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A Major Allergen from Pollen Defines a Novel Family of Plant Proteins and Shows Intra- and Interspecie Cross-Reactivity¹

Patricia Barral^*, Eva Batanero^*, Oscar Palomares^*, Joaquín Quiralte, Mayte Villalba^* and Rosalía Rodríguez^*,2

^* Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Madrid, Spain; and Unidad de Alergia, Complejo Hospitalario de Jaén, Jaén, Spain

Olive tree (Olea europaea) pollen is a main cause of allergy associated with extensive areas of Europe and North America. Ole e 10, a small (10.8 kDa) and acidic (pI 5.8) protein, has been identified as a major allergen from the olive pollen, isolated, and characterized. Circular dichroism analysis gave 17% helix, 33% sheet, and 21% turn for its secondary structure. Based on amino acid sequences of tryptic peptides, the protein was cloned and sequenced. The allergen consists of a single polypeptide chain of 102 aa, with a signal peptide of 21 residues. Ole e 10 showed homology with the C-terminal domain of another olive allergen, Ole e 9 (1,3--glucanase, 53% identity), with deduced sequences from Arabidopsis thaliana genes (42–46% identity) and with polypeptide segments (Cys boxes) of proteins involved in yeast development (Epd1/Gas-1p/Phr2 families; 42–43% similarity). Ole e 10 showed 55% prevalence for olive-allergic patients and exhibited an IgE response dependent on its conformation. Remarkable IgE cross-reactivity was detected with Ole e 9, but no correlation was observed between the individual IgE responses to both allergens. Ole e 10 shares IgE B cell epitopes with proteins from Oleaceae, Gramineae, Betulaceae, Chenopodiaceae, Cupressaceae, Ambrosia, and Parietaria pollens, latex, and vegetable foods, such as tomato, kiwi, potato, and peach. These data indicate that Ole e 10 is a new pan-allergenic plant protein that shows notable intra- and interspecie IgE cross-reactivity and is a powerful candidate to be involved in pollen-latex-fruit syndrome.

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				M. A. Trevino, O. Palomares, I. Castrillo, M. Villalba, R. Rodriguez, M. Rico, J. Santoro, and M. Bruix Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen Protein Sci., February 1, 2008; 17(2): 371 - 376. [Abstract] [Full Text] [PDF]

				M. A. Trevino, M. F. Garcia-Mayoral, P. Barral, M. Villalba, J. Santoro, M. Rico, R. Rodriguez, and M. Bruix NMR Solution Structure of Ole e 6, a Major Allergen from Olive Tree Pollen J. Biol. Chem., September 10, 2004; 279(37): 39035 - 39041. [Abstract] [Full Text] [PDF]