| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Laboratories of
*
Mycobacterial Immunology,
Allergology, and
Mycobacterial Antigens, Pasteur Institute of Brussels, Brussels, Belgium;
Department of Immunohematology & Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands; and
¶
Institut de Biologie et de Médecine Moléculaire, Université Libre de Bruxelles, Gosselies, Belgium
Using plasmid vaccination with DNA encoding the putative phosphate transport receptor PstS-3 from Mycobacterium tuberculosis and 36 overlapping 20-mer peptides spanning the entire PstS-3 sequence, we determined the immunodominant Th1-type CD4+ T cell epitopes in C57BL/10 mice, as measured by spleen cell IL-2 and IFN-
production. Furthermore, a potent IFN--inducing, Db-restricted CD8+ epitope was identified using MHC class I mutant B6.C-H-2bm13 mice and intracellular IFN- and whole blood CD8+ T cell tetramer staining. Using adoptive transfer of CFSE-labeled, peptide-pulsed syngeneic spleen cells from naive animals into DNA vaccinated or M. tuberculosis-infected recipients, we demonstrated a functional in vivo CTL activity against this Db-restricted PstS-3 epitope. IFN- ELISPOT responses to this epitope were also detected in tuberculosis-infected mice. The CD4+ and CD8+ T cell epitopes defined for PstS-3 were completely specific and not recognized in mice vaccinated with either PstS-1 or PstS-2 DNA. The H-2 haplotype exerted a strong influence on immune reactivity to the PstS-3 Ag, and mice of the H-2b, p, and f haplotype produced significant Ab and Th1-type cytokine levels, whereas mice of H-2d, k, r, s, and q haplotype were completely unreactive. Low responsiveness against PstS-3 in MHC class II mutant B6.C-H-2bm12 mice could be overcome by DNA vaccination. IFN--producing CD8+ T cells could also be detected against the Db-restricted epitope in H-2p haplotype mice. These results highlight the potential of DNA vaccination for the induction and characterization of CD4+ and particularly CD8+ T cell responses against mycobacterial Ags.
This article has been cited by other articles:
|
|
 |
|
  J. S. Woodworth, Y. Wu, and S. M. Behar Mycobacterium tuberculosis-Specific CD8+ T Cells Require Perforin to Kill Target Cells and Provide Protection In Vivo J. Immunol., December 15, 2008; 181(12): 8595 - 8603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schaefer, N. Reiling, C. Fessler, J. Stephani, I. Taniuchi, F. Hatam, A. O. Yildirim, H. Fehrenbach, K. Walter, J. Ruland, et al. Decreased Pathology and Prolonged Survival of Human DC-SIGN Transgenic Mice during Mycobacterial Infection J. Immunol., May 15, 2008; 180(10): 6836 - 6845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Roupie, M. Romano, L. Zhang, H. Korf, M. Y. Lin, K. L. M. C. Franken, T. H. M. Ottenhoff, M. R. Klein, and K. Huygen Immunogenicity of Eight Dormancy Regulon-Encoded Proteins of Mycobacterium tuberculosis in DNA-Vaccinated and Tuberculosis-Infected Mice Infect. Immun., February 1, 2007; 75(2): 941 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D'Souza, M. Romano, J. Korf, X.-M. Wang, P.-Y. Adnet, and K. Huygen Partial Reconstitution of the CD4+-T-Cell Compartment in CD4 Gene Knockout Mice Restores Responses to Tuberculosis DNA Vaccines. Infect. Immun., May 1, 2006; 74(5): 2751 - 2759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Irwin, A. A. Izzo, S. W. Dow, Y. A. W. Skeiky, S. G. Reed, M. R. Alderson, and I. M. Orme Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein Infect. Immun., September 1, 2005; 73(9): 5809 - 5816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Kamath, J. Woodworth, X. Xiong, C. Taylor, Y. Weng, and S. M. Behar Cytolytic CD8+ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection J. Exp. Med., December 6, 2004; 200(11): 1479 - 1489. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
