HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roberts, T. J. Articles by Brutkiewicz, R. R. Search for Related Content PubMed PubMed Citation Articles by Roberts, T. J. Articles by Brutkiewicz, R. R.

CD1d1-Dependent Control of the Magnitude of an Acute Antiviral Immune Response¹

Tonya J. Roberts^2,*, Yinling Lin^2,*, Philip M. Spence^*, Luc Van Kaer and Randy R. Brutkiewicz^3,*

^* Department of Microbiology and Immunology, Indiana University School of Medicine, and The Walther Oncology Center, Indianapolis, IN 46202; The Walther Cancer Institute, Indianapolis, IN 46208; and Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232

CD1d1-restricted NK T (NKT) cells rapidly secrete both Th1 and Th2 cytokines upon activation and are therefore thought to play a regulatory role during an immune response. In this study we examined the role of CD1d1 molecules and NKT cells in regulating virus-induced cytokine production. CD1d1-deficient (CD1KO) mice, which lack NKT cells, were infected with lymphocytic choriomeningitis virus, and spontaneous cytokine release from splenocytes was measured. We found that CD1KO mice produce significantly higher amounts of IL-2, IL-4, and IFN- compared with wild-type controls postinfection. Depletion studies of individual lymphocyte subpopulations suggested that CD4⁺ T cells are required; however, isolation of specific lymphocyte populations indicated that CD4⁺ T cells alone are not sufficient for the increase in cytokine production in CD1KO mice. Splenocytes from lymphocytic choriomeningitis virus-infected CD1KO mice continued to produce enhanced cytokine levels long after viral clearance and cleared viral RNA faster than wild-type mice. There was no difference in the number of splenocytes between uninfected wild-type and CD1KO mice, whereas the latter knockout mice had an increased number of splenocytes after infection. Collectively, these data provide clear evidence that the expression of CD1d1 molecules controls the magnitude of the cell-mediated immune response to an acute viral infection.

This article has been cited by other articles:

				I. Tsunoda, T. Tanaka, and R. S. Fujinami Regulatory Role of CD1d in Neurotropic Virus Infection J. Virol., October 15, 2008; 82(20): 10279 - 10289. [Abstract] [Full Text] [PDF]

				P. Bochtler, A. Kroger, R. Schirmbeck, and J. Reimann Type I IFN-Induced, NKT Cell-Mediated Negative Control of CD8 T Cell Priming by Dendritic Cells J. Immunol., August 1, 2008; 181(3): 1633 - 1643. [Abstract] [Full Text] [PDF]

				E Zigmond, S Preston, O Pappo, G Lalazar, M Margalit, Z Shalev, L Zolotarov, D Friedman, R Alper, and Y Ilan {beta}-Glucosylceramide: a novel method for enhancement of natural killer T lymphoycte plasticity in murine models of immune-mediated disorders Gut, January 1, 2007; 56(1): 82 - 89. [Abstract] [Full Text] [PDF]

				P. O. Ilyinskii, R. Wang, S. P. Balk, and M. A. Exley CD1d Mediates T-Cell-Dependent Resistance to Secondary Infection with Encephalomyocarditis Virus (EMCV) In Vitro and Immune Response to EMCV Infection In Vivo J. Virol., July 15, 2006; 80(14): 7146 - 7158. [Abstract] [Full Text] [PDF]

				R. R. Brutkiewicz CD1d Ligands: The Good, the Bad, and the Ugly J. Immunol., July 15, 2006; 177(2): 769 - 775. [Abstract] [Full Text] [PDF]

				S. T. Smiley, P. A. Lanthier, K. N. Couper, F. M. Szaba, J. E. Boyson, W. Chen, and L. L. Johnson Exacerbated Susceptibility to Infection-Stimulated Immunopathology in CD1d-Deficient Mice J. Immunol., June 15, 2005; 174(12): 7904 - 7911. [Abstract] [Full Text] [PDF]

				B. Chung, A. Aoukaty, J. Dutz, C. Terhorst, and R. Tan Cutting Edge: Signaling Lymphocytic Activation Molecule-Associated Protein Controls NKT Cell Functions J. Immunol., March 15, 2005; 174(6): 3153 - 3157. [Abstract] [Full Text] [PDF]

				Y. Lin, T. J. Roberts, P. M. Spence, and R. R. Brutkiewicz Reduction in CD1d expression on dendritic cells and macrophages by an acute virus infection J. Leukoc. Biol., February 1, 2005; 77(2): 151 - 158. [Abstract] [Full Text] [PDF]

				M. S. Duthie, M. Kahn, M. White, R. P. Kapur, and S. J. Kahn Critical Proinflammatory and Anti-Inflammatory Functions of Different Subsets of CD1d-Restricted Natural Killer T Cells during Trypanosoma cruzi Infection Infect. Immun., January 1, 2005; 73(1): 181 - 192. [Abstract] [Full Text] [PDF]