The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johansson, C.
Right arrow Articles by Wick, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johansson, C.
Right arrow Articles by Wick, M. J.
The Journal of Immunology, 2004, 172: 2496-2503.
Copyright © 2004 by The American Association of Immunologists

Liver Dendritic Cells Present Bacterial Antigens and Produce Cytokines upon Salmonella Encounter 1

Cecilia Johansson* and Mary Jo Wick2,*,{dagger}

* Department of Cell and Molecular Biology, Section for Immunology, Lund University, Lund, Sweden, and {dagger} Department of Clinical Immunology, Göteborg University, Göteborg, Sweden

The capacity of murine liver dendritic cells (DC) to present bacterial Ags and produce cytokines after encounter with Salmonella was studied. Freshly isolated, nonparenchymal liver CD11c+ cells had heterogeneous expression of MHC class II and CD11b and a low level of CD40 and CD86 expression. Characterization of liver DC subsets revealed that CD8{alpha}-CD4- double negative cells constituted the majority of liver CD11c+ (~85%) with few cells expressing CD8{alpha} or CD4. Flow cytometry analysis of freshly isolated CD11c+ cells enriched from the liver and cocultured with Salmonella expressing green fluorescent protein (GFP) showed that CD11c+ MHC class IIhigh cells had a greater capacity to internalize Salmonella relative to CD11c+ MHC class IIlow cells. Moreover, both CD8{alpha}- and CD8{alpha}+ liver DC internalized bacteria with similar efficiency after both in vitro and in vivo infection. CD11c+ cells enriched from the liver could also process Salmonella for peptide presentation on MHC class I and class II to primary, Ag-specific T cells after internalization requiring actin cytoskeletal rearrangements. Flow cytometry analysis of liver CD11c+ cells infected with Salmonella expressing GFP showed that both CD8{alpha}- and CD8{alpha}+ DC produced IL-12p40 and TNF-{alpha}. The majority of cytokine-positive cells did not contain bacteria (GFP-) whereas only a minor fraction of cytokine-positive cells were GFP+. Furthermore, only ~30–50% of liver DC containing bacteria (GFP+) produced cytokines. Thus, liver DC can internalize and process Salmonella for peptide presentation to CD4+ and CD8+ T cells and elicit proinflammatory cytokine production upon Salmonella encounter, suggesting that DC in the liver may contribute to immunity against hepatotropic bacteria.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
S. S. M. Chan, P. Mastroeni, I. McConnell, and B. A. Blacklaws
Salmonella infection of afferent lymph dendritic cells
J. Leukoc. Biol., February 1, 2008; 83(2): 272 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. A. Herrada, F. J. Contreras, J. A. Tobar, R. Pacheco, and A. M. Kalergis
Immune complex-induced enhancement of bacterial antigen presentation requires Fc{gamma} Receptor III expression on dendritic cells
PNAS, August 14, 2007; 104(33): 13402 - 13407.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Rydstrom and M. J. Wick
Monocyte Recruitment, Activation, and Function in the Gut-Associated Lymphoid Tissue during Oral Salmonella Infection
J. Immunol., May 1, 2007; 178(9): 5789 - 5801.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. I. Bleier, S. C. Katz, U. I. Chaudhry, V. G. Pillarisetty, T. P. Kingham III, A. B. Shah, J. R. Raab, and R. P. DeMatteo
Biliary obstruction selectively expands and activates liver myeloid dendritic cells.
J. Immunol., June 15, 2006; 176(12): 7189 - 7195.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. A. Knodler, A. Bestor, C. Ma, I. Hansen-Wester, M. Hensel, B. A. Vallance, and O. Steele-Mortimer
Cloning Vectors and Fluorescent Proteins Can Significantly Inhibit Salmonella enterica Virulence in Both Epithelial Cells and Macrophages: Implications for Bacterial Pathogenesis Studies
Infect. Immun., October 1, 2005; 73(10): 7027 - 7031.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Sundquist and M. J. Wick
TNF-{alpha}-Dependent and -Independent Maturation of Dendritic Cells and Recruited CD11cintCD11b+ Cells during Oral Salmonella Infection
J. Immunol., September 1, 2005; 175(5): 3287 - 3298.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. C. Kirby, M. Sundquist, and M. J. Wick
In Vivo Compartmentalization of Functionally Distinct, Rapidly Responsive Antigen-Specific T-Cell Populations in DNA-Immunized or Salmonella enterica Serovar Typhimurium-Infected Mice
Infect. Immun., November 1, 2004; 72(11): 6390 - 6400.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. A. Tobar, P. A. Gonzalez, and A. M. Kalergis
Salmonella Escape from Antigen Presentation Can Be Overcome by Targeting Bacteria to Fc{gamma} Receptors on Dendritic Cells
J. Immunol., September 15, 2004; 173(6): 4058 - 4065.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.