HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Franchini, M. Articles by Suter, M. Search for Related Content PubMed PubMed Citation Articles by Franchini, M. Articles by Suter, M.

Dendritic Cells from Mice Neonatally Vaccinated with Modified Vaccinia Virus Ankara Transfer Resistance against Herpes Simplex Virus Type I to Naive One-Week-Old Mice¹

Marco Franchini^*, Hanspeter Hefti^*, Sabine Vollstedt^*, Bettina Glanzmann^*, Matthias Riesen^*, Mathias Ackermann^*, Paul Chaplin, Ken Shortman^*, and Mark Suter^2,*

^* Institute of Virology, University of Zurich, Zurich, Switzerland; Bavarian Nordic, Copenhagen, Denmark; and Walter and Eliza Hall Institute of Medical Research, Melbourne Australia

Modified vaccinia Ankara (MVA) is an attenuated virus. MVA induces the production of IFN and Flt3-L (FL), which results in the expansion of dendritic cells (DC) and enhanced resistance against viral infections. We report on the interplay among IFN, FL, and DC in the resistance against heterologous virus after injection of neonatal mice with MVA. The induction of serum FL was tested on day 2, and the expansion of DC was tested 1 wk after treatment with MVA. At this time point the resistance against infection with heterologous virus was also determined. After MVA treatment, serum FL was enhanced, and DC, including plasmacytoid cells in spleen, were increased in number. Mice that lacked functional IFN type I and II systems failed to increase both the concentration of FL and the number of DC. Treatment with MVA enhanced resistance against HSV-1 in wild-type animals 100-fold, but animals without a functional IFN system were not protected. Transfer of CD11c⁺ cells from MVA-treated mice into naive animals protected against lethal infection with HSV-1. Thus, although the increased resistance could be largely attributed to the increase in activation of IFN-producing plasmacytoid cells, this, in turn, depends on a complex interplay between the DC and T cell systems involving both FL and IFNs.

This article has been cited by other articles:

				B. Fancke, M. Suter, H. Hochrein, and M. O'Keeffe M-CSF: a novel plasmacytoid and conventional dendritic cell poietin Blood, January 1, 2008; 111(1): 150 - 159. [Abstract] [Full Text] [PDF]

				Z. Waibler, M. Anzaghe, H. Ludwig, S. Akira, S. Weiss, G. Sutter, and U. Kalinke Modified Vaccinia Virus Ankara Induces Toll-Like Receptor-Independent Type I Interferon Responses J. Virol., November 15, 2007; 81(22): 12102 - 12110. [Abstract] [Full Text] [PDF]

				K. J. Stittelaar, G. van Amerongen, I. Kondova, T. Kuiken, R. F. van Lavieren, F. H. M. Pistoor, H. G. M. Niesters, G. van Doornum, B. A. M. van der Zeijst, L. Mateo, et al. Modified Vaccinia Virus Ankara Protects Macaques against Respiratory Challenge with Monkeypox Virus J. Virol., June 15, 2005; 79(12): 7845 - 7851. [Abstract] [Full Text] [PDF]