| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Promotes Fas Ligand- and Perforin-Mediated Liver Cell Destruction by Cytotoxic CD8 T Cells1
Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Freiburg, Germany
To study liver cell damage by CTL, CD8 T cells from P14 TCR transgenic (tg) mice specific for the gp33 epitope of lymphocytic choriomeningitis virus with either deficiency in IFN-
(P14.IFN-°), functional Fas ligand (P14.gld), or perforin (P14.PKO) were transferred into H8 tg mice ubiquitously expressing gp33 Ag. Treatment of H8 recipient mice with agonistic anti-CD40 Abs induced vigorous expansion of the transferred P14 T cells and led to liver cell destruction determined by increase of glutamate dehydrogenase serum levels and induction of caspase-3 in hepatocytes. Liver injury was mediated by the Fas/Fas ligand (FasL) pathway and by perforin, because P14.gld and P14.PKO T cells failed to induce increased glutamate dehydrogenase levels despite strong in vivo proliferation. In addition, H8 tg mice lacking Fas were resistant to the pathogenic effect of P14 T cells. Besides FasL and perforin, IFN- was also required for liver cell damage, because P14.IFN-° T cells adoptively transferred into H8 mice failed to induce disease. Moreover, Fas expression on hepatocytes from H8 recipient mice was increased after transfer of wild-type compared with P14.IFN-° T cells, and wild-type P14 T cells expressed higher levels of FasL than P14 T cells lacking IFN-. Thus, our data suggest that IFN- released by activated CD8 T cells upon Ag contact facilitates liver cell destruction.
This article has been cited by other articles:
|
|
 |
|
  H. L. Stevenson, E. C. Crossley, N. Thirumalapura, D. H. Walker, and N. Ismail Regulatory Roles of CD1d-Restricted NKT Cells in the Induction of Toxic Shock-Like Syndrome in an Animal Model of Fatal Ehrlichiosis Infect. Immun., April 1, 2008; 76(4): 1434 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Otahal, B. B. Knowles, S. S. Tevethia, and T. D. Schell Anti-CD40 Conditioning Enhances the TCD8 Response to a Highly Tolerogenic Epitope and Subsequent Immunotherapy of Simian Virus 40 T Antigen-Induced Pancreatic Tumors J. Immunol., November 15, 2007; 179(10): 6686 - 6695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Keino, S. Masli, S. Sasaki, J. W. Streilein, and J. Stein-Streilein CD8+ T Regulatory Cells Use a Novel Genetic Program that Includes CD103 to Suppress Th1 Immunity in Eye-Derived Tolerance. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1533 - 1542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Wesche-Soldato, J. L. Lomas-Neira, M. Perl, L. Jones, C.-S. Chung, and A. Ayala The role and regulation of apoptosis in sepsis Innate Immunity, December 1, 2005; 11(6): 375 - 382. [Abstract] [PDF]
|
|
|
|
|
|
J. K. Ahn, H. Chung, D.-s. Lee, Y. S. Yu, and H. G. Yu CD8brightCD56+ T Cells Are Cytotoxic Effectors in Patients with Active Behcet's Uveitis J. Immunol., November 1, 2005; 175(9): 6133 - 6142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Wesche, J. L. Lomas-Neira, M. Perl, C.-S. Chung, and A. Ayala Leukocyte apoptosis and its significance in sepsis and shock J. Leukoc. Biol., August 1, 2005; 78(2): 325 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Ahn, J.-M. Seo, J. Yu, F. S. Oh, H. Chung, and H. G. Yu Down-Regulation of IFN-{gamma}-Producing CD56+ T Cells after Combined Low-Dose Cyclosporine/Prednisone Treatment in Patients with Behcet's Uveitis Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2458 - 2464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Yarovinsky, M. P. Mohning, M. A. Bradford, M. M. Monick, and G. W. Hunninghake Increased Sensitivity to Staphylococcal Enterotoxin B following Adenoviral Infection Infect. Immun., June 1, 2005; 73(6): 3375 - 3384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Morris, A. Tsallios, G. M. Bendle, S.-a. Xue, and H. J. Stauss A critical role of T cell antigen receptor-transduced MHC class I-restricted helper T cells in tumor protection PNAS, May 31, 2005; 102(22): 7934 - 7939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Arens, P. A. Baars, M. Jak, K. Tesselaar, M. van der Valk, M. H. J. van Oers, and R. A. W. van Lier Cutting Edge: CD95 Maintains Effector T Cell Homeostasis in Chronic Immune Activation J. Immunol., May 15, 2005; 174(10): 5915 - 5920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Unsoeld and H. Pircher Complex Memory T-Cell Phenotypes Revealed by Coexpression of CD62L and CCR7 J. Virol., April 1, 2005; 79(7): 4510 - 4513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Puliaev, P. Nguyen, F. D. Finkelman, and C. S. Via Differential Requirement for IFN-{gamma} in CTL Maturation in Acute Murine Graft-versus-Host Disease J. Immunol., July 15, 2004; 173(2): 910 - 919. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
