11c-positive, CD45R/B220 negative DCs in the lungs of Flt3L-treated mice over vehicle-treated mice. Further analysis revealed a 90-fold increase in the absolute number of myeloid DCs (CD11c positive, CD45R/B220 negative, and CD11b positive) and only a 3-fold increase of lymphoid DCs (CD11c positive, CD45R/B220 negative, and CD11b negative) from the lungs of Flt3L-treated mice over vehicle-treated mice. Flt3L-treated lung DCs were more mature than vehicle-treated lung DCs as demonstrated by a significantly higher percentage of cells expressing MHC class II, CD86, and CD40. Freshly isolated Flt3L lung DCs were not fully mature, because after an overnight culture they continued to increase accessory molecule expression. Functionally, Flt3L-treated lung DCs were more efficient than vehicle-treated DCs at stimulating naive T cell proliferation. Our data show that administration of Flt3L favors the expansion of myeloid lung DCs over lymphoid DCs and enhanced their ability to stimulate naive lymphocytes.
This article has been cited by other articles:
|
|
 |
|
  S. Sekine, K. Kataoka, Y. Fukuyama, Y. Adachi, J. Davydova, M. Yamamoto, R. Kobayashi, K. Fujihashi, H. Suzuki, D. T. Curiel, et al. A Novel Adenovirus Expressing Flt3 Ligand Enhances Mucosal Immunity by Inducing Mature Nasopharyngeal-Associated Lymphoreticular Tissue Dendritic Cell Migration J. Immunol., June 15, 2008; 180(12): 8126 - 8134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Hao, T. S. Kim, and T. J. Braciale Differential Response of Respiratory Dendritic Cell Subsets to Influenza Virus Infection J. Virol., May 15, 2008; 82(10): 4908 - 4919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. von Wulffen, M. Steinmueller, S. Herold, L. M. Marsh, P. Bulau, W. Seeger, T. Welte, J. Lohmeyer, and U. A. Maus Lung Dendritic Cells Elicited by Fms-like Tyrosin 3-Kinase Ligand Amplify the Lung Inflammatory Response to Lipopolysaccharide Am. J. Respir. Crit. Care Med., November 1, 2007; 176(9): 892 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takenaka, E. Safroneeva, Z. Xing, and J. Gauldie Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics J. Immunol., June 15, 2007; 178(12): 7984 - 7993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Beaty, C. E. Rose Jr., and S.-s. J. Sung Diverse and Potent Chemokine Production by Lung CD11bhigh Dendritic Cells in Homeostasis and in Allergic Lung Inflammation J. Immunol., February 1, 2007; 178(3): 1882 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, N. Peters, V. Laza-Stanca, N. Nawroly, S. L. Johnston, and J. Schwarze Local CD11c+ MHC Class II- Precursors Generate Lung Dendritic Cells during Respiratory Viral Infection, but Are Depleted in the Process J. Immunol., August 15, 2006; 177(4): 2536 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Bleier, S. C. Katz, U. I. Chaudhry, V. G. Pillarisetty, T. P. Kingham III, A. B. Shah, J. R. Raab, and R. P. DeMatteo Biliary obstruction selectively expands and activates liver myeloid dendritic cells. J. Immunol., June 15, 2006; 176(12): 7189 - 7195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-S. J. Sung, S. M. Fu, C. E. Rose Jr., F. Gaskin, S.-T. Ju, and S. R. Beaty A Major Lung CD103 ({alpha}E)-beta7 Integrin-Positive Epithelial Dendritic Cell Population Expressing Langerin and Tight Junction Proteins J. Immunol., February 15, 2006; 176(4): 2161 - 2172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Vermaelen and R. Pauwels Pulmonary Dendritic Cells Am. J. Respir. Crit. Care Med., September 1, 2005; 172(5): 530 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ali, G. D. King, J. F. Curtin, M. Candolfi, W. Xiong, C. Liu, M. Puntel, Q. Cheng, J. Prieto, A. Ribas, et al. Combined Immunostimulation and Conditional Cytotoxic Gene Therapy Provide Long-term Survival in a Large Glioma Model Cancer Res., August 15, 2005; 65(16): 7194 - 7204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Padilla, E. Daley, A. Chow, K. Robinson, K. Parthasarathi, A. N. J. McKenzie, T. Tschernig, V. P. Kurup, D. D. Donaldson, and G. Grunig IL-13 Regulates the Immune Response to Inhaled Antigens J. Immunol., June 15, 2005; 174(12): 8097 - 8105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Toliver-Kinsky, W. Cui, E. D. Murphey, C. Lin, and E. R. Sherwood Enhancement of Dendritic Cell Production by Fms-Like Tyrosine Kinase-3 Ligand Increases the Resistance of Mice to a Burn Wound Infection J. Immunol., January 1, 2005; 174(1): 404 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Swanson, Y. Zheng, K. M. Heidler, Z.-D. Zhang, T. J. Webb, and D. S. Wilkes Flt3-Ligand, IL-4, GM-CSF, and Adherence-Mediated Isolation of Murine Lung Dendritic Cells: Assessment of Isolation Technique on Phenotype and Function J. Immunol., October 15, 2004; 173(8): 4875 - 4881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Tobar, P. A. Gonzalez, and A. M. Kalergis Salmonella Escape from Antigen Presentation Can Be Overcome by Targeting Bacteria to Fc{gamma} Receptors on Dendritic Cells J. Immunol., September 15, 2004; 173(6): 4058 - 4065. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
