The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cronshaw, D. G.
Right arrow Articles by Ward, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cronshaw, D. G.
Right arrow Articles by Ward, S. G.
The Journal of Immunology, 2004, 172: 7761-7770.
Copyright © 2004 by The American Association of Immunologists

Activation of Phosphoinositide 3-Kinases by the CCR4 Ligand Macrophage-Derived Chemokine Is a Dispensable Signal for T Lymphocyte Chemotaxis1

Darran G. Cronshaw*, Charles Owen{dagger}, Zarin Brown{dagger} and Stephen G. Ward2,*

* Department of Pharmacy and Pharmacology, University of Bath, Bath, United Kingdom; and {dagger} Novartis Horsham Research Center, Horsham, United Kingdom

Macrophage-derived chemokine (MDC/CC chemokine ligand 22 (CCL22)) mediates its cellular effects principally by binding to its receptor CCR4, and together they constitute a multifunctional chemokine/receptor system with homeostatic and inflammatory roles in the body. We report the CCL22-induced accumulation of phosphatidylinositol-(3,4,5)-trisphosphate (PI(3,4,5)P3) in the leukemic T cell line CEM. CCL22 also had the ability to chemoattract human Th2 cells and CEM cells in a pertussis toxin-sensitive manner. Although the PI(3,4,5)P3 accumulation along with the pertussis toxin-susceptible phosphorylation of protein kinase B were sensitive to the two phosphoinositide 3-kinase inhibitors, LY294002 and wortmannin, cell migration was unaffected. However, cell migration was abrogated with the Rho-dependent kinase inhibitor, Y-27632. These data demonstrate that although there is PI(3,4,5)P3 accumulation downstream of CCR4, phosphoinositide 3-kinase activity is a dispensable signal for CCR4-stimulated chemotaxis of Th2 cells and the CEM T cell line.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. Webb, A. Johnson, M. Fortunato, A. Platt, T. Crabbe, M. I. Christie, G. F. Watt, S. G. Ward, and L. A. Jopling
Evidence for PI-3K-dependent migration of Th17-polarized cells in response to CCR2 and CCR6 agonists
J. Leukoc. Biol., October 1, 2008; 84(4): 1202 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
K. M. Zaffuto, M. E. Piccone, T. G. Burrage, C. A. Balinsky, G. R. Risatti, M. V. Borca, L. G. Holinka, D. L. Rock, and C. L. Afonso
Classical swine fever virus inhibits nitric oxide production in infected macrophages
J. Gen. Virol., November 1, 2007; 88(11): 3007 - 3012.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Park, I. Park, D. Lee, Y. B. Choi, H. Lee, and Y. Yun
The adaptor protein Lad associates with the G protein {beta} subunit and mediates chemokine-dependent T-cell migration
Blood, June 15, 2007; 109(12): 5122 - 5128.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. G. Cronshaw, A. Kouroumalis, R. Parry, A. Webb, Z. Brown, and S. G. Ward
Evidence that phospholipase C-dependent, calcium-independent mechanisms are required for directional migration of T lymphocytes in response to the CCR4 ligands CCL17 and CCL22
J. Leukoc. Biol., June 1, 2006; 79(6): 1369 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Bagchi, Z. Liao, F. A. Gonzalez, N. E. Chorna, C. I. Seye, G. A. Weisman, and L. Erb
The P2Y2 Nucleotide Receptor Interacts with {alpha}v Integrins to Activate Go and Induce Cell Migration
J. Biol. Chem., November 25, 2005; 280(47): 39050 - 39057.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Kouroumalis, R. J. Nibbs, H. Aptel, K. L. Wright, G. Kolios, and S. G. Ward
The Chemokines CXCL9, CXCL10, and CXCL11 Differentially Stimulate G{alpha}i-Independent Signaling and Actin Responses in Human Intestinal Myofibroblasts
J. Immunol., October 15, 2005; 175(8): 5403 - 5411.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Gao, R. L. Wange, N. Zhang, J. J. Oppenheim, and O. M. Z. Howard
Negative regulation of CXCR4-mediated chemotaxis by the lipid phosphatase activity of tumor suppressor PTEN
Blood, October 15, 2005; 106(8): 2619 - 2626.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Riol-Blanco, N. Sanchez-Sanchez, A. Torres, A. Tejedor, S. Narumiya, A. L. Corbi, P. Sanchez-Mateos, and J. L. Rodriguez-Fernandez
The Chemokine Receptor CCR7 Activates in Dendritic Cells Two Signaling Modules That Independently Regulate Chemotaxis and Migratory Speed
J. Immunol., April 1, 2005; 174(7): 4070 - 4080.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. A. Lacalle, C. Gomez-Mouton, D. F. Barber, S. Jimenez-Baranda, E. Mira, C. Martinez-A., A. C. Carrera, and S. Manes
PTEN regulates motility but not directionality during leukocyte chemotaxis
J. Cell Sci., December 1, 2004; 117(25): 6207 - 6215.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.