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SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production ¹

Tomonori Ishii^2,3,, Kei Ohnuma^2,*, Akikazu Murakami, Naruhiko Takasawa, Tadanori Yamochi, Satoshi Iwata^*, Masahiko Uchiyama^*, Nam H. Dang, Hirotoshi Tanaka^* and Chikao Morimoto^4,*,

^* Division of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan; Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; and Department of Lymphoma/Myeloma, MD Anderson Cancer Center, Houston, TX 77030

An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjögren’s syndrome, systemic lupus erythematosus, and congenital heart block from anti-Ro52 Ab-positive mother. However, the physiological function of the autoantigen SS-A/Ro52 has not yet been elucidated. In this study, we describe the role of Ro52 protein in T cell activation. Overexpression of SS-A/Ro52 in Jurkat T cell resulted in enhanced IL-2 production following CD28 stimulation. Furthermore, transfection of anti-Ro52-specific small RNA duplexes partially blocked the expression of native and overexpressed Ro52 in Jurkat T cell, resulting in decreased IL-2 production via CD28 pathway in these cells. Finally, intracellular localization of Ro52 dramatically changed following CD28 stimulation. Our data reveal a novel function of Ro52 in CD28-mediated pathway, which eventually contributes to cytokine production and expression of the T cell biological programs.

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