The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kasow, K. A.
Right arrow Articles by Riberdy, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kasow, K. A.
Right arrow Articles by Riberdy, J. M.
The Journal of Immunology, 2004, 172: 6123-6128.
Copyright © 2004 by The American Association of Immunologists

Human CD4+CD25+ Regulatory T Cells Share Equally Complex and Comparable Repertoires with CD4+CD25– Counterparts 1

Kimberly A. Kasow, Xiaohua Chen, James Knowles, David Wichlan, Rupert Handgretinger and Janice M. Riberdy2

Division of Stem Cell Transplantation, St. Jude Children’s Research Hospital, Memphis, TN 38105

CD4+CD25+ T cells are critical mediators of peripheral immune tolerance. However, many developmental and functional characteristics of these cells are unknown, and knowledge of human regulatory T cells is particularly limited. To better understand how human CD4+CD25+ T cells develop and function, we examined the diversity of CD4+CD25+ and CD4+CD25– T cell repertoires in both thymus and peripheral blood. Levels of T receptor excision circles (TREC) were comparable in purified CD4+CD25+ and CD4+CD25– thymic populations, but were significantly higher than those in samples derived from peripheral blood, consistent with murine studies demonstrating thymic development of CD4+CD25+ regulatory T cells. Surprisingly, CD4+CD25– T cells isolated from peripheral blood had greater TREC quantities than their CD4+CD25+ counterparts, supporting the possibility of extrathymic expansion as well. CD4+CD25+ and CD4+CD25– T cells from a given individual showed overlapping profiles with respect to diversity by V{beta} staining and spectratyping. Interestingly, CD4+CD25+ T cells have lower quantities of CD3 than CD4+CD25– T cells. Collectively, these data suggest that human CD4+CD25+ T cells recognize a similar array of Ags as CD4+CD25– T cells. However, reduced levels of TCR on regulatory T cells suggest different requirements for activation and may contribute to how the immune system regulates whether a particular response is suppressed or augmented.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. M. Ertelt, J. H. Rowe, T. M. Johanns, J. C. Lai, J. B. McLachlan, and S. S. Way
Selective Priming and Expansion of Antigen-Specific Foxp3-CD4+ T Cells during Listeria monocytogenes Infection
J. Immunol., March 1, 2009; 182(5): 3032 - 3038.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Li, E. J. Gowans, C. Chougnet, M. Plebanski, and U. Dittmer
Natural Regulatory T Cells and Persistent Viral Infection
J. Virol., January 1, 2008; 82(1): 21 - 30.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
J.-R. Pallandre, E. Brillard, G. Crehange, A. Radlovic, J.-P. Remy-Martin, P. Saas, P.-S. Rohrlich, X. Pivot, X. Ling, P. Tiberghien, et al.
Role of STAT3 in CD4+CD25+FOXP3+ Regulatory Lymphocyte Generation: Implications in Graft-versus-Host Disease and Antitumor Immunity
J. Immunol., December 1, 2007; 179(11): 7593 - 7604.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Fazilleau, H. Bachelez, M.-L. Gougeon, and M. Viguier
Cutting Edge: Size and Diversity of CD4+CD25high Foxp3+ Regulatory T Cell Repertoire in Humans: Evidence for Similarities and Partial Overlapping with CD4+CD25 T Cells
J. Immunol., September 15, 2007; 179(6): 3412 - 3416.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
C. Fozza, E. Nadal, M. Longinotti, and F. Dazzi
T-cell receptor repertoire usage after allografting differs between CD4+CD25+ regulatory T cells and their CD4+CD25 counterpart
Haematologica, February 1, 2007; 92(2): 206 - 214.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Kekalainen, H. Tuovinen, J. Joensuu, M. Gylling, R. Franssila, N. Pontynen, K. Talvensaari, J. Perheentupa, A. Miettinen, and T. P. Arstila
A Defect of Regulatory T Cells in Patients with Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy
J. Immunol., January 15, 2007; 178(2): 1208 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Ribot, P. Romagnoli, and J. P. M. van Meerwijk
Agonist Ligands Expressed by Thymic Epithelium Enhance Positive Selection of Regulatory T Lymphocytes from Precursors with a Normally Diverse TCR Repertoire
J. Immunol., July 15, 2006; 177(2): 1101 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Beyer, M. Kochanek, T. Giese, E. Endl, M. R. Weihrauch, P. A. Knolle, S. Classen, and J. L. Schultze
In vivo peripheral expansion of naive CD4+CD25high FoxP3+ regulatory T cells in patients with multiple myeloma
Blood, May 15, 2006; 107(10): 3940 - 3949.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. M. Hanash and R. B. Levy
Donor CD4+CD25+ T cells promote engraftment and tolerance following MHC-mismatched hematopoietic cell transplantation
Blood, February 15, 2005; 105(4): 1828 - 1836.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.