The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ogilvy, S.
Right arrow Articles by Holmes, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogilvy, S.
Right arrow Articles by Holmes, N.
The Journal of Immunology, 2003, 171: 1792-1800.
Copyright © 2003 by The American Association of Immunologists

Either of the CD45RB and CD45RO Isoforms Are Effective in Restoring T Cell, But Not B Cell, Development and Function in CD45-Null Mice1

Sarah Ogilvy2,*, Christine Louis-Dit-Sully3,{dagger}, Joanne Cooper*, Robin L. Cassady*, Denis R. Alexander* and Nick Holmes4,{dagger}

* Laboratory of Lymphocyte Signaling and Development, The Babraham Institute, Babraham, Cambridge, United Kingdom; and {dagger} Department of Pathology, University of Cambridge, Cambridge, United Kingdom

The protein tyrosine phosphatase CD45 is expressed as a series of isoforms whose tissue and differentiation stage specificity is broadly conserved in evolution. CD45 has been shown to be an important regulator of a variety of functions in many different hemopoietic lineages. We have chosen an in vivo genetic complementation strategy to investigate the differential functions between isoforms. In this study, we report the characterization of transgenic mice which express the isoforms CD45RO or CD45RB as their only CD45 molecules, at a variety of expression levels and in the majority of hemopoietic lineages. Both CD45RO and CD45RB isoforms reconstitute thymocyte development in a CD45-null mouse background when expressed above a threshold level. The resulting mature T cells populate the peripheral lymphoid organs where they are found at normal frequency. Both CD45RO and CD45RB isoforms also permit T cell function in the periphery, although the threshold for normal function here appears to be set higher than in the thymus. In contrast, neither isoform is capable of fully restoring peripheral B cell maturation, even at levels approaching those in heterozygous CD45+/- mice in which maturation is normal. In vitro activation of B cells by Ag-receptor stimulation is only minimally complemented by these CD45RO and CD45RB transgenes. Our results suggest that CD45 isoforms play unique roles which differ between the T and B lineages.




This article has been cited by other articles:


Home page
 BloodHome page
K. Parikh, S. Poppema, M. P. Peppelenbosch, and L. Visser
Extracellular ligation-dependent CD45RB enzymatic activity negatively regulates lipid raft signal transduction
Blood, January 15, 2009; 113(3): 594 - 603.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. J. Salmond, L. McNeill, N. Holmes, and D. R. Alexander
CD4+ T cell hyper-responsiveness in CD45 transgenic mice is independent of isoform
Int. Immunol., July 1, 2008; 20(7): 819 - 827.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. M. Jackson, N. Harp, D. Patel, J. Zhang, S. Willson, Y. J. Kim, C. Clanton, and J. D. Capra
CD45RO enriches for activated, highly mutated human germinal center B cells
Blood, December 1, 2007; 110(12): 3917 - 3925.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
T. Moroy and F. Heyd
The impact of alternative splicing in vivo: Mouse models show the way
RNA, August 1, 2007; 13(8): 1155 - 1171.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. R. J. Taylor, D. R. Alexander, J. C. Cooper, C. F. Higgins, and J. I. Elliott
Regulatory T Cells Are Resistant to Apoptosis via TCR but Not P2X7
J. Immunol., March 15, 2007; 178(6): 3474 - 3482.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. I. Elliott, A. Sardini, J. C. Cooper, D. R. Alexander, S. Davanture, G. Chimini, and C. F. Higgins
Phosphatidylserine exposure in B lymphocytes: a role for lipid packing
Blood, September 1, 2006; 108(5): 1611 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Dawes, S. Petrova, Z. Liu, D. Wraith, P. C. L. Beverley, and E. Z. Tchilian
Combinations of CD45 Isoforms Are Crucial for Immune Function and Disease
J. Immunol., March 15, 2006; 176(6): 3417 - 3425.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
M. C. Lopez and N. Holmes
Phenotypical and functional alterations in the mucosal immune system of CD45 exon 9 KO mice
Int. Immunol., January 1, 2005; 17(1): 15 - 25.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. Boxall, T. Stanton, K. Hirai, V. Ward, T. Yasui, H. Tahara, A. Tamori, S. Nishiguchi, S. Shiomi, O. Ishiko, et al.
Disease associations and altered immune function in CD45 138G variant carriers
Hum. Mol. Genet., October 1, 2004; 13(20): 2377 - 2384.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
E. Z. Tchilian, R. Dawes, L. Hyland, M. Montoya, A. Le Bon, P. Borrow, S. Hou, D. Tough, and P. C. L. Beverley
Altered CD45 isoform expression affects lymphocyte function in CD45 Tg mice
Int. Immunol., September 1, 2004; 16(9): 1323 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. E. Fleming, C. D. Milne, and C. J. Paige
CD45-Deficient Mice Accumulate Pro-B Cells Both In Vivo and In Vitro
J. Immunol., August 15, 2004; 173(4): 2542 - 2551.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.