| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany
Covalent linkage of immunostimulatory CpG-DNA to OVA (CpG-OVA complex) results in CpG-DNA-aided cross-presentation of OVA by dendritic cells (DCs). In this study, we analyzed the thesis that CpG-OVA complexes may be cross-presented by B cells to route internalized Ag into the class I MHC presentation pathway. First, we describe that conjugation of CpG-DNA to OVA enhances up to 40-fold internalization of OVA by B cells, which in turn generate the CD8 T cell epitope SIINFEKL complexed to MHC class I, albeit less efficiently than DCs. Furthermore, upon internalization, CpG-DNA conjugated to OVA stimulates B cells to up-regulate costimulatory molecules and cytokines including IL-12. Adoptive transfer of CpG-OVA complex-loaded wild-type B cells cross-primes naive CD8 T cells both in wild-type mice and in MyD88-deficient mice. Overall, these findings disclose attributes of B cells, including cross-presentation of exogenous Ag and cross-priming of naive CD8 T cells that hitherto have been considered as hallmarks restricted to DCs.
This article has been cited by other articles:
|
|
 |
|
  J. W. Wells, C. J. Cowled, F. Farzaneh, and A. Noble Combined Triggering of Dendritic Cell Receptors Results in Synergistic Activation and Potent Cytotoxic Immunity J. Immunol., September 1, 2008; 181(5): 3422 - 3431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Hoft, C. S. Eickhoff, O. K. Giddings, J. R. C. Vasconcelos, and M. M. Rodrigues Trans-Sialidase Recombinant Protein Mixed with CpG Motif-Containing Oligodeoxynucleotide Induces Protective Mucosal and Systemic Trypanosoma cruzi Immunity Involving CD8+ CTL and B Cell-Mediated Cross-Priming J. Immunol., November 15, 2007; 179(10): 6889 - 6900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bendz, S. C. Ruhland, M. J. Pandya, O. Hainzl, S. Riegelsberger, C. Brauchle, M. P. Mayer, J. Buchner, R. D. Issels, and E. Noessner Human Heat Shock Protein 70 Enhances Tumor Antigen Presentation through Complex Formation and Intracellular Antigen Delivery without Innate Immune Signaling J. Biol. Chem., October 26, 2007; 282(43): 31688 - 31702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Menard, L. A. Minns, S. Darche, D. W. Mielcarz, D. M. Foureau, D. Roos, F. Dzierszinski, L. H. Kasper, and D. Buzoni-Gatel B Cells Amplify IFN-{gamma} Production By T Cells via a TNF-{alpha}-Mediated Mechanism J. Immunol., October 1, 2007; 179(7): 4857 - 4866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Spinner, R. B. Kascsak, G. LaFauci, H. C. Meeker, X. Ye, M. J. Flory, J. I. Kim, G. B. Schuller-Levis, W. R. Levis, T. Wisniewski, et al. CpG oligodeoxynucleotide-enhanced humoral immune response and production of antibodies to prion protein PrPSc in mice immunized with 139A scrapie-associated fibrils J. Leukoc. Biol., June 1, 2007; 81(6): 1374 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Valmori, N. E. Souleimanian, V. Tosello, N. Bhardwaj, S. Adams, D. O'Neill, A. Pavlick, J. B. Escalon, C. M. Cruz, A. Angiulli, et al. Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming PNAS, May 22, 2007; 104(21): 8947 - 8952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Pawar, P. S. Patole, M. Wornle, and H.-J. Anders Microbial nucleic acids pay a Toll in kidney disease Am J Physiol Renal Physiol, September 1, 2006; 291(3): F509 - F516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chung, B.-S. Kim, Y.-J. Kim, H.-J. Ko, S.-Y. Ko, D.-H. Kim, and C.-Y. Kang CD1d-Restricted T Cells License B Cells to Generate Long-Lasting Cytotoxic Antitumor Immunity In vivo. Cancer Res., July 1, 2006; 66(13): 6843 - 6850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lauterbach, A. Gruber, C. Ried, C. Cheminay, and T. Brocker Insufficient APC Capacities of Dendritic Cells in Gene Gun-Mediated DNA Vaccination. J. Immunol., April 15, 2006; 176(8): 4600 - 4607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Martinez, M. Vermeulen, A. Trevani, A. Ceballos, J. Sabatte, R. Gamberale, M. E. Alvarez, G. Salamone, T. Tanos, O. A. Coso, et al. Extracellular Acidosis Induces Neutrophil Activation by a Mechanism Dependent on Activation of Phosphatidylinositol 3-Kinase/Akt and ERK Pathways J. Immunol., January 15, 2006; 176(2): 1163 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. S. Araujo, B. C. G. de Alencar, J. R. C. Vasconcelos, M. I. Hiyane, C. R. F. Marinho, M. L. O. Penido, S. B. Boscardin, D. F. Hoft, R. T. Gazzinelli, and M. M. Rodrigues CD8+-T-Cell-Dependent Control of Trypanosoma cruzi Infection in a Highly Susceptible Mouse Strain after Immunization with Recombinant Proteins Based on Amastigote Surface Protein 2 Infect. Immun., September 1, 2005; 73(9): 6017 - 6025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. R. Tobian, C. V. Harding, and D. H. Canaday Mycobacterium tuberculosis Heat Shock Fusion Protein Enhances Class I MHC Cross-Processing and -Presentation by B Lymphocytes J. Immunol., May 1, 2005; 174(9): 5209 - 5214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hon, A. Oran, T. Brocker, and J. Jacob B Lymphocytes Participate in Cross-Presentation of Antigen following Gene Gun Vaccination J. Immunol., May 1, 2005; 174(9): 5233 - 5242. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
