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2V
2 T Cells1
* Division of Rheumatology, Department of Internal Medicine and Interdisciplinary Group in Immunology, University of Iowa College of Medicine, Iowa City, IA 52442;
Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women’s Hospital, and
Division of Viral Pathogenesis, Beth Israel Hospital, Harvard Medical School, Boston, MA 02115;
Structural and Cell Biology Program, Department of Biomedical Sciences, Wadsworth Center, New York State Department of Health, Albany, NY 12201; and
¶ Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850
We have previously found that monkey V
2V
2+ T cells mount adaptive immune responses in response to Mycobacterium bovis bacillus Calmette-Guérin infections. We have now analyzed rhesus monkey T cell responses to nonpeptide Ags and superantigens. Like human V2V2+ T cells, rhesus monkey T cells are stimulated when exposed to prenyl pyrophosphate, bisphosphonate, and alkylamine Ags. Responsiveness was limited to T cells expressing V2V2 TCRs. Rhesus monkey V2V2+ T cells also responded to the superantigen, staphyloccocal enterotoxin A. Sequencing of the rhesus monkey V2V2 TCR revealed a strong sequence homology to human V2V2 TCR that preserves important sequence motifs. Moreover, chimeric TCRs that pair human V2 with monkey V2 and monkey V2 with human V2 retain reactivity to nonpeptide Ags and B cell lymphomas. A molecular model of the rhesus monkey V2V2 TCR has a basic region in the complementarity-determining region 3 binding groove that is similar to that seen in the human V2V2 TCR and preserves the topology of the complementarity-determining region loops. Thus, recognition of nonpeptide prenyl pyrophosphate, bisphosphonate, and alkylamine Ags is conserved in primates suggesting that primates can provide an animal model for human T cell Ag responses.
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