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* Channing Laboratory, Department of Medicine,
Department of Neurology, and
Laboratory of Immunogenetics and Transplantation, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115; and
Department of Microbiology and Molecular Genetics, and
¶ School of Public Health, Department of Immunology and Infectious Disease, Harvard Medical School, Boston, MA 02115
Abscess formation associated with intra-abdominal sepsis causes severe morbidity and can be fatal. Previous studies have implicated T cells in the pathogenesis of abscess formation, and we have recently shown that CD4+ T cells activated in vitro by zwitterionic capsular polysaccharides from abscess-inducing bacteria such as Staphylococcus aureus and Bacteroides fragilis initiate this host response when transferred to naive rats. In this study, we show that mice deficient in 
TCR-bearing T cells or CD4+ T cells fail to develop abscesses following challenge with B. fragilis or abscess-inducing zwitterionic polysaccharides, compared with CD8-/- or wild-type animals. Transfer of CD4+ T cells from wild-type mice to TCR-/- animals reconstituted this ability. The induction of abscesses required T cell costimulation via the CD28-B7 pathway, and T cell transfer experiments with STAT4-/- and STAT6-/- mice demonstrated that this host response is dependent on STAT4 signaling. Significantly higher levels of IL-17, a proinflammatory cytokine produced almost exclusively by activated CD4+ T cells, were associated with abscess formation in Th2-impaired (STAT6-/-) mice, while STAT4-/- mice had significantly lower levels of this cytokine than control animals. The formation of abscesses was preceded by an increase in the number of activated CD4+ T cells in the peritoneal cavity 24 h following bacterial challenge. Confocal laser-scanning microscopy analysis revealed that CD4+ T cells comprise the abscess wall in these animals and produce IL-17 at this site. Administration of a neutralizing Ab specific for IL-17 prevented abscess formation following bacterial challenge in mice. These data delineate the specific T cell response necessary for the development of intra-abdominal abscesses and underscore the role of IL-17 in this disease process.
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