| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, and
Diabetes Research Laboratories, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129;
Division of Rheumatology, Immunology, and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115; and
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
CD1d-restricted T cells (NKT cells) are innate memory cells activated by lipid Ags and play important roles in the initiation and regulation of the immune response. However, little is known about the trafficking patterns of these cells or the tissue compartment in which they exert their regulatory activity. In this study, we determined the chemokine receptor profile expressed by CD1d-restricted T cells found in the peripheral blood of healthy volunteers as well as CD1d-restricted T cell clones. CD1d-restricted T cells were identified by Abs recognizing the invariant V
24 TCR rearrangement or by binding to CD1d-Fc fusion tetramers loaded with -GalCer. CD1d-restricted T cells in the peripheral blood and CD1d-restricted T cell clones expressed high levels of CXCR3, CCR5, and CCR6; intermediate levels of CXCR4 and CXCR6; and low levels of CXCR1, CCR1, CCR2, and CX3CR1, a receptor pattern often associated with tissue-infiltrating effector Th1 cells and CD8+ T cells. Very few of these cells expressed the lymphoid-homing receptors CCR7 or CXCR5. CCR4 was expressed predominantly on CD4+, but not on double-negative CD1d-restricted T cells, which may indicate differential trafficking patterns for these two functionally distinct subsets. CD1d-restricted T cell clones responded to chemokine ligands for CXCR1/2, CXCR3, CXCR4, CXCR6, CCR4, and CCR5 in calcium flux and/or chemotaxis assays. These data indicate that CD1d-restricted T cells express a chemokine receptor profile most similar to Th1 inflammatory homing cells and suggest that these cells perform their function in peripheral tissue sites rather than in secondary lymphoid organs.
This article has been cited by other articles:
|
|
 |
|
  R. Cullen, E. Germanov, T. Shimaoka, and B. Johnston Enhanced Tumor Metastasis in Response to Blockade of the Chemokine Receptor CXCR6 Is Overcome by NKT Cell Activation J. Immunol., November 1, 2009; 183(9): 5807 - 5815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hegde, E. Jankowska-Gan, D. A. Roenneburg, J. Torrealba, W. J. Burlingham, and J. E. Gumperz Human NKT cells promote monocyte differentiation into suppressive myeloid antigen-presenting cells J. Leukoc. Biol., October 1, 2009; 86(4): 757 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bricard, V. Cesson, E. Devevre, H. Bouzourene, C. Barbey, N. Rufer, J. S. Im, P. M. Alves, O. Martinet, N. Halkic, et al. Enrichment of Human CD4+ V{alpha}24/V{beta}11 Invariant NKT Cells in Intrahepatic Malignant Tumors J. Immunol., April 15, 2009; 182(8): 5140 - 5151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Germanov, L. Veinotte, R. Cullen, E. Chamberlain, E. C. Butcher, and B. Johnston Critical Role for the Chemokine Receptor CXCR6 in Homeostasis and Activation of CD1d-Restricted NKT Cells J. Immunol., July 1, 2008; 181(1): 81 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Allende, D. Zhou, D. N. Kalkofen, S. Benhamed, G. Tuymetova, C. Borowski, A. Bendelac, and R. L. Proia S1P1 receptor expression regulates emergence of NKT cells in peripheral tissues FASEB J, January 1, 2008; 22(1): 307 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sakuishi, S. Oki, M. Araki, S. A. Porcelli, S. Miyake, and T. Yamamura Invariant NKT Cells Biased for IL-5 Production Act as Crucial Regulators of Inflammation J. Immunol., September 15, 2007; 179(6): 3452 - 3462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tohyama, K. Sayama, H. Komatsuzawa, Y. Hanakawa, Y. Shirakata, X. Dai, L. Yang, S. Tokumaru, H. Nagai, S. Hirakawa, et al. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes Int. Immunol., September 1, 2007; 19(9): 1095 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Thomas, A. Banerji, B. D. Medoff, C. M. Lilly, and A. D. Luster Multiple Chemokine Receptors, Including CCR6 and CXCR3, Regulate Antigen-Induced T Cell Homing to the Human Asthmatic Airway J. Immunol., August 1, 2007; 179(3): 1901 - 1912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Chang, J.-R. Huang, Y.-C. Tsai, J.-T. Hung, D. Wu, M. Fujio, C.-H. Wong, and A. L. Yu Potent immune-modulating and anticancer effects of NKT cell stimulatory glycolipids PNAS, June 19, 2007; 104(25): 10299 - 10304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hojo, K. Koizumi, K. Tsuneyama, Y. Arita, Z. Cui, K. Shinohara, T. Minami, I. Hashimoto, T. Nakayama, H. Sakurai, et al. High-Level Expression of Chemokine CXCL16 by Tumor Cells Correlates with a Good Prognosis and Increased Tumor-Infiltrating Lymphocytes in Colorectal Cancer Cancer Res., May 15, 2007; 67(10): 4725 - 4731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Im, N. Tapinos, G.