| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
, but Not by TNF-
1
Departments of
*
Hematology and
Pathology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Synthesis of the antimicrobial protein neutrophil gelatinase-associated lipocalin (NGAL) increases dramatically in bronchial epithelial cells and alveolear type II pneumocytes during lung inflammation. IL-1
induces a >10-fold up-regulation of NGAL expression in the type II pneumocyte-derived cell line A549 cells, whereas TNF-
, IL-6, and LPS had no effect. Similar IL-1 selectivity was demonstrated in primary bronchial epithelial cells and epidermal keratinocytes and for an NGAL promoter fragment transfected into A549 cells. By deletion and substitution analysis of the NGAL promoter, a 40-bp region containing an NF-
B consensus site was found to control the IL-1-specific up-regulation. Involvement of the NF-B site was demonstrated by site-directed mutagenesis, by transfection with a dominant-negative inhibitor of the NF-B pathway, and by EMSA. TNF- activation of NF-B, in contrast, did not increase NGAL synthesis, even though induced binding of NF-B to the NGAL promoter was observed in vitro. IL-1 specificity was not contained within the NF-B site of the NGAL promoter, as determined by exchanging the NGAL promoter's NF-B-binding sequence with that of the IL-8 promoter or with the NF-B consensus sequence and by testing the NF-B-binding sequence of the NGAL promoter against the heterologous SV40 promoter. Selectivity for the IL-1 pathway was substantiated by demonstrating that NGAL promoter activity could be induced by LPS stimulation of A549 cells transiently expressing Toll-like receptor 4, which use the same intracellular signaling pathway as the IL-1R. Together, this demonstrates a selective up-regulation of NGAL by the IL-1 pathway.
This article has been cited by other articles:
|
|
 |
|
  K. Vardarova, S. Scharf, F. Lang, B. Schmeck, B. Opitz, J. Eitel, A. C. Hocke, H. Slevogt, A. Flieger, S. Hippenstiel, et al. PKC{alpha} and PKC{epsilon} differentially regulate Legionella pneumophila-induced GM-CSF Eur. Respir. J., November 1, 2009; 34(5): 1171 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, J.-Y. Park, W.-H. Lee, H. Kim, H.-C. Park, K. Mori, and K. Suk Lipocalin-2 Is an Autocrine Mediator of Reactive Astrocytosis J. Neurosci., January 7, 2009; 29(1): 234 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yamazaki, S. Matsuo, T. Muta, M. Yamamoto, S. Akira, and K. Takeshige Gene-specific Requirement of a Nuclear Protein, I{kappa}B-{zeta}, for Promoter Association of Inflammatory Transcription Regulators J. Biol. Chem., November 21, 2008; 283(47): 32404 - 32411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Woo, J.-K. Min, C. H. Bai, S.-Y. Song, H. J. Kang, H.-M. Lee, and Y.-D. Kim Expression of Neutrophil Gelatinase-Associated Lipocalin in Nasal Polyps Arch Otolaryngol Head Neck Surg, November 1, 2008; 134(11): 1182 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Iannetti, F. Pacifico, R. Acquaviva, A. Lavorgna, E. Crescenzi, C. Vascotto, G. Tell, A. M. Salzano, A. Scaloni, E. Vuttariello, et al. The neutrophil gelatinase-associated lipocalin (NGAL), a NF-{kappa}B-regulated gene, is a survival factor for thyroid neoplastic cells PNAS, September 16, 2008; 105(37): 14058 - 14063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, J. Lee, S. Kim, J.-Y. Park, W.-H. Lee, K. Mori, S.-H. Kim, I. K. Kim, and K. Suk A Dual Role of Lipocalin 2 in the Apoptosis and Deramification of Activated Microglia J. Immunol., September 1, 2007; 179(5): 3231 - 3241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Nelson, A. J. Ratner, J. Barasch, and J. N. Weiser Interleukin-8 Secretion in Response to Aferric Enterobactin Is Potentiated by Siderocalin Infect. Immun., June 1, 2007; 75(6): 3160 - 3168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, L. Xu, D. Xiao, J. Xie, H. Zeng, Z. Wang, X. Zhang, Y. Niu, Z. Shen, J. Shen, et al. Upregulation of neutrophil gelatinase-associated lipocalin in oesophageal squamous cell carcinoma: significant correlation with cell differentiation and tumour invasion J. Clin. Pathol., May 1, 2007; 60(5): 555 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-x. Bu, A.