| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720
To gain insight into NK cell dynamics, we investigated the turnover and proliferation rates of NK cells in normal and lymphopenic conditions. In contrast to previous reports suggesting a very rapid turnover of NK cells, continuous 5-bromo-2'-deoxyuridine (BrdU)-labeling studies demonstrated that the time necessary for labeling 50% of splenic NK cells in mature mice was 17 days, similar to the rate of labeling of memory T cells. In contrast, in young mice, splenic NK cells labeled very rapidly with BrdU, although cell cycle analyses and BrdU pulse-labeling studies suggested that most of this proliferation occurred in a precursor population. A somewhat larger percentage of bone marrow NK cells was cycling, suggesting that these proliferating cells are the precursors of the mostly nondividing or slowly dividing splenic NK cells. Splenic NK cells from mature mice also did not proliferate significantly when transferred to normal mice, but did proliferate when transferred to irradiated mice. Thus, NK cells, like T cells, undergo homeostatic proliferation in a lymphopenic environment. Homeostatic proliferation of NK cells was not dependent on host cell class I molecules or host production of IL-15. Nevertheless, the number of recovered NK cells was much lower in IL-15-/- hosts. These results suggest that IL-15 is not essential for homeostatic proliferation of NK cells, but is necessary for survival of the NK cells. Our results provide important basic information concerning the production and replacement of NK cells.
This article has been cited by other articles:
|
|
 |
|
  L. Chiossone, J. Chaix, N. Fuseri, C. Roth, E. Vivier, and T. Walzer Maturation of mouse NK cells is a 4-stage developmental program Blood, May 28, 2009; 113(22): 5488 - 5496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. French, C. L. Roark, W. K. Born, and R. L. O'Brien {gamma}{delta} T Lymphocyte Homeostasis Is Negatively Regulated by {beta}2-Microglobulin J. Immunol., February 15, 2009; 182(4): 1892 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. H. E. Mabarrack, N. L. Turner, and G. Mayrhofer Recent thymic origin, differentiation, and turnover of regulatory T cells J. Leukoc. Biol., November 1, 2008; 84(5): 1287 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Jay, L. M. Reed-Loisel, and P. E. Jensen Polyclonal MHC Ib-Restricted CD8+ T Cells Undergo Homeostatic Expansion in the Absence of Conventional MHC-Restricted T Cells J. Immunol., March 1, 2008; 180(5): 2805 - 2814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang and G. G. Meadows Chronic alcohol consumption perturbs the balance between thymus-derived and bone marrow-derived natural killer cells in the spleen J. Leukoc. Biol., January 1, 2008; 83(1): 41 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Taniguchi, D. Guzior, and V. Kumar 2B4 inhibits NK-cell fratricide Blood, September 15, 2007; 110(6): 2020 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Huntington, H. Tabarias, K. Fairfax, J. Brady, Y. Hayakawa, M. A. Degli-Esposti, M. J. Smyth, D. M. Tarlinton, and S. L. Nutt NK Cell Maturation and Peripheral Homeostasis Is Associated with KLRG1 Up-Regulation J. Immunol., April 15, 2007; 178(8): 4764 - 4770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. van Zelm, T. Szczepanski, M. van der Burg, and J. J.M. van Dongen Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion J. Exp. Med., March 19, 2007; 204(3): 645 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Walzer, M. Blery, J. Chaix, N. Fuseri, L. Chasson, S. H. Robbins, S. Jaeger, P. Andre, L. Gauthier, L. Daniel, et al. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46 PNAS, February 27, 2007; 104(9): 3384 - 3389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Regunathan, Y. Chen, S. Kutlesa, X. Dai, L. Bai, R. Wen, D. Wang, and S. Malarkannan Differential and Nonredundant Roles of Phospholipase C{gamma}2 and Phospholipase C{gamma}1 in the Terminal Maturation of NK Cells J. Immunol., October 15, 2006; 177(8): 5365 - 5376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. McGrath-Morrow, B. Laube, S.-C. Tzou, C. Cho, J. Cleary, H. Kimura, N. R. Rose, and P. Caturegli IL-12 overexpression in mice as a model for Sjogren lung disease Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L837 - L846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cappuccio, M. Elishmereni, and Z. Agur Cancer immunotherapy by interleukin-21: potential treatment strategies evaluated in a mathematical model. Cancer Res., July 15, 2006; 66(14): 7293 - 7300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Tato, N. Mason, D. Artis, S. Shapira, J. C. Caamano, J. H. Bream, H.-C. Liou, and C. A. Hunter Opposing roles of NF-{kappa}B family members in the regulation of NK cell proliferation and production of IFN-{gamma} Int. Immunol., April 1, 2006; 18(4): 505 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chiesa, M. Mingueneau, N. Fuseri, B. Malissen, D. H. Raulet, M. Malissen, E. Vivier, and E. Tomasello Multiplicity and plasticity of natural killer cell signaling pathways Blood, March 15, 2006; 107(6): 2364 - 2372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hayakawa and M. J. Smyth CD27 Dissects Mature NK Cells into Two Subsets with Distinct Responsiveness and Migratory Capacity J. Immunol., February 1, 2006; 176(3): 1517 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Y. Setiady, P. Pramoonjago, and K. S. K. Tung Requirements of NK Cells and Proinflammatory Cytokines in T Cell-Dependent Neonatal Autoimmune Ovarian Disease Triggered by Immune Complex J. Immunol., July 15, 2004; 173(2): 1051 - 1058. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
