The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gismondi, A.
Right arrow Articles by Santoni, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gismondi, A.
Right arrow Articles by Santoni, A.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*(L)-PROLINE
*L-TYROSINE
The Journal of Immunology, 2003, 170: 3065-3073.
Copyright © 2003 by The American Association of Immunologists

Proline-Rich Tyrosine Kinase 2 and Rac Activation by Chemokine and Integrin Receptors Controls NK Cell Transendothelial Migration1

Angela Gismondi2,*, Jordan Jacobelli*, Raffaele Strippoli*, Fabrizio Mainiero*, Alessandra Soriani*, Loredana Cifaldi*, Mario Piccoli*, Luigi Frati*,{dagger} and Angela Santoni*,{dagger}

* Department of Experimental Medicine and Pathology, Istituto Pasteur-Fondazione Cenci Bolognetti, University of Rome "La Sapienza," Rome, Italy; and {dagger} Mediterranean Institute of Neuroscience, Neuromed, Pozzilli, Italy

Protein tyrosine kinase activation is an important requisite for leukocyte migration. Herein we demonstrate that NK cell binding to endothelium activates proline-rich tyrosine kinase 2 (Pyk-2) and the small GTP binding protein Rac that are coupled to integrin and chemokine receptors. Chemokine-mediated, but not integrin-mediated, Pyk-2 and Rac activation was sensitive to pretreatment of NK cells with pertussis toxin, a pharmacological inhibitor of Gi protein-coupled receptors. Both Pyk-2 and Rac are functionally involved in chemokine-induced NK cell migration through endothelium or ICAM-1 or VCAM-1 adhesive proteins, as shown by the use of recombinant vaccinia viruses encoding dominant negative mutants of Pyk-2 and Rac. Moreover, we found that Pyk-2 is associated with the Rac guanine nucleotide exchange factor Vav, which undergoes tyrosine phosphorylation upon integrin triggering. Finally, we provide direct evidence for the involvement of Pyk-2 in the control of both chemokine- and integrin-mediated Rac activation. Collectively, our results indicate that Pyk-2 acts as a receptor-proximal link between integrin and chemokine receptor signaling, and the Pyk-2/Rac pathway plays a pivotal role in the control of NK cell transendothelial migration.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
D. R. Spurrell, N. A. Luckashenak, D. C. Minney, A. Chaplin, J. M. Penninger, R. S. Liwski, J. L. Clements, and K. A. West
Vav1 Regulates the Migration and Adhesion of Dendritic Cells
J. Immunol., July 1, 2009; 183(1): 310 - 318.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
X. Zhu, E. Boetticher, L. Wang, Y. Duan, J. Learoyd, and A. R. Leff
Proline-Rich Tyrosine Kinase 2 Regulates Spreading and Migration of Eosinophils after {beta}2-Integrin Adhesion
Am. J. Respir. Cell Mol. Biol., September 1, 2008; 39(3): 263 - 269.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Carlino, H. Stabile, S. Morrone, R. Bulla, A. Soriani, C. Agostinis, F. Bossi, C. Mocci, F. Sarazani, F. Tedesco, et al.
Recruitment of circulating NK cells through decidual tissues: a possible mechanism controlling NK cell accumulation in the uterus during early pregnancy
Blood, March 15, 2008; 111(6): 3108 - 3115.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. A. Hsia, S.-T. Lim, J. A. Bernard-Trifilo, S. K. Mitra, S. Tanaka, J. den Hertog, D. N. Streblow, D. Ilic, M. H. Ginsberg, and D. D. Schlaepfer
Integrin {alpha}4{beta}1 Promotes Focal Adhesion Kinase-Independent Cell Motility via {alpha}4 Cytoplasmic Domain-Specific Activation of c-Src
Mol. Cell. Biol., November 1, 2005; 25(21): 9700 - 9712.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
A. A. Maghazachi
Insights into Seven and Single Transmembrane-Spanning Domain Receptors and Their Signaling Pathways in Human Natural Killer Cells
Pharmacol. Rev., September 1, 2005; 57(3): 339 - 357.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. M. Melnychuk, P. Smith, C. N. Kreklywich, F. Ruchti, J. Vomaske, L. Hall, L. Loh, J. A. Nelson, S. L. Orloff, and D. N. Streblow
Mouse Cytomegalovirus M33 Is Necessary and Sufficient in Virus-Induced Vascular Smooth Muscle Cell Migration
J. Virol., August 15, 2005; 79(16): 10788 - 10795.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Zhao and G. M. Bokoch
Critical Role of Proline-Rich Tyrosine Kinase 2 in Reversion of the Adhesion-Mediated Suppression of Reactive Oxygen Species Generation by Human Neutrophils
J. Immunol., June 15, 2005; 174(12): 8049 - 8055.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Riol-Blanco, N. Sanchez-Sanchez, A. Torres, A. Tejedor, S. Narumiya, A. L. Corbi, P. Sanchez-Mateos, and J. L. Rodriguez-Fernandez
The Chemokine Receptor CCR7 Activates in Dendritic Cells Two Signaling Modules That Independently Regulate Chemotaxis and Migratory Speed
J. Immunol., April 1, 2005; 174(7): 4070 - 4080.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H.-C. Aasheim, J. Delabie, and E. F. Finne
Ephrin-A1 binding to CD4+ T lymphocytes stimulates migration and induces tyrosine phosphorylation of PYK2
Blood, April 1, 2005; 105(7): 2869 - 2876.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. F. Barber, M. Faure, and E. O. Long
LFA-1 Contributes an Early Signal for NK Cell Cytotoxicity
J. Immunol., September 15, 2004; 173(6): 3653 - 3659.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Rouhiainen, J. Kuja-Panula, E. Wilkman, J. Pakkanen, J. Stenfors, R. K. Tuominen, M. Lepantalo, O. Carpen, J. Parkkinen, and H. Rauvala
Regulation of monocyte migration by amphoterin (HMGB1)
Blood, August 15, 2004; 104(4): 1174 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Gismondi, L. Cifaldi, C. Mazza, S. Giliani, S. Parolini, S. Morrone, J. Jacobelli, E. Bandiera, L. Notarangelo, and A. Santoni
Impaired natural and CD16-mediated NK cell cytotoxicity in patients with WAS and XLT: ability of IL-2 to correct NK cell functional defect
Blood, July 15, 2004; 104(2): 436 - 443.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. E. Pontow, N. V. Heyden, S. Wei, and L. Ratner
Actin Cytoskeletal Reorganizations and Coreceptor-Mediated Activation of Rac during Human Immunodeficiency Virus-Induced Cell Fusion
J. Virol., July 1, 2004; 78(13): 7138 - 7147.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-S. Shi and J. H. Kehrl
Pyk2 Amplifies Epidermal Growth Factor and c-Src-induced Stat3 Activation
J. Biol. Chem., April 23, 2004; 279(17): 17224 - 17231.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. McLeod, A. J. Shum, R. L. Lee, F. Takei, and M. R. Gold
The Rap GTPases Regulate Integrin-mediated Adhesion, Cell Spreading, Actin Polymerization, and Pyk2 Tyrosine Phosphorylation in B Lymphocytes
J. Biol. Chem., March 26, 2004; 279(13): 12009 - 12019.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. K. Chandhoke, M. Williams, E. Schaefer, L. Zorn, and S. D. Blystone
{beta}3 integrin phosphorylation is essential for Arp3 organization into leukocyte {alpha}V{beta}3-vitronectin adhesion contacts
J. Cell Sci., March 15, 2004; 117(8): 1431 - 1441.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2003 by The American Association of Immunologists, Inc. All rights reserved.