-L. Hemdahl, A. Gabrielsen, J. Fuxe, C. Zhu, P. Eriksson, and Z.-q. Yan Induction of Neutrophil Gelatinase-Associated Lipocalin in Vascular Injury via Activation of Nuclear Factor-{kappa}B Am. J. Pathol., December 1, 2006; 169(6): 2245 - 2253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Shen, Z. Hu, J. Goswami, and S. L. Gaffen Identification of Common Transcriptional Regulatory Elements in Interleukin-17 Target Genes J. Biol. Chem., August 25, 2006; 281(34): 24138 - 24148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Andre, T. Stoeger, S. Takenaka, M. Bahnweg, B. Ritter, E. Karg, B. Lentner, C. Reinhard, H. Schulz, and M. Wjst Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung Eur. Respir. J., August 1, 2006; 28(2): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Y. Lee, M.-K. Kim, K. S. Park, E. H. Shin, S. H. Jo, S. D. Kim, E. J. Jo, Y.-N. Lee, C. Lee, S.-H. Baek, et al. Serum Amyloid A Induces Contrary Immune Responses via Formyl Peptide Receptor-Like 1 in Human Monocytes Mol. Pharmacol., July 1, 2006; 70(1): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Cowland, T. Muta, and N. Borregaard IL-1beta-Specific Up-Regulation of Neutrophil Gelatinase-Associated Lipocalin Is Controlled by I{kappa}B-{zeta} J. Immunol., May 1, 2006; 176(9): 5559 - 5566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-S. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, H. Kogo, and T. Hara Spermatogonial Cell-Mediated Activation of an I{kappa}B{zeta}-Independent Nuclear Factor-{kappa}B Pathway in Sertoli Cells Induces Transcription of the Lipocalin-2 Gene Mol. Endocrinol., April 1, 2006; 20(4): 904 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Fernandez, L. Yan, G. Louis, J. Yang, J. L. Kutok, and M. A. Moses The Matrix Metalloproteinase-9/Neutrophil Gelatinase-Associated Lipocalin Complex Plays a Role in Breast Tumor Growth and Is Present in the Urine of Breast Cancer Patients Clin. Cancer Res., August 1, 2005; 11(15): 5390 - 5395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Borregaard, K. Theilgaard-Monch, J. B. Cowland, M. Stahle, and O. E. Sorensen Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place J. Leukoc. Biol., April 1, 2005; 77(4): 439 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Gwira, F. Wei, S. Ishibe, J. M. Ueland, J. Barasch, and L. G. Cantley Expression of Neutrophil Gelatinase-associated Lipocalin Regulates Epithelial Morphogenesis in Vitro J. Biol. Chem., March 4, 2005; 280(9): 7875 - 7882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamazaki, T. Muta, S. Matsuo, and K. Takeshige Stimulus-specific Induction of a Novel Nuclear Factor-{kappa}B Regulator, I{kappa}B-{zeta}, via Toll/Interleukin-1 Receptor Is Mediated by mRNA Stabilization J. Biol. Chem., January 14, 2005; 280(2): 1678 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gerhardinger, M. B. Costa, M. C. Coulombe, I. Toth, T. Hoehn, and P. Grosu Expression of Acute-Phase Response Proteins in Retinal Muller Cells in Diabetes Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 349 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Jo, H.-Y. Lee, Y.-N. Lee, J. I. Kim, H.-K. Kang, D.-W. Park, S.-H. Baek, J.-Y. Kwak, and Y.-S. Bae Group IB Secretory Phospholipase A2 Stimulates CXC Chemokine Ligand 8 Production via ERK and NF-{kappa}B in Human Neutrophils J. Immunol., November 15, 2004; 173(10): 6433 - 6439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
