| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China
In this study, we demonstrate that Dermatophagoides farinae (Der f), a major source of airborne allergens, but not OVA, could rapidly activate mast cells in mice. This was indicated by an elevation of serum mouse mast cell protease 1, a mast cell-specific proteinase, as early as 30 min after intratracheal challenge. Administration of sodium cromoglycate (40 mg/kg, i.p., 1 h before Der f instillation), a mast cell stabilizer, not only suppressed acute mouse mast cell protease 1 production but also attenuated the allergic airway inflammation provoked by repetitive Der f challenge in mice (five times at 1-wk interval). Der f induced the expression of mRNA for TNF-
, IL-1
, IL-4, IL-6, IL-9, and IL-13 in mastocytoma P815 cells and stimulated both P815 cells and bone marrow-derived mast cells to produce IL-4, IL-6, and TNF- in a dose- and time-dependent manner. Cycloheximide as well as sodium cromoglycate blocked the Der f-induced IL-4 production, indicating a de novo protein synthesis process. Supernatants of Der f-stimulated mast cells chemoattracted monocytes and T lymphocytes; they up-regulated the expression of costimulatory B7 molecules, eotaxin, RANTES, monocyte chemoattractant protein 1, and IFN-inducible protein 10 mRNA of alveolar macrophages; they supported PHA-induced T cell proliferation; and they promoted Th2 cell development. Our data indicate that mast cells may be an important cell type during the initiation of Der f sensitization in the airway by modulating the function of alveolar macrophages and T cells.
This article has been cited by other articles:
|
|
 |
|
  N. A. Barrett, A. Maekawa, O. M. Rahman, K. F. Austen, and Y. Kanaoka Dectin-2 Recognition of House Dust Mite Triggers Cysteinyl Leukotriene Generation by Dendritic Cells J. Immunol., January 15, 2009; 182(2): 1119 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kulka, N. Fukuishi, M. Rottem, Y. A. Mekori, and D. D. Metcalfe Mast cells, which interact with Escherichia coli, up-regulate genes associated with innate immunity and become less responsive to Fc{epsilon}RI-mediated activation J. Leukoc. Biol., February 1, 2006; 79(2): 339 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-T. Chen and J. T. Kung CD1d-Independent Developmental Acquisition of Prompt IL-4 Gene Inducibility in Thymus CD161(NK1)-CD44lowCD4+CD8- T Cells Is Associated with Complementarity Determining Region 3-Diverse and Biased V{beta}2/V{beta}7/V{beta}8/V{alpha}3.2 T Cell Receptor Usage J. Immunol., November 15, 2005; 175(10): 6537 - 6550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Orinska, E. Bulanova, V. Budagian, M. Metz, M. Maurer, and S. Bulfone-Paus TLR3-induced activation of mast cells modulates CD8+ T-cell recruitment Blood, August 1, 2005; 106(3): 978 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Boyce and K. F. Austen No audible wheezing: nuggets and conundrums from mouse asthma models J. Exp. Med., June 20, 2005; 201(12): 1869 - 1873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nemmar, P. H.M. Hoet, J. Vermylen, B. Nemery, and M. F. Hoylaerts Pharmacological Stabilization of Mast Cells Abrogates Late Thrombotic Events Induced by Diesel Exhaust Particles in Hamsters Circulation, September 21, 2004; 110(12): 1670 - 1677. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
