HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taylor, P. R. Articles by Gordon, S. Search for Related Content PubMed PubMed Citation Articles by Taylor, P. R. Articles by Gordon, S.

The Role of SIGNR1 and the -Glucan Receptor (Dectin-1) in the Nonopsonic Recognition of Yeast by Specific Macrophages¹

Philip R. Taylor^2,*, Gordon D. Brown^*, Jurgen Herre^*, David L. Williams, Janet A. Willment^* and Siamon Gordon^*

^* Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford, United Kingdom; and Department of Surgery, James H. Quillen College of Medicine, Johnson City, TN 37614

We recently demonstrated that the -glucan receptor Dectin-1 (GR) was the major nonopsonic -glucan receptor on macrophages (M) for the yeast-derived particle zymosan. However, on resident peritoneal M, we identified an additional mannan-inhibitable receptor for zymosan that was distinct from the M mannose receptor (MR). In this study, we have studied the mannose-binding potential of murine M and identified the dendritic cell-specific ICAM-3-grabbing nonintegrin homolog, SIGN-related 1 (SIGNR1), as a major MR on murine resident peritoneal M. Both SIGNR1 and GR cooperated in the nonopsonic recognition of zymosan by these M. When SIGNR1 was introduced into NIH3T3 fibroblasts or RAW 264.7 M, it conferred marked zymosan-binding potential on these cells. However, in the nonprofessional phagocytes (NIH3T3), SIGNR1 was found to be poorly phagocytic, suggesting that other receptors such as GR may play a more dominant role in particle internalization on professional phagocytes. Binding of zymosan to RAW 264.7 M expressing SIGNR1 resulted in TNF- production. Treatment of RAW 264.7 M expressing SIGNR1, which express low levels of GR, with -glucans had little effect on binding or TNF- production, indicating that there was no absolute requirement for GR in this process. These studies have identified SIGNR1 as a major MR for fungal and other pathogens present on specific subsets of M.

This article has been cited by other articles:

				M. Rosas, K. Liddiard, M. Kimberg, I. Faro-Trindade, J. U. McDonald, D. L. Williams, G. D. Brown, and P. R. Taylor The Induction of Inflammation by Dectin-1 In Vivo Is Dependent on Myeloid Cell Programming and the Progression of Phagocytosis J. Immunol., September 1, 2008; 181(5): 3549 - 3557. [Abstract] [Full Text] [PDF]

				H. Sahly, Y. Keisari, E. Crouch, N. Sharon, and I. Ofek Recognition of Bacterial Surface Polysaccharides by Lectins of the Innate Immune System and Its Contribution to Defense against Infection: the Case of Pulmonary Pathogens Infect. Immun., April 1, 2008; 76(4): 1322 - 1332. [Full Text] [PDF]

				A. Koneti, M. J. Linke, E. Brummer, and D. A. Stevens Evasion of Innate Immune Responses: Evidence for Mannose Binding Lectin Inhibition of Tumor Necrosis Factor Alpha Production by Macrophages in Response to Blastomyces dermatitidis Infect. Immun., March 1, 2008; 76(3): 994 - 1002. [Abstract] [Full Text] [PDF]

				A. G. Rothfuchs, A. Bafica, C. G. Feng, J. G. Egen, D. L. Williams, G. D. Brown, and A. Sher Dectin-1 Interaction with Mycobacterium tuberculosis Leads to Enhanced IL-12p40 Production by Splenic Dendritic Cells J. Immunol., September 15, 2007; 179(6): 3463 - 3471. [Abstract] [Full Text] [PDF]

				E. Doz, S. Rose, J. Nigou, M. Gilleron, G. Puzo, F. Erard, B. Ryffel, and V. F. J. Quesniaux Acylation Determines the Toll-like receptor (TLR)-dependent Positive Versus TLR2-, Mannose Receptor-, and SIGNR1-independent Negative Regulation of Pro-inflammatory Cytokines by Mycobacterial Lipomannan J. Biol. Chem., September 7, 2007; 282(36): 26014 - 26025. [Abstract] [Full Text] [PDF]

