The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grolleau, A.
Right arrow Articles by Mulé, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grolleau, A.
Right arrow Articles by Mulé, J. J.
The Journal of Immunology, 2003, 171: 2879-2888.
Copyright © 2003 by The American Association of Immunologists

Inducible Expression of Macrophage Receptor Marco by Dendritic Cells Following Phagocytic Uptake of Dead Cells Uncovered by Oligonucleotide Arrays 1

Annabelle Grolleau*, David E. Misek{dagger}, Rork Kuick{dagger}, Samir Hanash{dagger} and James J. Mulé2,*

Departments of * Surgery and {dagger} Pediatrics, University of Michigan Medical Center, Ann Arbor, MI 48109

The efficient Ag presenting and immunostimulatory capacity of dendritic cells (DCs) has led to the use of tumor Ag-pulsed DCs in treatment regimens for cancer. Although vaccine studies involving tumor lysate-pulsed DCs have been performed, little, if any, information is available on the effects of phagocytic uptake of tumor lysate on DC biology and function. We have investigated gene expression pattern differences between unpulsed DCs and tumor lysate-pulsed-DCs, using Affymetrix MG-U74Av2 oligonucleotide arrays, which contain ~12,000 genes and expressed sequence tags. Upon 24 h tumor lysate pulsing, the levels of 87 transcripts increased at least 3-fold while the levels of 121 transcripts were reduced by one-third or more, with accompanying p values <0.01. Most of these genes encoded proteins important for DC effector functions including cytokines, chemokines, and receptors, such as IL-12p40, macrophage inflammatory protein-2, and IL-6; Ag presentation, such as carboxypeptidase D and H2-DM; cell adhesion (e.g., EGF-like module containing, mucin-like, hormone receptor-like sequence 1, rhoB); and T cell activation. Interestingly, we observed a high level of expression of a novel member of the class A scavenger receptor family, macrophage receptor with collagenous structure (Marco). Marco is thought to play an important role in the immune response by mediating binding and phagocytosis, but also in the formation of lamellipodia-like structures and of dendritic processes. Therefore, we have identified a repertoire of genes that likely play a role in DC function.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
A. Grolleau-Julius, E. K. Harning, L. M. Abernathy, and R. L. Yung
Impaired Dendritic Cell Function in Aging Leads to Defective Antitumor Immunity
Cancer Res., August 1, 2008; 68(15): 6341 - 6349.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. P. Hubner, B. Pasche, S. Kalaydjiev, P. T. Soboslay, A. Lengeling, H. Schulz-Key, E. Mitre, and W. H. Hoffmann
Microfilariae of the Filarial Nematode Litomosoides sigmodontis Exacerbate the Course of Lipopolysaccharide-Induced Sepsis in Mice
Infect. Immun., April 1, 2008; 76(4): 1668 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Lehtonen, H. Ahlfors, V. Veckman, M. Miettinen, R. Lahesmaa, and I. Julkunen
Gene expression profiling during differentiation of human monocytes to macrophages or dendritic cells
J. Leukoc. Biol., September 1, 2007; 82(3): 710 - 720.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Finocchiaro, F. M. Mancuso, D. Cittaro, and H. Muller
Graph-based identification of cancer signaling pathways from published gene expression signatures using PubLiME
Nucleic Acids Res., April 1, 2007; (2007) gkm119v2.
[Abstract] [Full Text] [PDF]

Home page
 Journals of Gerontology Series A: Biological Sciences and Medical SciencesHome page
A. Grolleau-Julius, M. R. Garg, R. Mo, L. L. Stoolman, and R. L. Yung
Effect of Aging on Bone Marrow-Derived Murine CD11c+CD4-CD8{alpha}- Dendritic Cell Function.
J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2006; 61(10): 1039 - 1047.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Jozefowski, M. Arredouani, T. Sulahian, and L. Kobzik
Disparate Regulation and Function of the Class A Scavenger Receptors SR-AI/II and MARCO
J. Immunol., December 15, 2005; 175(12): 8032 - 8041.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Shell, C. Hesse, S. M. Morris Jr., and C. Milcarek
Elevated Levels of the 64-kDa Cleavage Stimulatory Factor (CstF-64) in Lipopolysaccharide-stimulated Macrophages Influence Gene Expression and Induce Alternative Poly(A) Site Selection
J. Biol. Chem., December 2, 2005; 280(48): 39950 - 39961.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Lehtonen, V. Veckman, T. Nikula, R. Lahesmaa, L. Kinnunen, S. Matikainen, and I. Julkunen
Differential Expression of IFN Regulatory Factor 4 Gene in Human Monocyte-Derived Dendritic Cells and Macrophages
J. Immunol., November 15, 2005; 175(10): 6570 - 6579.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. Ito, Q. Li, A. B. Shreiner, R. Okuyama, M. N. Jure-Kunkel, S. Teitz-Tennenbaum, and A. E. Chang
Anti-CD137 Monoclonal Antibody Administration Augments the Antitumor Efficacy of Dendritic Cell-Based Vaccines
Cancer Res., November 15, 2004; 64(22): 8411 - 8419.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.