| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute, London, United Kingdom; and
Central Laboratory of Netherlands, Netherlands Red Cross, Blood Transfusion Service, Amsterdam, The Netherlands
T regulatory cells and IL-10 have been implicated in the mechanism of immunotherapy in patients with systemic anaphylaxis following bee stings. We studied the role of IL-10 in the induction of clinical, cellular, and humoral tolerance during immunotherapy for local mucosal allergy in subjects with seasonal pollinosis. Local and systemic IL-10 responses and serum Ab concentrations were measured before/after a double-blind trial of grass pollen (Phleum pratense, Phl P) immunotherapy. We observed local increases in IL-10 mRNA-positive cells in the nasal mucosa after 2 years of immunotherapy, but only during the pollen season. IL-10 protein-positive cells were also increased and correlated with IL-10 mRNA+ cells. These changes were not observed in placebo-treated subjects or in healthy controls. Fifteen and 35% of IL-10 mRNA signals were colocalized to CD3+ T cells and CD68+ macrophages, respectively, whereas only 1–2% of total CD3+ cells and 4% of macrophages expressed IL-10. Following immunotherapy, peripheral T cells cultured in the presence of grass pollen extract also produced IL-10. Immunotherapy resulted in blunting of seasonal increases in serum allergen Phl p 5-specific IgE, 60- to 80-fold increases in Phl p 5-specific IgG, and 100-fold increases in Phl p 5-specific IgG4. Post-immunotherapy serum exhibited inhibitory activity, which coeluted with IgG4, and blocked IgE-facilitated binding of allergen-IgE complexes to B cells. Both the increases in IgG and the IgG "blocking" activity correlated with the patients’ overall assessment of improvement. Thus, grass pollen immunotherapy may induce allergen-specific, IL-10-dependent "protective" IgG4 responses.
This article has been cited by other articles:
|
|
 |
|
  J. D. Campbell, K. F. Buckland, S. J. McMillan, J. Kearley, W. L.G. Oldfield, L. J. Stern, H. Gronlund, M. van Hage, C. J. Reynolds, R. J. Boyton, et al. Peptide immunotherapy in allergic asthma generates IL-10-dependent immunological tolerance associated with linked epitope suppression J. Exp. Med., July 6, 2009; 206(7): 1535 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Dragos, M Jones, J-L Malo, H Ghezzo, and D Gautrin Specific antibodies to diisocyanate and work-related respiratory symptoms in apprentice car-painters Occup. Environ. Med., April 1, 2009; 66(4): 227 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gerstmayr, N. Ilk, I. Schabussova, B. Jahn-Schmid, E. M. Egelseer, U. B. Sleytr, C. Ebner, and B. Bohle A Novel Approach to Specific Allergy Treatment: The Recombinant Allergen-S-Layer Fusion Protein rSbsC-Bet v 1 Matures Dendritic Cells That Prime Th0/Th1 and IL-10-Producing Regulatory T Cells J. Immunol., December 1, 2007; 179(11): 7270 - 7275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pilette, K. T. Nouri-Aria, M. R. Jacobson, L. K. Wilcock, B. Detry, S. M. Walker, J. N. Francis, and S. R. Durham Grass Pollen Immunotherapy Induces an Allergen-Specific IgA2 Antibody Response Associated with Mucosal TGF-beta Expression J. Immunol., April 1, 2007; 178(7): 4658 - 4666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Piconi, D. Trabattoni, M. Saresella, E. Iemoli, M. Schenal, A. Fusi, M. Borelli, L. Chen, A. Mascheri, and M. Clerici Effects of Specific Immunotherapy on the B7 Family of Costimulatory Molecules in Allergic Inflammation J. Immunol., February 1, 2007; 178(3): 1931 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Creticos, J. T. Schroeder, R. G. Hamilton, S. L. Balcer-Whaley, A. P. Khattignavong, R. Lindblad, H. Li, R. Coffman, V. Seyfert, J. J. Eiden, et al. Immunotherapy with a Ragweed-Toll-Like Receptor 9 Agonist Vaccine for Allergic Rhinitis N. Engl. J. Med., October 5, 2006; 355(14): 1445 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. M. van Oosterhout and N. Bloksma Regulatory T-lymphocytes in asthma Eur. Respir. J., November 1, 2005; 26(5): 918 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. M. van Oosterhout and A. C. Motta Th1/Th2 paradigm: not seeing the forest for the trees? Eur. Respir. J., April 1, 2005; 25(4): 591 - 593. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
