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Biases in Ig Light Chain Rearrangements in Human Intestinal Plasma Cells ¹

Wen Su^*, Laurent Boursier^*, Archana Padala^*, Jeremy D. Sanderson and Jo Spencer^2,*

^* Department of Histopathology, GKT Medical School, St. Thomas’ Campus, London, United Kingdom; and Department of Gastroenterology, St. Thomas’ Hospital, London, United Kingdom

Human intestinal lamina propria plasma cells are considered to be the progeny of chronically stimulated germinal centers located in organized gut-associated lymphoid tissues such as Peyer’s patches and isolated lymphoid follicles. We have sampled human colonic lamina propria plasma cells and naive and memory B cell subsets from human Peyer’s patches by microdissection of immunohistochemically stained tissue sections and used PCR methods and sequence analysis to compare IgVJ rearrangements in the plasma cell and B cell populations. Rearrangements that were either in-frame or out-of-frame between V and J were compared. Usage of IgV families in the in-frame rearrangements from the plasma cells resembled that observed in the mantle cells, suggesting that antigenic selection for cellular specificity does not dramatically favor any particular V segment. However, in marked contrast, out-of-frame rearrangements involving V1 and V2 families are rarely observed in intestinal plasma cells, whereas rearrangements involving V5 are increased. This resulted in significantly biased ratios of in-frame:out-of-frame rearrangements in these V families. Out-of-frame rearrangements of IgVJ from plasma cells, including those involving the V5 family, have a significant tendency not to involve J1, consistent with the hypothesis that this population includes rearrangements generated by secondary recombination events. We propose that modification of out-of-frame rearrangements of IgVJ exists, probably a consequence of secondary rearrangements. This may be a mechanism to avoid translocations to susceptible out-of-frame IgVJ rearrangements during somatic hypermutation.

This article has been cited by other articles:

				W. Su, J. N. Gordon, F. Barone, L. Boursier, W. Turnbull, S. Mendis, D. K. Dunn-Walters, and J. Spencer Lambda Light Chain Revision in the Human Intestinal IgA Response J. Immunol., July 15, 2008; 181(2): 1264 - 1271. [Abstract] [Full Text] [PDF]