| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Herman B. Wells Center for Pediatric Research, Departments of Pediatrics (Hematology/Oncology), Microbiology/Immunology, and Medical and Molecular Genetics, James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis, IN 46202;
Inha University College of Medicine, Incheon, Korea; and
Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria
Vav1 is a hemopoietic-specific Rho/Rac guanine nucleotide exchange factor that plays a prominent role in responses to multisubunit immune recognition receptors in lymphoid cells, but its contribution to regulation of neutrophil functions is unknown. Activated Rho family GTPases are critical participants in neutrophil signaling cascades initiated by binding of FMLP and other chemoattractants to their cognate G protein-coupled receptors. Therefore, we investigated whether Vav1 regulates chemoattractant-induced responses in neutrophils. We found that superoxide production elicited by FMLP in Vav1-/- murine neutrophils isolated from either bone marrow or from peritoneal exudates was substantially reduced compared with that of wild type. Filamentous actin generation in FMLP-stimulated Vav1-/- neutrophils was also markedly reduced, whereas it was normal in response to IL-8 or leukotriene B4. FMLP induced tyrosine phosphorylation of Vav1, whereas IL-8 or leukotriene B4 did not, correlating with the requirement for Vav1 in chemoattractant-stimulated filamentous actin generation. Neutrophil motility in vitro and neutrophil mobilization into peripheral blood in vivo elicited by FMLP were both decreased in Vav1-/- mice. Hence, this study defines a new role for Vav1 in regulating granulocytic leukocytes as well as linking Vav1 to specific cellular responses downstream of a seven transmembrane domain receptor.
This article has been cited by other articles:
|
|
 |
|
  M. Phillipson, B. Heit, S. A. Parsons, B. Petri, S. C. Mullaly, P. Colarusso, R. M. Gower, G. Neely, S. I. Simon, and P. Kubes Vav1 Is Essential for Mechanotactic Crawling and Migration of Neutrophils out of the Inflamed Microvasculature J. Immunol., June 1, 2009; 182(11): 6870 - 6878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Ye, F. Boulay, J. M. Wang, C. Dahlgren, C. Gerard, M. Parmentier, C. N. Serhan, and P. M. Murphy International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the Formyl Peptide Receptor (FPR) Family Pharmacol. Rev., June 1, 2009; 61(2): 119 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mitchell, A. Lo, M. R. Logan, P. Lacy, and G. Eitzen Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1354 - C1365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Roepstorff, I. Rasmussen, M. Sawada, C. Cudre-Maroux, P. Salmon, G. Bokoch, B. van Deurs, and F. Vilhardt Stimulus-dependent Regulation of the Phagocyte NADPH Oxidase by a VAV1, Rac1, and PAK1 Signaling Axis J. Biol. Chem., March 21, 2008; 283(12): 7983 - 7993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zarbock and K. Ley Mechanisms and Consequences of Neutrophil Interaction with the Endothelium Am. J. Pathol., January 1, 2008; 172(1): 1 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Miletic, D. B. Graham, V. Montgrain, K. Fujikawa, T. Kloeppel, K. Brim, B. Weaver, R. Schreiber, R. Xavier, and W. Swat Vav proteins control MyD88-dependent oxidative burst Blood, April 15, 2007; 109(8): 3360 - 3368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Kuijpers, R. v. Bruggen, N. Kamerbeek, A. T. J. Tool, G. Hicsonmez, A. Gurgey, A. Karow, A. J. Verhoeven, K. Seeger, O. Sanal, et al. Natural history and early diagnosis of LAD-1/variant syndrome Blood, April 15, 2007; 109(8): 3529 - 3537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zhao, P. Nalbant, M. Hoshino, X. Dong, D. Wu, and G. M. Bokoch Signaling requirements for translocation of P-Rex1, a key Rac2 exchange factor involved in chemoattractant-stimulated human neutrophil function J. Leukoc. Biol., April 1, 2007; 81(4): 1127 - 1136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fumagalli, H. Zhang, A. Baruzzi, C. A. Lowell, and G. Berton The Src Family Kinases Hck and Fgr Regulate Neutrophil Responses to N-Formyl-Methionyl-Leucyl-Phenylalanine J. Immunol., March 15, 2007; 178(6): 3874 - 3885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ming, S. Li, D. D. Billadeau, L. A. Quilliam, and M. C. Dinauer The Rac Effector p67phox Regulates Phagocyte NADPH Oxidase by Stimulating Vav1 Guanine Nucleotide Exchange Activity Mol. Cell. Biol., January 1, 2007; 27(1): 312 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Utomo, X. Cullere, M. Glogauer, W. Swat, and T. N. Mayadas Vav Proteins in Neutrophils Are Required for Fc{gamma}R-Mediated Signaling to Rac GTPases and Nicotinamide Adenine Dinucleotide Phosphate Oxidase Component p40(phox) J. Immunol., November 1, 2006; 177(9): 6388 - 6397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Ellson, K. Davidson, G. J. Ferguson, R. O'Connor, L. R. Stephens, and P. T. Hawkins Neutrophils from p40phox-/- mice exhibit severe defects in NADPH oxidase regulation and oxidant-dependent bacterial killing J. Exp. Med., August 7, 2006; 203(8): 1927 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Giagulli, L. Ottoboni, E. Caveggion, B. Rossi, C. Lowell, G. Constantin, C. Laudanna, and G. Berton The Src Family Kinases Hck and Fgr Are Dispensable for Inside-Out, Chemoattractant-Induced Signaling Regulating beta2 Integrin Affinity and Valency in Neutrophils, but Are Required for beta2 Integrin-Mediated Outside-In Signaling Involved in Sustained Adhesion J. Immunol., July 1, 2006; 177(1): 604 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kim and M. C. Dinauer Impaired NADPH oxidase activity in Rac2-deficient murine neutrophils does not result from defective translocation of p47phox and p67phox and can be rescued by exogenous arachidonic acid J. Leukoc. Biol., January 1, 2006; 79(1): 223 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yamauchi, C. C. Marchal, J. Molitoris, N. Pech, U. Knaus, J. Towe, S. J. Atkinson, and M. C. Dinauer Rac GTPase Isoform-specific Regulation of NADPH Oxidase and Chemotaxis in Murine Neutrophils in Vivo: ROLE OF THE C-TERMINAL POLYBASIC DOMAIN J. Biol. Chem., January 14, 2005; 280(2): 953 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Brandes and J. Kreuzer Vascular NADPH oxidases: molecular mechanisms of activation Cardiovasc Res, January 1, 2005; 65(1): 16 - 27. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
