| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840;
Inflammation Program, University of Iowa, and Iowa City Veterans Administration Medical Center, Coralville, IA 52241; and
Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
Human polymorphonuclear leukocytes (PMNs or neutrophils) kill invading microorganisms with reactive oxygen species (ROS) and cytotoxic granule components. PMNs from individuals with X-linked chronic granulomatous disease (XCGD) do not produce ROS, thereby rendering these individuals more susceptible to infection. In addition, XCGD patients develop tissue granulomas that obstruct vital organs, the mechanism(s) for which are unknown. To gain insight into the molecular processes that contribute to the pathophysiology of XCGD, including formation of granulomas, we compared global gene expression in PMNs from XCGD patients and healthy control individuals. Genes encoding mediators of inflammation and host defense, including CD11c, CD14, CD54, Fc
R1, Fc
R, CD120b, TLR5, IL-4R, CCR1, p47phox, p40phox, IL-8, CXCL1, Nramp1, and calgranulins A and B, were up-regulated constitutively in unstimulated XCGD patient PMNs. By comparing transcript levels in normal and XCGD PMNs after phagocytosis, we discovered 206 genes whose expression changed in the presence and the absence of ROS, respectively. Notably, altered Bcl2-associated X protein synthesis accompanied defective neutrophil apoptosis in XCGD patients. We hypothesize that granuloma formation in XCGD patients reflects both increased proinflammatory activity and defective PMN apoptosis, and we conclude that ROS contribute directly or indirectly to the resolution of the inflammatory response by influencing PMN gene transcription.
This article has been cited by other articles:
|
|
 |
|
  J. Huang, V. Canadien, G. Y. Lam, B. E. Steinberg, M. C. Dinauer, M. A. O. Magalhaes, M. Glogauer, S. Grinstein, and J. H. Brumell Activation of antibacterial autophagy by NADPH oxidases PNAS, April 14, 2009; 106(15): 6226 - 6231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Cornish, B. J. Hurtgen, K. McInnerney, N. L. Burritt, R. M. Taylor, J. N. Jarvis, S. Y. Wang, and J. B. Burritt Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Independent Resistance to Aspergillus fumigatus in Alveolar Macrophages J. Immunol., May 15, 2008; 180(10): 6854 - 6867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yao, I. Edirisinghe, S.-R. Yang, S. Rajendrasozhan, A. Kode, S. Caito, D. Adenuga, and I. Rahman Genetic Ablation of NADPH Oxidase Enhances Susceptibility to Cigarette Smoke-Induced Lung Inflammation and Emphysema in Mice Am. J. Pathol., May 1, 2008; 172(5): 1222 - 1237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kanayama and Y. Miyamoto Apoptosis triggered by phagocytosis-related oxidative stress through FLIPS down-regulation and JNK activation J. Leukoc. Biol., November 1, 2007; 82(5): 1344 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Holland, F. R. DeLeo, H. Z. Elloumi, A. P. Hsu, G. Uzel, N. Brodsky, A. F. Freeman, A. Demidowich, J. Davis, M. L. Turner, et al. STAT3 Mutations in the Hyper-IgE Syndrome N. Engl. J. Med., October 18, 2007; 357(16): 1608 - 1619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Wilkie, M. C. M. Vissers, M. Dragunow, and M. B. Hampton A Functional NADPH Oxidase Prevents Caspase Involvement in the Clearance of Phagocytic Neutrophils Infect. Immun., July 1, 2007; 75(7): 3256 - 3263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Blomgran, L. Zheng, and O. Stendahl Cathepsin-cleaved Bid promotes apoptosis in human neutrophils via oxidative stress-induced lysosomal membrane permeabilization J. Leukoc. Biol., May 1, 2007; 81(5): 1213 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bedard and K.-H. Krause The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology Physiol Rev, January 1, 2007; 87(1): 245 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Kobayashi, J. M. Voyich, A. R. Whitney, and F. R. DeLeo Spontaneous neutrophil apoptosis and regulation of cell survival by granulocyte macrophage-colony stimulating factor J. Leukoc. Biol., December 1, 2005; 78(6): 1408 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Musser and F. R. DeLeo Toward a Genome-Wide Systems Biology Analysis of Host-Pathogen Interactions in Group A Streptococcus Am. J. Pathol., December 1, 2005; 167(6): 1461 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sethy-Coraci, L. W. Crock, and S. C. Silverstein PAF-receptor antagonists, lovastatin, and the PTK inhibitor genistein inhibit H2O2 secretion by macrophages cultured on oxidized-LDL matrices J. Leukoc. Biol., November 1, 2005; 78(5): 1166 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Voyich, K. R. Braughton, D. E. Sturdevant, A. R. Whitney, B. Said-Salim, S. F. Porcella, R. D. Long, D. W. Dorward, D. J. Gardner, B. N. Kreiswirth, et al. Insights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils J. Immunol., September 15, 2005; 175(6): 3907 - 3919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Condliffe, K. Davidson, K. E. Anderson, C. D. Ellson, T. Crabbe, K. Okkenhaug, B. Vanhaesebroeck, M. Turner, L. Webb, M. P. Wymann, et al. Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils Blood, August 15, 2005; 106(4): 1432 - 1440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Borjesson, S. D. Kobayashi, A. R. Whitney, J. M. Voyich, C. M. Argue, and F. R. DeLeo Insights into Pathogen Immune Evasion Mechanisms: Anaplasma phagocytophilum Fails to Induce an Apoptosis Differentiation Program in Human Neutrophils J. Immunol., May 15, 2005; 174(10): 6364 - 6372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bylund, P. A. Campsall, R. C. Ma, B.-A. D. Conway, and D. P. Speert Burkholderia cenocepacia Induces Neutrophil Necrosis in Chronic Granulomatous Disease J. Immunol., March 15, 2005; 174(6): 3562 - 3569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Dinauer Chronic Granulomatous Disease and Other Disorders of Phagocyte Function Hematology, January 1, 2005; 2005(1): 89 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bellocchio, S. Moretti, K. Perruccio, F. Fallarino, S. Bozza, C. Montagnoli, P. Mosci, G. B. Lipford, L. Pitzurra, and L. Romani TLRs Govern Neutrophil Activity in Aspergillosis J. Immunol., December 15, 2004; 173(12): 7406 - 7415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Rothfork, G. S. Timmins, M. N. Harris, X. Chen, A. J. Lusis, M. Otto, A. L. Cheung, and H. D. Gresham Inactivation of a bacterial virulence pheromone by phagocyte-derived oxidants: New role for the NADPH oxidase in host defense PNAS, September 21, 2004; 101(38): 13867 - 13872. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
