HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sedegah, M. Articles by Hoffman, S. L. Search for Related Content PubMed PubMed Citation Articles by Sedegah, M. Articles by Hoffman, S. L.

Successful Induction of CD8 T Cell-Dependent Protection Against Malaria by Sequential Immunization with DNA and Recombinant Poxvirus of Neonatal Mice Born to Immune Mothers ¹

Martha Sedegah^*, Maria Belmonte^*, Judith E. Epstein^*, Claire-Anne Siegrist, Walter R. Weiss^*, Trevor R. Jones^*, Minh Lu^*, Daniel J. Carucci^* and Stephen L. Hoffman^2,*

^* Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910; and World Health Organization Collaborating Centre for Neonatal Vaccinology, Department of Pathology and Pediatrics, University of Geneva, Geneva, Switzerland

In some parts of Africa, 50% of deaths attributed to malaria occur in infants less than 8 mo. Thus, immunization against malaria may have to begin in the neonatal period, when neonates have maternally acquired Abs against malaria parasite proteins. Many malaria vaccines in development rely upon CD8 cells as immune effectors. Some studies indicate that neonates do not mount optimal CD8 cell responses. We report that BALB/c mice first immunized as neonates (7 days) with a Plasmodium yoelii circumsporozoite protein (PyCSP) DNA vaccine mixed with a plasmid expressing murine GM-CSF (DG) and boosted at 28 days with poxvirus expressing PyCSP were protected (93%) as well as mice immunized entirely as adults (70%). Protection was dependent on CD8 cells, and mice had excellent anti-PyCSP IFN- and cytotoxic T lymphocyte responses. Mice born of mothers previously exposed to P. yoelii parasites or immunized with the vaccine were protected and had excellent T cell responses. These data support assessment of this immunization strategy in neonates/young infants in areas in which malaria exacts its greatest toll.

This article has been cited by other articles:

				J. J. Donnelly, B. Wahren, and M. A. Liu DNA Vaccines: Progress and Challenges J. Immunol., July 15, 2005; 175(2): 633 - 639. [Abstract] [Full Text] [PDF]

				A. V. E. Capozzo, L. Cuberos, M. M. Levine, and M. F. Pasetti Mucosally Delivered Salmonella Live Vector Vaccines Elicit Potent Immune Responses against a Foreign Antigen in Neonatal Mice Born to Naive and Immune Mothers Infect. Immun., August 1, 2004; 72(8): 4637 - 4646. [Abstract] [Full Text] [PDF]

				D. I. Stanisic, L. B. Martin, M. L. Gatton, and M. F. Good Inhibition of 19-kDa C-Terminal Region of Merozoite Surface Protein-1-Specific Antibody Responses in Neonatal Pups by Maternally Derived 19-kDa C-Terminal Region of Merozoite Surface Protein-1-Specific Antibodies but Not Whole Parasite-Specific Antibodies J. Immunol., May 1, 2004; 172(9): 5570 - 5581. [Abstract] [Full Text] [PDF]