| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Ludwig Institute for Cancer Research, Brussels Branch, Brussels, Belgium;
Cellular Genetics Unit, Institute of Cellular Pathology, Université de Louvain, Brussels, Belgium; and
Department of Dermatology, University Hospital of Erlangen, Erlangen, Germany
Vaccination with mature, monocyte-derived dendritic cells (DC) pulsed with the MAGE-3168–176 peptide, which is presented by HLA-A1, has been reported to induce tumor regressions and CTL in some advanced stage IV melanoma patients. We present here a precise evaluation of the level of some of these anti-MAGE-3.A1 CTL responses and an analysis of their clonal diversity. Blood lymphocytes were stimulated with the MAGE-3.A1 peptide under limiting dilution conditions and assayed with an A1/MAGE-3 tetramer. This was followed by the cloning of the tetramer-positive cells and by TCR sequence analysis of the CTL clones that lysed targets expressing MAGE-3.A1. We also used direct ex vivo tetramer staining of CD8 cells, sorting, and cloning of the positive cells. In three patients who showed regression of some of their metastases after vaccination, CTL responses were observed with frequencies ranging from 7 x 10-6 to 9 x 10-4 of CD8+ blood T lymphocytes, representing an increase of 20- to 400-fold of the frequencies found before immunization. A fourth patient showed neither tumor regression nor an anti-MAGE-3.A1 CTL response. In each of the responses, several CTL clones were amplified. This polyclonality contrasts with the monoclonality of the CTL responses observed in patients vaccinated with MAGE-3.A1 peptide or with an ALVAC recombinant virus coding for this antigenic peptide.
This article has been cited by other articles:
|
|
 |
|
  T. Connerotte, A. Van Pel, D. Godelaine, E. Tartour, B. Schuler-Thurner, S. Lucas, K. Thielemans, G. Schuler, and P. G. Coulie Functions of Anti-MAGE T-Cells Induced in Melanoma Patients under Different Vaccination Modalities Cancer Res., May 15, 2008; 68(10): 3931 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Carrasco, A. Van Pel, B. Neyns, B. Lethe, F. Brasseur, N. Renkvist, P. van der Bruggen, N. van Baren, R. Paulus, K. Thielemans, et al. Vaccination of a Melanoma Patient with Mature Dendritic Cells Pulsed with MAGE-3 Peptides Triggers the Activity of Nonvaccine Anti-Tumor Cells J. Immunol., March 1, 2008; 180(5): 3585 - 3593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Michalek, I. Kocak, V. Fait, J. Zaloudik, and R. Hajek Detection and Long-Term In Vivo Monitoring of Individual Tumor-Specific T Cell Clones in Patients with Metastatic Melanoma J. Immunol., June 1, 2007; 178(11): 6789 - 6795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Carrasco, D. Godelaine, A. Van Pel, T. Boon, and P. van der Bruggen CD45RA on human CD8 T cells is sensitive to the time elapsed since the last antigenic stimulation Blood, November 1, 2006; 108(9): 2897 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fukuyama, T. Hanagiri, M. Takenoyama, Y. Ichiki, M. Mizukami, T. So, M. Sugaya, T. So, K. Sugio, and K. Yasumoto Identification of a New Cancer/Germline Gene, KK-LC-1, Encoding an Antigen Recognized by Autologous CTL Induced on Human Lung Adenocarcinoma. Cancer Res., May 1, 2006; 66(9): 4922 - 4928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. van Baren, M.-C. Bonnet, B. Dreno, A. Khammari, T. Dorval, S. Piperno-Neumann, D. Lienard, D. Speiser, M. Marchand, V. G. Brichard, et al. Tumoral and Immunologic Response After Vaccination of Melanoma Patients With an ALVAC Virus Encoding MAGE Antigens Recognized by T Cells J. Clin. Oncol., December 10, 2005; 23(35): 9008 - 9021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Linette, D. Zhang, F. S. Hodi, E. P. Jonasch, S. Longerich, C. P. Stowell, I. J. Webb, H. Daley, R. J. Soiffer, A. M. Cheung, et al. Immunization Using Autologous Dendritic Cells Pulsed with the Melanoma-Associated Antigen gp100-Derived G280-9V Peptide Elicits CD8+ Immunity Clin. Cancer Res., November 1, 2005; 11(21): 7692 - 7699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nagata, T. Hanagiri, M. Takenoyama, T. Fukuyama, M. Mizukami, T. So, Y. Ichiki, M. Sugaya, K. Sugio, and K. Yasumoto Identification of the HLA-Cw*0702-Restricted Tumor-Associated Antigen Recognized by a CTL Clone from a Lung Cancer Patient Clin. Cancer Res., July 15, 2005; 11(14): 5265 - 5272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Schaft, J. Dorrie, P. Thumann, V. E. Beck, I. Muller, E. S. Schultz, E. Kampgen, D. Dieckmann, and G. Schuler Generation of an Optimized Polyvalent Monocyte-Derived Dendritic Cell Vaccine by Transfecting Defined RNAs after Rather Than before Maturation J. Immunol., March 1, 2005; 174(5): 3087 - 3097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, Z. Sun, H. Nicolay, R. G. Meyer, N. Renkvist, V. Stroobant, J. Corthals, J. Carrasco, A. M. M. Eggermont, M. Marchand, et al. Monitoring of Anti-Vaccine CD4 T Cell Frequencies in Melanoma Patients Vaccinated with a MAGE-3 Protein J. Immunol., February 15, 2005; 174(4): 2404 - 2411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lonchay, P. van der Bruggen, T. Connerotte, T. Hanagiri, P. Coulie, D. Colau, S. Lucas, A. Van Pel, K. Thielemans, N. van Baren, et al. Correlation between tumor regression and T cell responses in melanoma patients vaccinated with a MAGE antigen PNAS, October 5, 2004; 101(suppl_2): 14631 - 14638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Paczesny, J. Banchereau, K. M. Wittkowski, G. Saracino, J. Fay, and A. K. Palucka Expansion of Melanoma-specific Cytolytic CD8+ T Cell Precursors in Patients with Metastatic Melanoma Vaccinated with CD34+ Progenitor-derived Dendritic Cells J. Exp. Med., June 7, 2004; 199(11): 1503 - 1511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Karanikas, C. Lurquin, D. Colau, N. van Baren, C. De Smet, B. Lethe, T. Connerotte, V. Corbiere, M.-A. Demoitie, D. Lienard, et al. Monoclonal Anti-MAGE-3 CTL Responses in Melanoma Patients Displaying Tumor Regression after Vaccination with a Recombinant Canarypox Virus J. Immunol., November 1, 2003; 171(9): 4898 - 4904. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
