The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibson, L.
Right arrow Articles by Luzuriaga, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibson, L.
Right arrow Articles by Luzuriaga, K.
The Journal of Immunology, 2004, 172: 2256-2264.
Copyright © 2004 by The American Association of Immunologists

Human Cytomegalovirus Proteins pp65 and Immediate Early Protein 1 Are Common Targets for CD8+ T Cell Responses in Children with Congenital or Postnatal Human Cytomegalovirus Infection1

Laura Gibson2,*,{dagger}, Giampiero Piccinini§, Daniele Lilleri§, Maria Grazia Revello§, Zhongde Wang, Susan Markel, Don J. Diamond and Katherine Luzuriaga*,{ddagger}

Departments of * Pediatrics and {dagger} Medicine and {ddagger} Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605; § Servizio di Virologia, Istituto de Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy; and Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, CA 91010

Recombinant modified vaccinia Ankara- and peptide-based IFN-{gamma} ELISPOT assays were used to detect and measure human CMV (HCMV)-specific CD8+ T cell responses to the pp65 (UL83) and immediate early protein 1 (IE1; UL123) gene products in 16 HCMV-infected infants and children. Age at study ranged from birth to 2 years. HCMV-specific CD8+ T cells were detected in 14 (88%) of 16 children at frequencies ranging from 60 to >2000 spots/million PBMC. Responses were detected as early as 1 day of age in infants with documented congenital infection. Nine children responded to both pp65 and IE1, whereas responses to pp65 or IE1 alone were detected in three and two children, respectively. Regardless of the specificity of initial responses, IE1-specific responses predominated by 1 year of age. Changes in HCMV epitopes targeted by the CD8+ T cell responses were observed over time; epitopes commonly recognized by HLA-A2+ adults with latent HCMV infection did not fully account for responses detected in early childhood. Finally, the detection of HCMV-specific CD8+ T cell responses was temporally associated with a decrease in peripheral blood HCMV load. Taken altogether, these data demonstrate that the fetus and young infant can generate virus-specific CD8+ T cell responses. Changes observed in the protein and epitope-specificity of HCMV-specific CD8+ T cells over time are consistent with those observed after other primary viral infections. The temporal association between the detection of HCMV-specific CD8+ T cell responses and the reduction in blood HCMV load supports the importance of CD8+ T cells in controlling primary HCMV viremia.




This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
M. C.-J. Cheeran, J. R. Lokensgard, and M. R. Schleiss
Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention
Clin. Microbiol. Rev., January 1, 2009; 22(1): 99 - 126.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Wilhelmi, C. O. Simon, J. Podlech, V. Bohm, T. Daubner, S. Emde, D. Strand, A. Renzaho, N. A. W. Lemmermann, C. K. Seckert, et al.
Transactivation of Cellular Genes Involved in Nucleotide Metabolism by the Regulatory IE1 Protein of Murine Cytomegalovirus Is Not Critical for Viral Replicative Fitness in Quiescent Cells and Host Tissues
J. Virol., October 15, 2008; 82(20): 9900 - 9916.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. R. B. Bantug, D. Cekinovic, R. Bradford, T. Koontz, S. Jonjic, and W. J. Britt
CD8+ T Lymphocytes Control Murine Cytomegalovirus Replication in the Central Nervous System of Newborn Animals
J. Immunol., August 1, 2008; 181(3): 2111 - 2123.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. R. Pipeling, E. E. West, C. M. Osborne, A. B. Whitlock, L. K. Dropulic, M. H. Willett, M. Forman, A. Valsamakis, J. B. Orens, D. R. Moller, et al.
Differential CMV-Specific CD8+ Effector T Cell Responses in the Lung Allograft Predominate over the Blood during Human Primary Infection
J. Immunol., July 1, 2008; 181(1): 546 - 556.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. S. Cobbs, L. Soroceanu, S. Denham, W. Zhang, and M. H. Kraus
Modulation of Oncogenic Phenotype in Human Glioma Cells by Cytomegalovirus IE1-Mediated Mitogenicity
Cancer Res., February 1, 2008; 68(3): 724 - 730.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
J. R. Currier, L. M. Galley, H. Wenschuh, V. Morafo, S. Ratto-Kim, C. M. Gray, L. Maboko, M. Hoelscher, M. A. Marovich, and J. H. Cox
Peptide Impurities in Commercial Synthetic Peptides and Their Implications for Vaccine Trial Assessment
Clin. Vaccine Immunol., February 1, 2008; 15(2): 267 - 276.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. W. Munks, K. S. Cho, A. K. Pinto, S. Sierro, P. Klenerman, and A. B. Hill
Four Distinct Patterns of Memory CD8 T Cell Responses to Chronic Murine Cytomegalovirus Infection
J. Immunol., July 1, 2006; 177(1): 450 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Sanchez-Merino, S. Nie, and K. Luzuriaga
HIV-1-Specific CD8+ T Cell Responses and Viral Evolution in Women and Infants
J. Immunol., November 15, 2005; 175(10): 6976 - 6986.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. W. Sylwester, B. L. Mitchell, J. B. Edgar, C. Taormina, C. Pelte, F. Ruchti, P. R. Sleath, K. H. Grabstein, N. A. Hosken, F. Kern, et al.
Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects
J. Exp. Med., September 6, 2005; 202(5): 673 - 685.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. D. Reuter, J. H. Wilson, K. E Idoko, and A. N. van den Pol
CD4+ T-Cell Reconstitution Reduces Cytomegalovirus in the Immunocompromised Brain
J. Virol., August 1, 2005; 79(15): 9527 - 9539.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. N. Posnett, M. E. Engelhorn, and A. N. Houghton
Antiviral T cell responses: phalanx or multipronged attack?
J. Exp. Med., June 20, 2005; 201(12): 1881 - 1884.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M.-F. Pahl-Seibert, M. Juelch, J. Podlech, D. Thomas, P. Deegen, M. J. Reddehase, and R. Holtappels
Highly Protective In Vivo Function of Cytomegalovirus IE1 Epitope-Specific Memory CD8 T Cells Purified by T-Cell Receptor-Based Cell Sorting
J. Virol., May 1, 2005; 79(9): 5400 - 5413.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. Daftarian, G.-Y. Song, S. Ali, M. Faynsod, J. Longmate, D. J. Diamond, and J. D. I. Ellenhorn
Two Distinct Pathways of Immuno-Modulation Improve Potency of p53 Immunization in Rejecting Established Tumors
Cancer Res., August 1, 2004; 64(15): 5407 - 5414.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Z. Wang, C. La Rosa, S. Mekhoubad, S. F. Lacey, M. C. Villacres, S. Markel, J. Longmate, J. D. I. Ellenhorn, R. F. Siliciano, C. Buck, et al.
Attenuated poxviruses generate clinically relevant frequencies of CMV-specific T cells
Blood, August 1, 2004; 104(3): 847 - 856.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.