| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
Graduate Programs in
*
Biochemistry, Microbiology, and Molecular Biology and
Pathobiology, and Departments of
Veterinary Science and
Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802
HIV-1 is an enveloped retrovirus that acquires its outer membrane as the virion exits the cell. Because of the association of apoptosis with the progression of AIDS, HIV-1-infected T cells or macrophages might be expected to express elevated levels of surface phosphatidylserine (PS), a hallmark of programmed cell death. Virions produced by these cells would also be predicted to have PS on the surface of their envelopes. In this study, data are presented that support this hypothesis and suggest that PS is required for macrophage infection. The PS-specific protein annexin V was used to enrich for virus particles and to inhibit HIV-1 replication in primary macrophages, but not T cells. HIV-1 replication was also significantly inhibited with vesicles consisting of PS, but not phosphatidylcholine. PS is specifically required for HIV-1 infection because viruses pseudotyped with vesicular stomatitis virus G and amphotropic murine leukemia virus envelopes were not inhibited by PS vesicles or annexin V. These data indicate that PS is an important cofactor for HIV-1 infection of macrophages.
This article has been cited by other articles:
|
|
 |
|
  L. E. Munoz, S. Franz, F. Pausch, B. Furnrohr, A. Sheriff, B. Vogt, P. M. Kern, W. Baum, C. Stach, D. von Laer, et al. The influence on the immunomodulatory effects of dying and dead cells of Annexin V J. Leukoc. Biol., January 1, 2007; 81(1): 6 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Wahl, T. Greenwell-Wild, and N. Vazquez HIV accomplices and adversaries in macrophage infection J. Leukoc. Biol., November 1, 2006; 80(5): 973 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. N. Gekonge, G. Schiralli, R. A. Schlegel, and A. J. Henderson Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages J. Leukoc. Biol., October 1, 2006; 80(4): 953 - 960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N.M. Moutsopoulos, T. Greenwell-Wild, and S.M. Wahl Differential Mucosal Susceptibility in HIV-1 Transmission and Infection Advances in Dental Research, April 1, 2006; 19(1): 52 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Meschi, E. C. Crouch, P. Skolnik, K. Yahya, U. Holmskov, R. Leth-Larsen, I. Tornoe, T. Tecle, M. R. White, and K. L. Hartshorn Surfactant protein D binds to human immunodeficiency virus (HIV) envelope protein gp120 and inhibits HIV replication J. Gen. Virol., November 1, 2005; 86(11): 3097 - 3107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Coil and A. D. Miller Enhancement of Enveloped Virus Entry by Phosphatidylserine J. Virol., September 1, 2005; 79(17): 11496 - 11500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. del Conde, C. N. Shrimpton, P. Thiagarajan, and J. A. Lopez Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation Blood, September 1, 2005; 106(5): 1604 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ma, T. Greenwell-Wild, K. Lei, W. Jin, J. Swisher, N. Hardegen, C. T. Wild, and S. M. Wahl Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection J. Exp. Med., November 15, 2004; 200(10): 1337 - 1346. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
