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* Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan;
Department of Biochemistry and
Institute for Dental Science, Matsumoto Dental University, Nagano, Japan;
Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan; and
¶ Department of Oral Microbiology, Kyushu Dental College, Fukuoka, Japan
Lipopolysaccharide is a pathogen that causes inflammatory bone loss. Monocytes and macrophages produce proinflammatory cytokines such as IL-1, TNF-
, and IL-6 in response to LPS. We examined the effects of LPS on the function of osteoclasts formed in vitro in comparison with its effect on bone marrow macrophages, osteoclast precursors. Both osteoclasts and bone marrow macrophages expressed mRNA of Toll-like receptor 4 (TLR4) and CD14, components of the LPS receptor system. LPS induced rapid degradation of I-
B in osteoclasts, and stimulated the survival of osteoclasts. LPS failed to support the survival of osteoclasts derived from C3H/HeJ mice, which possess a missense mutation in the TLR4 gene. The LPS-promoted survival of osteoclasts was not mediated by any of the cytokines known to prolong the survival of osteoclasts, such as IL-1
, TNF-, and receptor activator of NF-B ligand. LPS stimulated the production of proinflammatory cytokines such as IL-1, TNF-, and IL-6 in bone marrow macrophages and peritoneal macrophages, but not in osteoclasts. These results indicate that osteoclasts respond to LPS through TLR4, but the characteristics of osteoclasts are quite different from those of their precursors, macrophages, in terms of proinflammatory cytokine production in response to LPS.
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