| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Induced Inflammation 1
* Department of Biomedical Engineering and
Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908; and Departments of
Pediatrics,
Medicine, and
¶ Human Genetics, Baylor College of Medicine, Houston, TX 77030
We have previously shown that Mac-1 and LFA-1 play a cooperative role in slow leukocyte rolling in inflamed vessels, and that, although both have a role in leukocyte adhesion, the contribution from LFA-1 exceeds that of Mac-1. In this study, we used mice deficient in ICAM-1 (ICAM-1null) to study the function of ICAM-1 as an endothelial ligand for Mac-1 and LFA-1. The cremaster muscles of these mice were treated with TNF-
and prepared for intravital microscopy. We found that the average rolling velocity in venules was not different in ICAM-1null mice (4.7 µm/s) compared with wild-type mice (5.1 µm/s). Similarly, leukocyte adhesion efficiency in ICAM-1null mice (0.11 ± 0.01 mm) was similar to that in Mac-1-/- (0.12 ± 0.03 mm) mice but significantly increased compared with that in LFA-1-/- (0.08 ± 0.01 mm) mice and significantly reduced from that in wild type (0.26 ± 0.04 mm). When both LFA-1 and ICAM-1 were blocked, rolling velocity increased, and adhesion efficiency and arrest decreased. However, blocking both Mac-1 and ICAM-1 had no greater effect than either blockade alone. We conclude that endothelial ICAM-1 is the main ligand responsible for slow leukocyte rolling mediated by Mac-1, but not LFA-1.
This article has been cited by other articles:
|
|
 |
|
  L. Piqueras, M. J. Sanz, M. Perretti, E. Morcillo, L. Norling, J. A. Mitchell, Y. Li, and D. Bishop-Bailey Activation of PPAR{beta}/{delta} inhibits leukocyte recruitment, cell adhesion molecule expression, and chemokine release J. Leukoc. Biol., July 1, 2009; 86(1): 115 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Woollard, A. Suhartoyo, E. E. Harris, S. U. Eisenhardt, S. P. Jackson, K. Peter, A. M. Dart, M. J. Hickey, and J. P.F. Chin-Dusting Pathophysiological Levels of Soluble P-Selectin Mediate Adhesion of Leukocytes to the Endothelium Through Mac-1 Activation Circ. Res., November 7, 2008; 103(10): 1128 - 1138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yakubenia, D. Frommhold, D. Scholch, C. C. Hellbusch, C. Korner, B. Petri, C. Jones, U. Ipe, M. G. Bixel, R. Krempien, et al. Leukocyte trafficking in a mouse model for leukocyte adhesion deficiency II/congenital disorder of glycosylation IIc Blood, August 15, 2008; 112(4): 1472 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lauterbach, P. O'Donnell, K. Asano, and T. N. Mayadas Role of TNF priming and adhesion molecules in neutrophil recruitment to intravascular immune complexes J. Leukoc. Biol., June 1, 2008; 83(6): 1423 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shimizu, P. Libby, R. Shubiki, M. Sakuma, Y. Wang, K. Asano, R. N. Mitchell, and D. I. Simon Leukocyte Integrin Mac-1 Promotes Acute Cardiac Allograft Rejection Circulation, April 15, 2008; 117(15): 1997 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Hom, R. R. Graham, B. Modrek, K. E. Taylor, W. Ortmann, S. Garnier, A. T. Lee, S. A. Chung, R. C. Ferreira, P.V. K. Pant, et al. Association of Systemic Lupus Erythematosus with C8orf13-BLK and ITGAM-ITGAX N. Engl. J. Med., February 28, 2008; 358(9): 900 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sumagin and I. H. Sarelius A role for ICAM-1 in maintenance of leukocyte-endothelial cell rolling interactions in inflamed arterioles Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2786 - H2798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wu, R. Guo, Y. Wang, and P. N. Cunningham The role of ICAM-1 in endotoxin-induced acute renal failure Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1262 - F1271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Sullivan, A. B. M. Reddy, K. Dietzmann, A. R. Suriano, V. P. Kocieda, M. Stewart, and M. Bhatia Epigenetic Regulation of Tumor Necrosis Factor Alpha Mol. Cell. Biol., July 15, 2007; 27(14): 5147 - 5160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Park, M. Amin Arnaout, and V. Gupta A Simple, No-Wash Cell Adhesion-Based High-Throughput Assay for the Discovery of Small-Molecule Regulators of the Integrin CD11b/CD18 J Biomol Screen, April 1, 2007; 12(3): 406 - 417. [Abstract] [PDF]
|
|
|
|
 |
|
 D. C. Bullard, X. Hu, T. R. Schoeb, R. G. Collins, A. L. Beaudet, and S. R. Barnum Intercellular Adhesion Molecule-1 Expression Is Required on Multiple Cell Types for the Development of Experimental Autoimmune Encephalomyelitis J. Immunol., January 15, 2007; 178(2): 851 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Pello, B. Duthey, D. Garcia-Bernal, J. M. Rodriguez-Frade, J. V. Stein, J. Teixido, C. Martinez-A., and M. Mellado Opioids Trigger {alpha}5beta1 Integrin-Mediated Monocyte Adhesion J. Immunol., February 1, 2006; 176(3): 1675 - 1685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Bonder, M. U. Norman, T. MacRae, P. R. Mangan, C. T. Weaver, D. C. Bullard, D.-M. McCafferty, and P. Kubes P-Selectin Can Support Both Th1 and Th2 Lymphocyte Rolling in the Intestinal Microvasculature Am. J. Pathol., December 1, 2005; 167(6): 1647 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xu, W. Xu, Y. Chu, Y. Gong, Z. Jiang, and S. Xiong Involvement of Up-Regulated CXC Chemokine Ligand 16/Scavenger Receptor That Binds Phosphatidylserine and Oxidized Lipoprotein in Endotoxin-Induced Lethal Liver Injury via Regulation of T-Cell Recruitment and Adhesion Infect. Immun., July 1, 2005; 73(7): 4007 - 4016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Liu, S. A. Caldwell, and S. I. Abrams Cooperative Disengagement of Fas and Intercellular Adhesion Molecule-1 Function in Neoplastic Cells Confers Enhanced Colonization Efficiency Cancer Res., February 1, 2005; 65(3): 1045 - 1054. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
