| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Medicine, Division of Oncology, Stanford University Medical Center;
Institut André Lwoff, Institut de la Santé et de la Recherche Médicale Unité 268, Hôpital Paul Brousse, Villejuif, France; and
Department of Immunology, Duke University Medical Center, Durham, NC
CD81 is a widely expressed tetraspanin that associates in B cells with CD19 in the CD19-CD21-CD81 signaling complex. CD81 is necessary for normal CD19 expression; cd81-/- B cells express lower levels of CD19, especially cd81-/- small pre-BII cells, which are almost devoid of surface CD19. The dependence of CD19 expression on CD81 is specific to this particular tetraspanin since cd9-/- B cells express normal levels of CD19. Furthermore, expression of human CD81 in mouse cd81-/- B cells restored surface CD19 to normal levels. Quantitative analysis of CD19 mRNA demonstrated normal levels, even in cd81-/- pre-BII cells. Analysis of CD19 at the protein level identified two CD19 glycoforms in both wild-type and cd81-/- B cells. The higher Mr glycoform is significantly reduced in cd81-/- B cells and is endoglycosidase H (endo-H) resistant. In contrast, the low Mr glycoform is comparably expressed in cd81-/- and in wild-type B cells and is endo-H sensitive. Because endo-H sensitivity is tightly correlated with endoplasmic reticulum localization, we suggest that the dependency of CD19 expression on CD81 occurs in a postendoplasmic reticulum compartment where CD81 is necessary for normal trafficking or for surface membrane stability of CD19.
This article has been cited by other articles:
|
|
 |
|
  C. Arduise, T. Abache, L. Li, M. Billard, A. Chabanon, A. Ludwig, P. Mauduit, C. Boucheix, E. Rubinstein, and F. Le Naour Tetraspanins Regulate ADAM10-Mediated Cleavage of TNF-{alpha} and Epidermal Growth Factor J. Immunol., November 15, 2008; 181(10): 7002 - 7013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lekishvili, E. Fromm, M. Mujoomdar, and F. Berditchevski The tumour-associated antigen L6 (L6-Ag) is recruited to the tetraspanin-enriched microdomains: implication for tumour cell motility J. Cell Sci., March 1, 2008; 121(5): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Soulas-Sprauel, G. Le Guyader, P. Rivera-Munoz, V. Abramowski, C. Olivier-Martin, C. Goujet-Zalc, P. Charneau, and J.-P. de Villartay Role for DNA repair factor XRCC4 in immunoglobulin class switch recombination J. Exp. Med., July 9, 2007; 204(7): 1717 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Latysheva, G. Muratov, S. Rajesh, M. Padgett, N. A. Hotchin, M. Overduin, and F. Berditchevski Syntenin-1 Is a New Component of Tetraspanin-Enriched Microdomains: Mechanisms and Consequences of the Interaction of Syntenin-1 with CD63 Mol. Cell. Biol., October 15, 2006; 26(20): 7707 - 7718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hobeika, S. Thiemann, B. Storch, H. Jumaa, P. J. Nielsen, R. Pelanda, and M. Reth Testing gene function early in the B cell lineage in mb1-cre mice PNAS, September 12, 2006; 103(37): 13789 - 13794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Silvie, S. Charrin, M. Billard, J.-F. Franetich, K. L. Clark, G.-J. van Gemert, R. W. Sauerwein, F. Dautry, C. Boucheix, D. Mazier, et al. Cholesterol contributes to the organization of tetraspanin-enriched microdomains and to CD81-dependent infection by malaria sporozoites J. Cell Sci., May 15, 2006; 119(10): 1992 - 2002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. van Zelm, I. Reisli, M. van der Burg, D. Castano, C. J.M. van Noesel, M. J.D. van Tol, C. Woellner, B. Grimbacher, P. J. Patino, J. J.M. van Dongen, et al. An antibody-deficiency syndrome due to mutations in the CD19 gene. N. Engl. J. Med., May 4, 2006; 354(18): 1901 - 1912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shoham, R. Rajapaksa, C.-C. Kuo, J. Haimovich, and S. Levy Building of the Tetraspanin Web: Distinct Structural Domains of CD81 Function in Different Cellular Compartments Mol. Cell. Biol., February 15, 2006; 26(4): 1373 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Stein, J. C. Go, P. Primakoff, and D. G. Myles Defects in Secretory Pathway Trafficking During Sperm Development in Adam2 Knockout Mice Biol Reprod, November 1, 2005; 73(5): 1032 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cariappa, T. Shoham, H. Liu, S. Levy, C. Boucheix, and S. Pillai The CD9 Tetraspanin Is Not Required for the Development of Peripheral B Cells or for Humoral Immunity J. Immunol., September 1, 2005; 175(5): 2925 - 2930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. A. Hu, F.-X. Liang, G. Zhou, L. Tu, C.-H. A. Tang, J. Zhou, G. Kreibich, and T.-T. Sun Assembly of Urothelial Plaques: Tetraspanin Function in Membrane Protein Trafficking Mol. Biol. Cell, September 1, 2005; 16(9): 3937 - 3950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Levy and T. Shoham Protein-Protein Interactions in the Tetraspanin Web Physiology, August 1, 2005; 20(4): 218 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Barreiro, M. Yanez-Mo, M. Sala-Valdes, M. D. Gutierrez-Lopez, S. Ovalle, A. Higginbottom, P. N. Monk, C. Cabanas, and F. Sanchez-Madrid Endothelial tetraspanin microdomains regulate leukocyte firm adhesion during extravasation Blood, April 1, 2005; 105(7): 2852 - 2861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cherukuri, T. Shoham, H. W. Sohn, S. Levy, S. Brooks, R. Carter, and S. K. Pierce The Tetraspanin CD81 Is Necessary for Partitioning of Coligated CD19/CD21-B Cell Antigen Receptor Complexes into Signaling-Active Lipid Rafts J. Immunol., January 1, 2004; 172(1): 370 - 380. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
