| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Laboratory of Vaccine Research and
Division of Information Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010; and
Division of Medicine, Johns Hopkins University Medical School, Baltimore, MD 21218
We evaluated immunogenicity of a novel Th-CTL fusion peptide composed of the pan DR Th epitope and a CTL epitope derived from HIV-pol in two transgenic HLA-A*0201/Kb mouse models. The immunogenicity of peptides of this structure is highly dependent on coadministered cytosine-phosphate-guanine DNA. Initial evaluations of peptide-specific immunity are based on results of chromium release assay, intracellular cytokine, and tetramer staining. Significant cytotoxic T cell responses are found upon a single immunization with as low as 0.1 nmol both peptide and cytosine-phosphate-guanine DNA. Splenocytes from immunized mice recognize naturally processed HIV-pol expressed from vaccinia virus (pol-VV). Translation of immunologic criteria into more relevant assays was pursued using systemic challenge of immunized mice with pol-VV. Only mice receiving both peptide and DNA together successfully cleared upward of 6 logs of virus from ovaries, compared with controls. Challenge with pol-VV by intranasal route of intranasal immunized mice showed a significant reduction in the levels of VV in lung compared with naive mice. A convincing demonstration of the relevance of these vaccines is the robust lysis of HIV-infected Jurkat T cells (JA2/R7/Hyg) by immune splenocytes from peptide- and DNA-immunized mice. This surprisingly effective immunization merits consideration for clinical evaluation, because it succeeded in causing immune recognition and lysis of cells infected with its target virus and reduction in titer of highly pathogenic VV.
Related articles in The JI:
This article has been cited by other articles:
|
|
 |
|
  J. Botten, J. L. Whitton, P. Barrowman, J. Sidney, J. K. Whitmire, J. Alexander, J. P. C. Ting, H.-H. Bui, A. Sette, and M. J. Buchmeier HLA-A2-Restricted Protection against Lethal Lymphocytic Choriomeningitis J. Virol., March 1, 2007; 81(5): 2307 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cornberg, B. S. Sheridan, F. M. Saccoccio, M. A. Brehm, and L. K. Selin Protection against Vaccinia Virus Challenge by CD8 Memory T Cells Resolved by Molecular Mimicry J. Virol., January 15, 2007; 81(2): 934 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Botten, J. Alexander, V. Pasquetto, J. Sidney, P. Barrowman, J. Ting, B. Peters, S. Southwood, B. Stewart, M. P. Rodriguez-Carreno, et al. Identification of Protective Lassa Virus Epitopes That Are Restricted by HLA-A2. J. Virol., September 1, 2006; 80(17): 8351 - 8361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krymskaya, M. C. Sharma, J. Martinez, W. Haq, E. C. Huang, A. P. Limaye, D. J. Diamond, and S. F. Lacey Cross-Reactivity of T Lymphocytes Recognizing a Human Cytotoxic T-Lymphocyte Epitope within BK and JC Virus VP1 Polypeptides J. Virol., September 1, 2005; 79(17): 11170 - 11178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shams, P. Klucar, S. E. Weis, A. Lalvani, P. K. Moonan, H. Safi, B. Wizel, K. Ewer, G. T. Nepom, D. M. Lewinsohn, et al. Characterization of a Mycobacterium tuberculosis Peptide That Is Recognized by Human CD4+ and CD8+ T Cells in the Context of Multiple HLA Alleles J. Immunol., August 1, 2004; 173(3): 1966 - 1977. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