-T. Chae, P. A. Illarionov, G. S. Besra, G. H. DeVries, R. L. Modlin, P. A. Sieling, A. Rambukkana, and S. A. Porcelli Expression of CD1d Molecules by Human Schwann Cells and Potential Interactions with Immunoregulatory Invariant NK T Cells J. Immunol., October 15, 2006; 177(8): 5226 - 5235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Horikawa, M. Fujimoto, M. Hasegawa, T. Matsushita, Y. Hamaguchi, A. Kawasuji, Y. Matsushita, T. Fujita, F. Ogawa, K. Takehara, et al. E- and P-Selectins Synergistically Inhibit Bleomycin-Induced Pulmonary Fibrosis Am. J. Pathol., September 1, 2006; 169(3): 740 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Colvin, G. S. V. Campanella, L. A. Manice, and A. D. Luster CXCR3 Requires Tyrosine Sulfation for Ligand Binding and a Second Extracellular Loop Arginine Residue for Ligand-Induced Chemotaxis Mol. Cell. Biol., August 1, 2006; 26(15): 5838 - 5849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lin, M. Nieda, J. F. Hutton, V. Rozenkov, and A. J. Nicol Comparative gene expression analysis of NKT cell subpopulations J. Leukoc. Biol., July 1, 2006; 80(1): 164 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Thomas, C. M. Lilly, A. D. Luster, N. Pham-Thi, J. de Blic, M. C. Leite-de-Moraes, O. Akbari, J. L. Faul, and D. T. Umetsu Invariant natural killer T cells in bronchial asthma. N. Engl. J. Med., June 15, 2006; 354(24): 2613 - 2616. [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Matsuda, Q. Zhang, R. Ndonye, S. K. Richardson, A. R. Howell, and L. Gapin T-bet concomitantly controls migration, survival, and effector functions during the development of V{alpha}14i NKT cells Blood, April 1, 2006; 107(7): 2797 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Islam, S. Y. Thomas, C. Hess, B. D. Medoff, T. K. Means, C. Brander, C. M. Lilly, A. M. Tager, and A. D. Luster The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans Blood, January 15, 2006; 107(2): 444 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-C. Shin, U. Protzer, A. Untergasser, S. M. Feinstone, C. M. Rice, D. Hasselschwert, and B. Rehermann Liver-Directed Gamma Interferon Gene Delivery in Chronic Hepatitis C J. Virol., November 1, 2005; 79(21): 13412 - 13420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sen, B. Yongyi, H. Yuling, X. Luokun, H. Li, X. Jie, D. Tao, Z. Gang, L. Junyan, H. Chunsong, et al. V{alpha}24-Invariant NKT Cells from Patients with Allergic Asthma Express CCR9 at High Frequency and Induce Th2 Bias of CD3+ T Cells upon CD226 Engagement J. Immunol., October 15, 2005; 175(8): 4914 - 4926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Ajuebor, A. I. Aspinall, F. Zhou, T. Le, Y. Yang, S. J. Urbanski, S. Sidobre, M. Kronenberg, C. M. Hogaboam, and M. G. Swain Lack of Chemokine Receptor CCR5 Promotes Murine Fulminant Liver Failure by Preventing the Apoptosis of Activated CD1d-Restricted NKT Cells J. Immunol., June 15, 2005; 174(12): 8027 - 8037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tabata, N. Kadowaki, T. Kitawaki, T. Shimaoka, S. Yonehara, O. Yoshie, and T. Uchiyama Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells J. Leukoc. Biol., May 1, 2005; 77(5): 777 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Jie, Z Pan, Y Chen, Y Wei, W Zhang, Y Wu, H Peng, and L Xu Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats Br J Ophthalmol, March 1, 2005; 89(3): 364 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Oh, S. Kim, S.-H. Park, H. Gu, D. Roopenian, D. H. Chung, Y. S. Kim, and D.-S. Lee Direct Regulatory Role of NKT Cells in Allogeneic Graft Survival Is Dependent on the Quantitative Strength of Antigenicity J. Immunol., February 15, 2005; 174(4): 2030 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Broderick, S. J. Yokota, J. Reineke, E. Mathiowitz, C. C. Stewart, M. Barcos, R. J. Kelleher Jr., and R. B. Bankert Human CD4+ Effector Memory T Cells Persisting in the Microenvironment of Lung Cancer Xenografts Are Activated by Local Delivery of IL-12 to Proliferate, Produce IFN-{gamma}, and Eradicate Tumor Cells J. Immunol., January 15, 2005; 174(2): 898 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Baev, X.-h. Peng, L. Song, J. R. Barnhart, G. M. Crooks, K. I. Weinberg, and L. S. Metelitsa Distinct homeostatic requirements of CD4+ and CD4- subsets of V{alpha}24-invariant natural killer T cells in humans Blood, December 15, 2004; 104(13): 4150 - 4156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lavergne, C. Combadiere, M. Iga, A. Boissonnas, O. Bonduelle, M. Maho, P. Debre, and B. Combadiere Intratumoral CC Chemokine Ligand 5 Overexpression Delays Tumor Growth and Increases Tumor Cell Infiltration J. Immunol., September 15, 2004; 173(6): 3755 - 3762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Metelitsa, H.-W. Wu, H. Wang, Y. Yang, Z. Warsi, S. Asgharzadeh, S. Groshen, S. B. Wilson, and R. C. Seeger Natural Killer T Cells Infiltrate Neuroblastomas Expressing the Chemokine CCL2 J. Exp. Med., May 3, 2004; 199(9): 1213 - 1221. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
