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CD49d Overexpression and T Cell Autoimmunity¹

Ru-Ran Mo^*, Julie K. Eisenbraun^*, Joanne Sonstein, Ronald A. Craig, Jeffrey L. Curtis, Lloyd M. Stoolman, Jun Chen^* and Raymond L. Yung^2,*,

Divisions of ^* Geriatric Medicine and Rheumatology and Pulmonary and Critical Care Medicine, Department of Internal Medicine, and Department of Pathology, University of Michigan, and Geriatrics Research, Education, and Clinical Center, Ann Arbor Veterans Affairs Health System, Ann Arbor, MI 48109

D10.G4.1 (D10) cells, a murine conalbumin-reactive Th2 cell line, made to overexpress the ₂ integrin LFA-1 by pharmacological manipulation or by transfection become autoreactive and are capable of inducing in vivo autoimmunity. However, whether this is specific to LFA-1 and whether overexpression of other T cell integrin molecules has the same effect are unknown. We examined the functional consequences of T cell CD49d (₄ integrin) overexpression by transfecting murine CD49d cDNA into D10 cells. Similar to the LFA-1-transfected cells, the CD49d-overexpressing T cells are autoreactive and proliferate in response to APCs in an MHC class II-dependent manner in the absence of nominal Ag. Additionally, CD49d overexpression is associated with increased in vitro adhesion to endothelial cells and increased in vivo splenic homing. However, in contrast to LFA-1 overexpression, increased T cell CD49d expression is not associated with autoreactive cytotoxicity or the ability to induce in vivo autoimmunity. In addition to the novel observation that CD49d overexpression is sufficient to induce T cell autoreactivity, our results also support the hypothesis that the ability to induce in vivo autoimmunity is related to T cell cytotoxicity and not to T cell proliferation function in the D10 murine adoptive transfer model of autoimmunity.

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