| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Rheumatology Section, Division of Medicine, Faculty of Medicine, Imperial College, Hammersmith Campus, London, United Kingdom; and
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
Human CD93 (known as C1qRp) has been shown to be a phagocytic receptor involved in the in vitro C1q-dependent enhancement of phagocytosis. However, binding of CD93 to C1q and its function remain controversial. In this study, we have generated CD93-deficient mice (CD93-/-) to investigate its biological role(s). The CD93-/- mice were viable and showed no gross abnormalities in their development. Thioglycolate-elicited peritoneal macrophages deficient in CD93 showed a similar enhancement in complement- and Fc
R-dependent uptake of RBC to the wild-type macrophages when plated on C1q-coated surfaces suggesting that the lack of this receptor had no effect on these C1q-mediated events. There was no impairment in either complement- or FcR-dependent phagocytic assays in vivo. By contrast, the CD93-/- mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo (human Jurkat T cells and murine thymocytes: p = 0.0006 and p = 0.0079, respectively) compared with strain-matched controls. However, in vitro, the CD93-/- macrophages showed similar engulfment of apoptotic cells to wild-type macrophages. Furthermore, no supporting evidence for a role of CD93 as an adhesion molecule was found using intravital microscopy or analyzing peritoneal cell recruitment in response to three different inflammatory stimuli (thioglycolate, zymosan A, and IL-1
). Thus, our findings indicate that murine CD93 is expressed on the peritoneal macrophage, especially on thioglycolate-elicited cells, but does not appear to play a key role in C1q-mediated enhancement of phagocytosis or in the intercellular adhesion events tested. However, our results suggest that it may contribute to the in vivo clearance of dying cells.
Related articles in The JI:
This article has been cited by other articles:
|
|
 |
|
  S. Chevrier, C. Genton, A. Kallies, A. Karnowski, L. A. Otten, B. Malissen, M. Malissen, M. Botto, L. M. Corcoran, S. L. Nutt, et al. CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche PNAS, March 10, 2009; 106(10): 3895 - 3900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Lillis, M. C. Greenlee, I. Mikhailenko, S. V. Pizzo, A. J. Tenner, D. K. Strickland, and S. S. Bohlson Murine Low-Density Lipoprotein Receptor-Related Protein 1 (LRP) Is Required for Phagocytosis of Targets Bearing LRP Ligands but Is Not Required for C1q-Triggered Enhancement of Phagocytosis J. Immunol., July 1, 2008; 181(1): 364 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. McCall-Culbreath, Z. Li, and M. M. Zutter Crosstalk between the {alpha}2{beta}1 integrin and c-met/HGF-R regulates innate immunity Blood, April 1, 2008; 111(7): 3562 - 3570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wan, M. A. Versnel, W. y. Cheung, P. J. M. Leenen, N. A. Khan, R. Benner, and R. C. M. Kiekens Chorionic gonadotropin can enhance innate immunity by stimulating macrophage function J. Leukoc. Biol., October 1, 2007; 82(4): 926 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Seitz, T. D. Camenisch, G. Lemke, H. S. Earp, and G. K. Matsushima Macrophages and Dendritic Cells Use Different Axl/Mertk/Tyro3 Receptors in Clearance of Apoptotic Cells J. Immunol., May 1, 2007; 178(9): 5635 - 5642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Edelson, T. P. Stricker, Z. Li, S. K. Dickeson, V. L. Shepherd, S. A. Santoro, and M. M. Zutter Novel collectin/C1q receptor mediates mast cell activation and innate immunity Blood, January 1, 2006; 107(1): 143 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Bohlson, R. Silva, M. I. Fonseca, and A. J. Tenner CD93 Is Rapidly Shed from the Surface of Human Myeloid Cells and the Soluble Form Is Detected in Human Plasma J. Immunol., July 15, 2005; 175(2): 1239 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Bohlson, M. Zhang, C. E. Ortiz, and A. J. Tenner CD93 interacts with the PDZ domain-containing adaptor protein GIPC: implications in the modulation of phagocytosis J. Leukoc. Biol., January 1, 2005; 77(1): 80 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Stokol, P. O'Donnell, L. Xiao, S. Knight, G. Stavrakis, M. Botto, U. H. von Andrian, and T. N. Mayadas C1q Governs Deposition of Circulating Immune Complexes and Leukocyte Fc{gamma} Receptors Mediate Subsequent Neutrophil Recruitment J. Exp. Med., October 4, 2004; 200(7): 835 - 846. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
