| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Activation and Host Defense in Pseudomonas Pneumonia1
* Department of Veterans Affairs and Division of Allergy, Pulmonary and Critical Care,
Department of Cancer Biology,
Division of Infectious Diseases, and
Department of Biomedical Engineering, Vanderbilt University School of Medicine, Nashville, TN 37232;
¶ Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305; and
|| Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892
We examined the role of redox signaling generated by NADPH oxidase in activation of NF-
B and host defense against Pseudomonas aeruginosa pneumonia. Using mice with an NF-B-driven luciferase reporter construct (HIV-LTR/luciferase (HLL)), we found that intratracheal administration of P. aeruginosa resulted in a dose-dependent neutrophilic influx and activation of NF-B. To determine the effects of reactive oxygen species generated by the NADPH oxidase system on activation of NF-B, we crossbred mice deficient in p47phox with NF-B reporter mice (p47phox-/-HLL). These p47phox-/-HLL mice were unable to activate NF-B to the same degree as HLL mice with intact NADPH oxidase following P. aeruginosa infection. In addition, lung TNF-
levels were significantly lower in p47phox-/-HLL mice compared with HLL mice. Bacterial clearance was impaired in p47phox-/-HLL mice. In vitro studies using bone marrow-derived macrophages showed that Toll-like receptor 4 was necessary for NF-B activation following treatment with P. aeruginosa. Additional studies with macrophages from p47phox-/- mice confirmed that redox signaling was necessary for maximal Toll-like receptor 4-dependent NF-B activation in this model. These data indicate that the NADPH oxidase-dependent respiratory burst stimulated by Pseudomonas infection contributes to host defense by modulating redox-dependent signaling through the NF-B pathway.
This article has been cited by other articles:
|
|
 |
|
  V. M. Weis Cellular mechanisms of Cnidarian bleaching: stress causes the collapse of symbiosis J. Exp. Biol., October 1, 2008; 211(19): 3059 - 3066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, X. Li, A. Carpinteiro, and E. Gulbins Acid Sphingomyelinase Amplifies Redox Signaling in Pseudomonas aeruginosa-Induced Macrophage Apoptosis J. Immunol., September 15, 2008; 181(6): 4247 - 4254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Marriott, L. E. Jackson, T. S. Wilkinson, A. J. Simpson, T. J. Mitchell, D. J. Buttle, S. S. Cross, P. G. Ince, P. G. Hellewell, M. K. B. Whyte, et al. Reactive Oxygen Species Regulate Neutrophil Recruitment and Survival in Pneumococcal Pneumonia Am. J. Respir. Crit. Care Med., April 15, 2008; 177(8): 887 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. T. Stathopoulos, T. P. Sherrill, W. Han, R. T. Sadikot, F. E. Yull, T. S. Blackwell, and B. Fingleton Host Nuclear Factor-{kappa}B Activation Potentiates Lung Cancer Metastasis Mol. Cancer Res., March 1, 2008; 6(3): 364 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Mizgerd and S. J. Skerrett Animal models of human pneumonia Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L387 - L398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Joo, M. Kwon, R. T. Sadikot, P. J. Kingsley, L. J. Marnett, T. S. Blackwell, R. S. Peebles Jr, Y. Urade, and J. W. Christman Induction and Function of Lipocalin Prostaglandin D Synthase in Host Immunity J. Immunol., August 15, 2007; 179(4): 2565 - 2575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Mathew, G. Y. Park, H. Cao, A. C. Azim, X. Wang, R. B. Van Breemen, R. T. Sadikot, and J. W. Christman Inhibitory {kappa}B Kinase 2 Activates Airway Epithelial Cells to Stimulate Bone Marrow Macrophages Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 562 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Koff, M. X. G. Shao, S. Kim, I. F. Ueki, and J. A. Nadel Pseudomonas Lipopolysaccharide Accelerates Wound Repair via Activation of a Novel Epithelial Cell Signaling Cascade J. Immunol., December 15, 2006; 177(12): 8693 - 8700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Vogel, J. Orrington-Myers, M. Broman, and A. B. Malik De novo ICAM-1 synthesis in the mouse lung: model of assessment of protein expression in lungs Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L496 - L501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Dehus, T. Hartung, and C. Hermann Endotoxin evaluation of eleven lipopolysaccharides by whole blood assay does not always correlate with Limulus amebocyte lysate assay Innate Immunity, June 1, 2006; 12(3): 171 - 180. [Abstract] [PDF]
|
|
|
|
|
|
V. Sancho-Shimizu and D. Malo Sequencing, Expression, and Functional Analyses Support the Candidacy of Ncf2 in Susceptibility to Salmonella Typhimurium Infection in Wild-Derived Mice. J. Immunol., June 1, 2006; 176(11): 6954 - 6961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Everhart, W. Han, T. P. Sherrill, M. Arutiunov, V. V. Polosukhin, J. R. Burke, R. T. Sadikot, J. W. Christman, F. E. Yull, and T. S. Blackwell Duration and Intensity of NF-{kappa}B Activity Determine the Severity of Endotoxin-Induced Acute Lung Injury. J. Immunol., April 15, 2006; 176(8): 4995 - 5005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Sadikot and T. S. Blackwell Bioluminescence Imaging Proceedings of the ATS, December 1, 2005; 2(6): 537 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Siore, R. E. Parker, A. A. Stecenko, C. Cuppels, M. McKean, B. W. Christman, R. Cruz-Gervis, and K. L. Brigham Endotoxin-induced acute lung injury requires interaction with the liver Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L769 - L776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Musiek, L. Gao, G. L. Milne, W. Han, M. B. Everhart, D. Wang, M. G. Backlund, R. N. DuBois, G. Zanoni, G. Vidari, et al. Cyclopentenone Isoprostanes Inhibit the Inflammatory Response in Macrophages J. Biol. Chem., October 21, 2005; 280(42): 35562 - 35570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Mora, J. LaVoy, M. McKean, A. Stecenko, K. L. Brigham, R. Parker, and M. Rojas Prevention of NF-{kappa}B activation in vivo by a cell-permeable NF-{kappa}B inhibitor peptide Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L536 - L544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Sadikot, T. S. Blackwell, J. W. Christman, and A. S. Prince Pathogen-Host Interactions in Pseudomonas aeruginosa Pneumonia Am. J. Respir. Crit. Care Med., June 1, 2005; 171(11): 1209 - 1223. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