				T. Jouault, M. El Abed-El Behi, M. Martinez-Esparza, L. Breuilh, P.-A. Trinel, M. Chamaillard, F. Trottein, and D. Poulain Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling J. Immunol., October 1, 2006; 177(7): 4679 - 4687. [Abstract] [Full Text] [PDF]

				E. P. McGreal, M. Rosas, G. D. Brown, S. Zamze, S. Y.C. Wong, S. Gordon, L. Martinez-Pomares, and P. R. Taylor The carbohydrate-recognition domain of Dectin-2 is a C-type lectin with specificity for high mannose Glycobiology, May 1, 2006; 16(5): 422 - 430. [Abstract] [Full Text] [PDF]

				S. E. M. Heinsbroek, P. R. Taylor, M. Rosas, J. A. Willment, D. L. Williams, S. Gordon, and G. D. Brown Expression of Functionally Different Dectin-1 Isoforms by Murine Macrophages J. Immunol., May 1, 2006; 176(9): 5513 - 5518. [Abstract] [Full Text] [PDF]

				S. Suram, G. D. Brown, M. Ghosh, S. Gordon, R. Loper, P. R. Taylor, S. Akira, S. Uematsu, D. L. Williams, and C. C. Leslie Regulation of Cytosolic Phospholipase A2 Activation and Cyclooxygenase 2 Expression in Macrophages by the beta-Glucan Receptor J. Biol. Chem., March 3, 2006; 281(9): 5506 - 5514. [Abstract] [Full Text] [PDF]

				K. Nagaoka, K. Takahara, K. Tanaka, H. Yoshida, R. M. Steinman, S.-i. Saitoh, S. Akashi-Takamura, K. Miyake, Y. S. Kang, C. G. Park, et al. Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria Int. Immunol., July 1, 2005; 17(7): 827 - 836. [Abstract] [Full Text] [PDF]

				N. Fernandez, S. Alonso, I. Valera, A. G. Vigo, M. Renedo, L. Barbolla, and M. S. Crespo Mannose-Containing Molecular Patterns Are Strong Inducers of Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Human Macrophages J. Immunol., June 15, 2005; 174(12): 8154 - 8162. [Abstract] [Full Text] [PDF]

				G. de la Rosa, M. Yanez-Mo, R. Samaneigo, D. Serrano-Gomez, L. Martinez-Munoz, E. Fernandez-Ruiz, N. Longo, F. Sanchez-Madrid, A. L. Corbi, and P. Sanchez-Mateos Regulated recruitment of DC-SIGN to cell-cell contact regions during zymosan-induced human dendritic cell aggregation J. Leukoc. Biol., May 1, 2005; 77(5): 699 - 709. [Abstract] [Full Text] [PDF]

				J. Herre, A. S. J. Marshall, E. Caron, A. D. Edwards, D. L. Williams, E. Schweighoffer, V. Tybulewicz, C. R. e Sousa, S. Gordon, and G. D. Brown Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages Blood, December 15, 2004; 104(13): 4038 - 4045. [Abstract] [Full Text] [PDF]

				A. Lanoue, M. R. Clatworthy, P. Smith, S. Green, M. J. Townsend, H. E. Jolin, K. G.C. Smith, P. G. Fallon, and A. N.J. McKenzie SIGN-R1 Contributes to Protection against Lethal Pneumococcal Infection in Mice J. Exp. Med., December 6, 2004; 200(11): 1383 - 1393. [Abstract] [Full Text] [PDF]

				R. P. Hobson, C. A. Munro, S. Bates, D. M. MacCallum, J. E. Cutler, S. E. M. Heinsbroek, G. D. Brown, F. C. Odds, and N. A.R. Gow Loss of Cell Wall Mannosylphosphate in Candida albicans Does Not Influence Macrophage Recognition J. Biol. Chem., September 17, 2004; 279(38): 39628 - 39635. [Abstract] [Full Text] [PDF]

				K. Takahara, Y. Yashima, Y. Omatsu, H. Yoshida, Y. Kimura, Y.-S. Kang, R. M. Steinman, C. G. Park, and K. Inaba Functional comparison of the mouse DC-SIGN, SIGNR1, SIGNR3 and Langerin, C-type lectins Int. Immunol., June 1, 2004; 16(6): 819 - 829. [Abstract] [Full Text] [PDF]