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Departments of
*
Pediatrics and
Microbiology, University of Iowa, Iowa City, IA 52242; and
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada
We measured CD8 T cell clonotypic diversity to three epitopes recognized in C57BL/6 mice infected with mouse hepatitis virus, strain JHM, or lymphocytic choriomeningitis virus. We isolated epitope-specific T cells with an IFN-
capture assay or MHC class I/peptide tetramers and identified different clonotypes by V
chain sequence analysis. In agreement with our previous results, the number of different clonotypes responding to all three epitopes fit a log-series distribution. From these distributions, we estimated that >1000 different clonotypes responded to each immunodominant CD8 T cell epitope; the response to a subdominant CD8 T cell epitope was modestly less diverse. These results suggest that T cell response diversity is greater by 1–2 orders of magnitude than predicted previously.
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