The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dorner, B. G.
Right arrow Articles by Yokoyama, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorner, B. G.
Right arrow Articles by Yokoyama, W. M.
The Journal of Immunology, 2004, 172: 3119-3131.
Copyright © 2004 by The American Association of Immunologists

Coordinate Expression of Cytokines and Chemokines by NK Cells during Murine Cytomegalovirus Infection1

Brigitte G. Dorner2,*, Hamish R.C. Smith*, Anthony R. French*, Sungjin Kim*, Jennifer Poursine-Laurent*, Diana L. Beckman*, Jeanette T. Pingel*, Richard A. Kroczek{dagger} and Wayne M. Yokoyama3,*

* Howard Hughes Medical Institute and Division of Rheumatology, Department of Medicine, Washington University School of Medicine and Barnes-Jewish Hospital, St. Louis, MO 63110; and {dagger} Molecular Immunology, Robert Koch-Institute, Berlin, Germany

Cytokines and chemokines activate and direct effector cells during infection. We previously identified a functional group of five cytokines and chemokines, namely, IFN-{gamma}, activation-induced T cell-derived and chemokine-related cytokine/lymphotactin, macrophage-inflammatory protein 1{alpha}, macrophage-inflammatory protein 1{beta}, and RANTES, coexpressed in individual activated NK cells, CD8+ T cells, and CD4+ Th1 cells in vitro and during in vivo infections. However, the stimuli during infection were not known. In murine CMV (MCMV) infection, the DAP12/KARAP-associated Ly49H NK cell activation receptor is crucial for resistance through recognition of MCMV-encoded m157 but NK cells also undergo in vivo nonspecific responses to uncharacterized stimuli. In this study, we show that Ly49H ligation by m157 resulted in a coordinated release of all five cytokines/chemokines from Ly49H+ NK cells. Whereas other cytokines also triggered the release of these cytokines/chemokines, stimulation was not confined to the Ly49H+ population. At the single-cell level, the production of the five mediators showed strong positive correlation with each other. Interestingly, NK cells were a major source of these five cytokines/chemokines in vitro and in vivo, whereas infected macrophages produced only limited amounts of macrophage-inflammatory protein 1{alpha}, macrophage-inflammatory protein1{beta}, and RANTES. These findings suggest that both virus-specific and nonspecific NK cells play crucial roles in activating and directing other inflammatory cells during MCMV infection.




This article has been cited by other articles:


Home page
 J. Exp. Med.Home page
H. Guo, A. Samarakoon, B. Vanhaesebroeck, and S. Malarkannan
The p110{delta} of PI3K plays a critical role in NK cell terminal maturation and cytokine/chemokine generation
J. Exp. Med., September 29, 2008; 205(10): 2419 - 2435.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Malarkannan, J. Regunathan, H. Chu, S. Kutlesa, Y. Chen, H. Zeng, R. Wen, and D. Wang
Bcl10 Plays a Divergent Role in NK Cell-Mediated Cytotoxicity and Cytokine Generation
J. Immunol., September 15, 2007; 179(6): 3752 - 3762.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Divangahi, T. Yang, K. Kugathasan, S. McCormick, S. Takenaka, G. Gaschler, A. Ashkar, M. Stampfli, J. Gauldie, J. Bramson, et al.
Critical Negative Regulation of Type 1 T Cell Immunity and Immunopathology by Signaling Adaptor DAP12 during Intracellular Infection
J. Immunol., September 15, 2007; 179(6): 4015 - 4026.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. K. Parker, S. Parker, W. M. Yokoyama, J. A. Corbett, and R. M. L. Buller
Induction of Natural Killer Cell Responses by Ectromelia Virus Controls Infection
J. Virol., April 15, 2007; 81(8): 4070 - 4079.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Becknell, T. L. Hughes, A. G. Freud, B. W. Blaser, J. Yu, R. Trotta, H. C. Mao, M. L. Caligiuri de Jesus, M. Alghothani, D. M. Benson Jr, et al.
Hlx homeobox transcription factor negatively regulates interferon-{gamma} production in monokine-activated natural killer cells
Blood, March 15, 2007; 109(6): 2481 - 2487.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
J. Sawaki, H. Tsutsui, N. Hayashi, K. Yasuda, S. Akira, T. Tanizawa, and K. Nakanishi
Type 1 cytokine/chemokine production by mouse NK cells following activation of their TLR/MyD88-mediated pathways
Int. Immunol., March 1, 2007; 19(3): 311 - 320.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. R. French, H. Sjolin, S. Kim, R. Koka, L. Yang, D. A. Young, C. Cerboni, E. Tomasello, A. Ma, E. Vivier, et al.
DAP12 Signaling Directly Augments Proproliferative Cytokine Stimulation of NK Cells during Viral Infections
J. Immunol., October 15, 2006; 177(8): 4981 - 4990.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Regunathan, Y. Chen, S. Kutlesa, X. Dai, L. Bai, R. Wen, D. Wang, and S. Malarkannan
Differential and Nonredundant Roles of Phospholipase C{gamma}2 and Phospholipase C{gamma}1 in the Terminal Maturation of NK Cells
J. Immunol., October 15, 2006; 177(8): 5365 - 5376.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Marcenaro, F. Gallo, S. Martini, A. Santoro, G. M. Griffiths, M. Arico, L. Moretta, and D. Pende
Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease
Blood, October 1, 2006; 108(7): 2316 - 2323.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Pende, R. Castriconi, P. Romagnani, G. M. Spaggiari, S. Marcenaro, A. Dondero, E. Lazzeri, L. Lasagni, S. Martini, P. Rivera, et al.
Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction
Blood, March 1, 2006; 107(5): 2030 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. M. Roda, R. Parihar, C. Magro, G. J. Nuovo, S. Tridandapani, and W. E. Carson III
Natural Killer Cells Produce T Cell-Recruiting Chemokines in Response to Antibody-Coated Tumor Cells
Cancer Res., January 1, 2006; 66(1): 517 - 526.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Regunathan, Y. Chen, D. Wang, and S. Malarkannan
NKG2D receptor-mediated NK cell function is regulated by inhibitory Ly49 receptors
Blood, January 1, 2005; 105(1): 233 - 240.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Hanna, P. Bechtel, Y. Zhai, F. Youssef, K. McLachlan, and O. Mandelboim
Novel Insights on Human NK Cells' Immunological Modalities Revealed by Gene Expression Profiling
J. Immunol., December 1, 2004; 173(11): 6547 - 6563.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Ramakrishna, S. A. Stohlman, R. A. Atkinson, D. R. Hinton, and C. C. Bergmann
Differential Regulation of Primary and Secondary CD8+ T Cells in the Central Nervous System
J. Immunol., November 15, 2004; 173(10): 6265 - 6273.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.