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Antibody Repertoire and Gene Expression Profile: Implications for Different Developmental and Functional Traits of Splenic and Peritoneal B-1 Lymphocytes ¹

Karsten Kretschmer^2,*, Anke Jungebloud^*, Jana Stopkowicz^*, Britta Stoermann^*, Reinhard Hoffmann^3, and Siegfried Weiss^*

^* Molecular Immunology, German Research Center for Biotechnology, Braunschweig, Germany; and Basel Institute for Immunology, Basel, Switzerland

In L2 mice, a high expression level of the transgenic 2³¹⁵ L chain results in nearly complete exclusion of endogenous L chains and a predominance of B-1a cells. In this study, we show that splenic and peritoneal B-1a cells differ considerably in their Ab repertoire and gene expression profile. Splenic B-1a cells exhibit a more diversified repertoire under L chain limitation. Despite oligoclonal overlaps between both B-1a compartments, some B cell receptor specificities are clearly restricted to the peritoneum. The capacity of peritoneal B-1a cells to enter the splenic B-1a compartment was found to be very limited. Gene expression profiling revealed genes up-regulated in splenic B-1a cells that are involved in mediating specialized first-line-of-defense effector functions and interaction with T cells. Thus, splenic and peritoneal B-1a cells differ not only in their developmental program but also in functional properties.

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