The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Biddison, W. E.
Right arrow Articles by Reiter, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Biddison, W. E.
Right arrow Articles by Reiter, Y.
The Journal of Immunology, 2003, 171: 3064-3074.
Copyright © 2003 by The American Association of Immunologists

Tax and M1 Peptide/HLA-A2-Specific Fabs and T Cell Receptors Recognize Nonidentical Structural Features on Peptide/HLA-A2 Complexes 1

William E. Biddison2,*, Richard V. Turner*, Susan J. Gagnon*, Avital Lev{dagger}, Cyril J. Cohen{dagger} and Yoram Reiter{dagger}

* Molecular Immunology Section, Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892; and {dagger} Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel

Both TCRs and Ab molecules are capable of MHC-restricted recognition of peptide/MHC complexes. However, such MHC restriction is the predominant mode of recognition by T cells, but is extremely rare for B cells. The present study asks whether the dichotomy in Ag recognition modes of T and B cells could be due to fundamental differences in the methods by which TCRs and Abs recognize peptide/MHC complexes. We have compared MHC and peptide recognition by panels of CTL lines specific for the Tax and M1 peptides presented by HLA-A2 plus Tax and M1 peptide/HLA-A2-specific human Fabs that were selected from a naive phage display library. Collectively, the results indicate both striking similarities and important differences between Fab and TCR recognition of MHC and peptide components of the Tax and M1/HLA-A2 complexes. These findings suggest that these two classes of immunoreceptors have solved the problem of specific recognition of peptide/MHC complexes by nonidentical mechanisms. This conclusion is important in part because it indicates that Ab engineering approaches could produce second-generation Ab molecules that more closely mimic TCR fine specificity. Such efforts may produce more efficacious diagnostic and therapeutic agents.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. M. Truscott, X. Wang, L. Lybarger, W. E. Biddison, C. McBerry, J. M. Martinko, J. M. Connolly, G. P. Linette, D. H. Fremont, T. H. Hansen, et al.
Human Major Histocompatibility Complex (MHC) Class I Molecules with Disulfide Traps Secure Disease-related Antigenic Peptides and Exclude Competitor Peptides
J. Biol. Chem., March 21, 2008; 283(12): 7480 - 7490.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Zhu, H. J. Belmont, S. Price-Schiavi, B. Liu, H.-i. Lee, M. Fernandez, R. L. Wong, J. Builes, P. R. Rhode, and H. C. Wong
Visualization of p53264-272/HLA-A*0201 Complexes Naturally Presented on Tumor Cell Surface by a Multimeric Soluble Single-Chain T Cell Receptor.
J. Immunol., March 1, 2006; 176(5): 3223 - 3232.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
O. Y. Borbulevych, T. K. Baxter, Z. Yu, N. P. Restifo, and B. M. Baker
Increased Immunogenicity of an Anchor-Modified Tumor-Associated Antigen Is Due to the Enhanced Stability of the Peptide/MHC Complex: Implications for Vaccine Design
J. Immunol., April 15, 2005; 174(8): 4812 - 4820.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Kummer, A. Lev, Y. Reiter, and B. C. Biedermann
Vascular Endothelial Cells Have Impaired Capacity to Present Immunodominant, Antigenic Peptides: A Mechanism of Cell Type-Specific Immune Escape
J. Immunol., February 15, 2005; 174(4): 1947 - 1953.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hulsmeyer, P. Chames, R. C. Hillig, R. L. Stanfield, G. Held, P. G. Coulie, C. Alings, G. Wille, W. Saenger, B. Uchanska-Ziegler, et al.
A Major Histocompatibility Complex{middle dot}Peptide-restricted Antibody and T Cell Receptor Molecules Recognize Their Target by Distinct Binding Modes: CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ANTIGEN (HLA)-A1{middle dot}MAGE-A1 IN COMPLEX WITH FAB-HYB3
J. Biol. Chem., January 28, 2005; 280(4): 2972 - 2980.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Laugel, J. M. Boulter, N. Lissin, A. Vuidepot, Y. Li, E. Gostick, L. E. Crotty, D. C. Douek, J. Hemelaar, D. A. Price, et al.
Design of Soluble Recombinant T Cell Receptors for Antigen Targeting and T Cell Inhibition
J. Biol. Chem., January 21, 2005; 280(3): 1882 - 1892.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Mareeva, T. Lebedeva, N. Anikeeva, T. Manser, and Y. Sykulev
Antibody Specific for the Peptide{middle dot}Major Histocompatibility Complex: IS IT T CELL RECEPTOR-LIKE?
J. Biol. Chem., October 22, 2004; 279(43): 44243 - 44249.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.