| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Immunobiology Laboratory and
Computational Genome Analysis Laboratory, Cancer Research U.K., London Research Institute, London, United Kingdom; and
Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
The functional relationships and properties of different subtypes of dendritic cells (DC) remain largely undefined. To better characterize these cells, we used global gene analysis to determine gene expression patterns among murine CD11chigh DC subsets. CD4+, CD8
+, and CD8- CD4- (double negative (DN)) DC were purified from spleens of normal C57/BL6 mice and analyzed using Affymetrix microarrays. The CD4+ and CD8+ DC subsets showed distinct basal expression profiles differing by >200 individual genes. These included known DC subset markers as well as previously unrecognized, differentially expressed CD Ags such as CD1d, CD5, CD22, and CD72. Flow cytometric analysis confirmed differential expression in nine of nine cases, thereby validating the microarray analysis. Interestingly, the microarray expression profiles for DN cells strongly resembled those of CD4+ DC, differing from them by <25 genes. This suggests that CD4+ and DN DC are closely related phylogenetically, whereas CD8+ DC represent a more distant lineage, supporting the historical distinction between CD8+ and CD8- DC. However, staining patterns revealed that in contrast to CD4+ DC, the DN subset is heterogeneous and comprises at least two subpopulations. Gene Ontology and literature mining analyses of genes expressed differentially among DC subsets indicated strong associations with immune response parameters as well as cell differentiation and signaling. Such associations offer clues to possible unique functions of the CD11chigh DC subsets that to date have been difficult to define as rigid distinctions.
This article has been cited by other articles:
|
|
 |
|
  M. V. Lukens, D. Kruijsen, F. E. J. Coenjaerts, J. L. L. Kimpen, and G. M. van Bleek Respiratory Syncytial Virus-Induced Activation and Migration of Respiratory Dendritic Cells and Subsequent Antigen Presentation in the Lung-Draining Lymph Node J. Virol., July 15, 2009; 83(14): 7235 - 7243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Qiu, Y. Miyake, H. Kaise, H. Kitamura, O. Ohara, and M. Tanaka Novel Subset of CD8{alpha}+ Dendritic Cells Localized in the Marginal Zone Is Responsible for Tolerance to Cell-Associated Antigens J. Immunol., April 1, 2009; 182(7): 4127 - 4136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Gerner and M. F. Mescher Antigen Processing and MHC-II Presentation by Dermal and Tumor-Infiltrating Dendritic Cells J. Immunol., March 1, 2009; 182(5): 2726 - 2737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hildner, B. T. Edelson, W. E. Purtha, M. Diamond, H. Matsushita, M. Kohyama, B. Calderon, B. U. Schraml, E. R. Unanue, M. S. Diamond, et al. Batf3 Deficiency Reveals a Critical Role for CD8{alpha}+ Dendritic Cells in Cytotoxic T Cell Immunity Science, November 14, 2008; 322(5904): 1097 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Caminschi, A. I. Proietto, F. Ahmet, S. Kitsoulis, J. Shin Teh, J. C. Y. Lo, A. Rizzitelli, L. Wu, D. Vremec, S. L. H. van Dommelen, et al. The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement Blood, October 15, 2008; 112(8): 3264 - 3273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Y. Taraban, S. Martin, K. E. Attfield, M. J. Glennie, T. Elliott, D. Elewaut, S. Van Calenbergh, B. Linclau, and A. Al-Shamkhani Invariant NKT Cells Promote CD8+ Cytotoxic T Cell Responses by Inducing CD70 Expression on Dendritic Cells J. Immunol., April 1, 2008; 180(7): 4615 - 4620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Tailor, T. Tamura, H. C. Morse III, and K. Ozato The BXH2 mutation in IRF8 differentially impairs dendritic cell subset development in the mouse Blood, February 15, 2008; 111(4): 1942 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Giroux, E. Yurchenko, J. St.