| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Turku Centre for Biotechnology,
Drug Discovery Graduate School,
Turku Graduate School of Biomedical Sciences, and
Turku Centre for Computer Science, University of Turku and Åbo Akademi University, Turku, Finland
IL-4, primarily produced by T cells, mast cells, and basophiles, is a cytokine which has pleiotropic effects on the immune system. IL-4 induces T cells to differentiate to Th2 cells and activated B lymphocytes to proliferate and to synthesize IgE and IgG1. IL-4 is particularly important for the development and perpetuation of asthma and allergy. Stat6 is the protein activated by signal transduction through the IL-4R, and studies with knockout mice demonstrate that Stat6 is critical for a number of IL-4-mediated functions including Th2 development and production of IgE. In the present study, novel IL-4- and Stat6-regulated genes were discovered by using Stat6-/- mice and Affymetrix oligonucleotide arrays. Genes regulated by IL-4 were identified by comparing the gene expression profile of the wild-type T cells induced to polarize to the Th2 direction (CD3/CD28 activation + IL-4) to gene expression profile of the cells induced to proliferate (CD3/CD28 activation alone). Stat6-regulated genes were identified by comparing the cells isolated from the wild-type and Stat6-/- mice; in this experiment the cells were induced to differentiate to the Th2 direction (CD3/CD28 activation + IL-4). Our study demonstrates that a number a novel genes are regulated by IL-4 through Stat6-dependent and -independent pathways. Moreover, elucidation of kinetics of gene expression at early stages of cell differentiation reveals several genes regulated rapidly during the process, suggesting their importance for the differentiation process.
This article has been cited by other articles:
|
|
 |
|
  R. J. Ingram, J. D. Isaacs, G. Kaur, D. E. Lowther, C. J. Reynolds, R. J. Boyton, J. Collinge, G. S. Jackson, and D. M. Altmann A role of cellular prion protein in programming T-cell cytokine responses in disease FASEB J, June 1, 2009; 23(6): 1672 - 1684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-J. Filen, S. Filen, R. Moulder, S. Tuomela, H. Ahlfors, A. West, P. Kouvonen, S. Kantola, M. Bjorkman, M. Katajamaa, et al. Quantitative Proteomics Reveals GIMAP Family Proteins 1 and 4 to Be Differentially Regulated during Human T Helper Cell Differentiation Mol. Cell. Proteomics, January 1, 2009; 8(1): 32 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Atreya, C. C Schimanski, C. Becker, S. Wirtz, H. Dornhoff, E. Schnurer, M. R Berger, P. R Galle, W. Herr, and M. F Neurath The T-box transcription factor eomesodermin controls CD8 T cell activity and lymph node metastasis in human colorectal cancer Gut, November 1, 2007; 56(11): 1572 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Elo, H. Jarvenpaa, M. Oresic, R. Lahesmaa, and T. Aittokallio Systematic construction of gene coexpression networks with applications to human T helper cell differentiation process Bioinformatics, August 15, 2007; 23(16): 2096 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Lund, M. Loytomaki, T. Naumanen, C. Dixon, Z. Chen, H. Ahlfors, S. Tuomela, J. Tahvanainen, J. Scheinin, T. Henttinen, et al. Genome-Wide Identification of Novel Genes Involved in Early Th1 and Th2 Cell Differentiation J. Immunol., March 15, 2007; 178(6): 3648 - 3660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Murray The JAK-STAT Signaling Pathway: Input and Output Integration J. Immunol., March 1, 2007; 178(5): 2623 - 2629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Rautajoki, E. M. Marttila, T. A. Nyman, and R. Lahesmaa Interleukin-4 Inhibits Caspase-3 by Regulating Several Proteins in the Fas Pathway during Initial Stages of Human T Helper 2 Cell Differentiation Mol. Cell. Proteomics, February 1, 2007; 6(2): 238 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suto, A. L. Wurster, S. L. Reiner, and M. J. Grusby IL-21 Inhibits IFN-{gamma} Production in Developing Th1 Cells through the Repression of Eomesodermin Expression J. Immunol., September 15, 2006; 177(6): 3721 - 3727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bosco, K. L. McKenna, C. J. Devitt, M. J. Firth, P. D. Sly, and P. G. Holt Identification of novel th2-associated genes in T memory responses to allergens. J. Immunol., April 15, 2006; 176(8): 4766 - 4777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goenka and M. Boothby Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor. PNAS, March 14, 2006; 103(11): 4210 - 4215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhu, R. Hart, M. S. Chang, J.-W. Kim, S. Y. Lee, Y. A. Cao, D. Mock, E. Ke, B. Saunders, A. Alexander, et al. Analysis of the Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands J. Immunol., December 15, 2004; 173(12): 7141 - 7149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jeyaseelan, H. W. Chu, S. K. Young, and G. S. Worthen Transcriptional Profiling of Lipopolysaccharide-Induced Acute Lung Injury Infect. Immun., December 1, 2004; 72(12): 7247 - 7256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Puig-Kroger, D. Serrano-Gomez, E. Caparros, A. Dominguez-Soto, M. Relloso, M. Colmenares, L. Martinez-Munoz, N. Longo, N. Sanchez-Sanchez, M. Rincon, et al. Regulated Expression of the Pathogen Receptor Dendritic Cell-specific Intercellular Adhesion Molecule 3 (ICAM-3)-grabbing Nonintegrin in THP-1 Human Leukemic Cells, Monocytes, and Macrophages J. Biol. Chem., June 11, 2004; 279(24): 25680 - 25688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Lund, Z. Chen, J. Scheinin, and R. Lahesmaa Early Target Genes of IL-12 and STAT4 Signaling in Th Cells J. Immunol., June 1, 2004; 172(11): 6775 - 6782. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
