| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Receptor Activation by Activated T Cells Induces Cytokine Release from Mouse Bone Marrow-Derived Mast Cells1
Department of Immunology, University of Regensburg, Regensburg, Germany
Lymphotoxin-
receptor (LTR) signaling is known to play a key role in embryonic lymphoid organ formation as well as maintenance of lymphoid architecture. Activation of the LTR is induced by either the heterotrimeric lymphotoxin-
12 (LT12) or the homotrimeric LIGHT (homologous to lymphotoxins, exhibits inducible expression, and competes with HSV gpD for herpes virus entry mediator, a receptor expressed by T lymphocyte). Both ligands are expressed on activated lymphocytes. As mast cells reside in close proximity to activated T cells in some inflammatory tissues, we examined the expression of LTR on bone marrow-derived mast cells and asked whether the LTR-ligand interaction would allow communication between mast cells and activated T cells. We found that mast cells express LTR at the mRNA as well as at the protein level. To investigate LTR-specific mast cell activation, the LTR on BMMC from either wild-type or LTR-deficient mice was stimulated with recombinant mouse LIGHT or agonistic mAbs in the presence of ionomycin. LTR-specific release of the cytokines IL-4, IL-6, TNF, and the chemokines macrophage inflammatory protein 2 and RANTES was detected. Moreover, coculture of mast cells with T cells expressing the LTR ligands also entailed the release of these cytokines. Interference with a specific LTR inhibitor resulted in significant suppression of mast cell cytokine release. These data clearly show that LTR expressed on mast cells can transduce a costimulatory signal in T cell-dependent mast cell activation.
This article has been cited by other articles:
|
|
 |
|
  R. Grabner, K. Lotzer, S. Dopping, M. Hildner, D. Radke, M. Beer, R. Spanbroek, B. Lippert, C. A. Reardon, G. S. Getz, et al. Lymphotoxin {beta} receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice J. Exp. Med., January 16, 2009; 206(1): 233 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Kang, K. A. Kudsk, Y. Sano, J. Lan, F. Yang-Xin, F. E. Gomez, and Y. Maeshima Effects of Lymphotoxin {beta} Receptor Blockade on Intestinal Mucosal Immunity JPEN J Parenter Enteral Nutr, September 1, 2007; 31(5): 358 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Moore, S. Buonocore, E. Aksoy, N. Ouled-Haddou, S. Goriely, E. Lazarova, F. Paulart, C. Heirman, E. Vaeremans, K. Thielemans, et al. An Alternative Pathway of NF-{kappa}B Activation Results in Maturation and T Cell Priming Activity of Dendritic Cells Overexpressing a Mutated I{kappa}B{alpha} J. Immunol., February 1, 2007; 178(3): 1301 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Mortarini, A. Scarito, D. Nonaka, M. Zanon, I. Bersani, E. Montaldi, E. Pennacchioli, R. Patuzzo, M. Santinami, and A. Anichini Constitutive Expression and Costimulatory Function of LIGHT/TNFSF14 on Human Melanoma Cells and Melanoma-Derived Microvesicles Cancer Res., April 15, 2005; 65(8): 3428 - 3436. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