-Pierre, C. A. Piccirillo, and C. Perreault T Regulatory Cells Control Numbers of NK Cells and CD8{alpha}+ Immature Dendritic Cells in the Lymph Node Paracortex J. Immunol., October 1, 2007; 179(7): 4492 - 4502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Segura, C. Guerin, N. Hogg, S. Amigorena, and C. Thery CD8+ Dendritic Cells Use LFA-1 to Capture MHC-Peptide Complexes from Exosomes In Vivo J. Immunol., August 1, 2007; 179(3): 1489 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Skokos and M. C. Nussenzweig CD8- DCs induce IL-12-independent Th1 differentiation through Delta 4 Notch-like ligand in response to bacterial LPS J. Exp. Med., July 9, 2007; 204(7): 1525 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Caton, M. R. Smith-Raska, and B. Reizis Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen J. Exp. Med., July 9, 2007; 204(7): 1653 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Okajo, Y. Kaneko, Y. Murata, T. Tomizawa, C. Okuzawa, Y. Saito, Y. Kaneko, T. Ishikawa-Sekigami, H. Okazawa, H. Ohnishi, et al. Regulation by Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase Substrate-1 of {alpha}-Galactosylceramide-Induced Antimetastatic Activity and Th1 and Th2 Responses of NKT Cells J. Immunol., May 15, 2007; 178(10): 6164 - 6172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Durakovic, K. B. Bezak, M. Skarica, V. Radojcic, E. J. Fuchs, G. F. Murphy, and L. Luznik Host-Derived Langerhans Cells Persist after MHC-Matched Allografting Independent of Donor T Cells and Critically Influence the Alloresponses Mediated by Donor Lymphocyte Infusions J. Immunol., October 1, 2006; 177(7): 4414 - 4425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Lahoud, A. I. Proietto, K. H. Gartlan, S. Kitsoulis, J. Curtis, J. Wettenhall, M. Sofi, C. Daunt, M. O'Keeffe, I. Caminschi, et al. Signal Regulatory Protein Molecules Are Differentially Expressed by CD8- Dendritic Cells J. Immunol., July 1, 2006; 177(1): 372 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Caminschi, A. J Corbett, C. Zahra, M. Lahoud, K. M Lucas, M. Sofi, D. Vremec, T. Gramberg, S. Pohlmann, J. Curtis, et al. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN Int. Immunol., May 1, 2006; 18(5): 741 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Orabona, P. Puccetti, C. Vacca, S. Bicciato, A. Luchini, F. Fallarino, R. Bianchi, E. Velardi, K. Perruccio, A. Velardi, et al. Toward the identification of a tolerogenic signature in IDO-competent dendritic cells Blood, April 1, 2006; 107(7): 2846 - 2854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-S. J. Sung, S. M. Fu, C. E. Rose Jr., F. Gaskin, S.-T. Ju, and S. R. Beaty A Major Lung CD103 ({alpha}E)-beta7 Integrin-Positive Epithelial Dendritic Cell Population Expressing Langerin and Tight Junction Proteins J. Immunol., February 15, 2006; 176(4): 2161 - 2172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Annacker, J. L. Coombes, V. Malmstrom, H. H. Uhlig, T. Bourne, B. Johansson-Lindbom, W. W. Agace, C. M. Parker, and F. Powrie Essential role for CD103 in the T cell-mediated regulation of experimental colitis J. Exp. Med., October 17, 2005; 202(8): 1051 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Galibert, G. S. Diemer, Z. Liu, R. S. Johnson, J. L. Smith, T. Walzer, M. R. Comeau, C. T. Rauch, M. F. Wolfson, R. A. Sorensen, et al. Nectin-like Protein 2 Defines a Subset of T-cell Zone Dendritic Cells and Is a Ligand for Class-I-restricted T-cell-associated Molecule J. Biol. Chem., June 10, 2005; 280(23): 21955 - 21964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tamura, P. Tailor, K. Yamaoka, H. J. Kong, H. Tsujimura, J. J. O'Shea, H. Singh, and K. Ozato IFN Regulatory Factor-4 and -8 Govern Dendritic Cell Subset Development and Their Functional Diversity J. Immunol., March 1, 2005; 174(5): 2573 - 2581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kerksiek, F. Niedergang, P. Chavrier, D. H. Busch, and T. Brocker Selective Rac1 inhibition in dendritic cells diminishes apoptotic cell uptake and cross-presentation in vivo Blood, January 15, 2005; 105(2): 742 - 749. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
