| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,¶
* Departments of Oral Biology and Microbiology, Immunobiology Vaccine Center, University of Alabama, Birmingham, AL 35294; Departments of
Mucosal Immunology and
Translation Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;
Department of Pathology, National Institute for Infectious Diseases, Tokyo, Japan;
¶ Division of Mucosal Immunology, Department of Microbiology and Immunology, Institute for Medical Sciences, University of Tokyo, Tokyo, Japan; and
|| Jissen Women’s College, Tokyo, Japan
Our previous studies showed that mucosal immunity was impaired in 1-year-old mice that had been orally immunized with OVA and native cholera toxin (nCT) as mucosal adjuvant. In this study, we queried whether similar immune dysregulation was also present in mucosal compartments of mice immunized by the nasal route. Both 1-year-old and young adult mice were immunized weekly with three nasal doses of OVA and nCT or with a nontoxic chimeric enterotoxin (mutant cholera toxin-A E112K/B subunit of native labile toxin) from Brevibacillus choshinensis. Elevated levels of OVA-specific IgG Abs in plasma and secretory IgA Abs in mucosal secretions (nasal washes, saliva, and fecal extracts) were noted in both young adult and 1-year-old mice given nCT or chimeric enterotoxin as mucosal adjuvants. Significant levels of OVA-specific CD4+ T cell proliferative and OVA-induced Th1- and Th2-type cytokine responses were noted in cervical lymph nodes and spleen of 1-year-old mice. In this regard, CD4+, CD45RB+ T cells were detected in greater numbers in the nasopharyngeal-associated lymphoreticular tissues of 1-year-old mice than of young adult mice, but the same did not hold true for Peyer’s patches or spleen. One-year-old mice given nasal tetanus toxoid plus the chimeric toxin as adjuvant were protected from lethal challenge with tetanus toxin. This result reinforced our findings that age-associated immune alterations occur first in gut-associated lymphoreticular tissues, and thus nasal delivery of vaccines for nasopharyngeal-associated lymphoreticular tissue-based mucosal immunity offers an attractive possibility to protect the elderly.
This article has been cited by other articles:
|
|
 |
|
  R. L. Schelonka, M. Zemlin, R. Kobayashi, G. C. Ippolito, Y. Zhuang, G. L. Gartland, A. Szalai, K. Fujihashi, K. Rajewsky, and H. W. Schroeder Jr. Preferential Use of DH Reading Frame 2 Alters B Cell Development and Antigen-Specific Antibody Production J. Immunol., December 15, 2008; 181(12): 8409 - 8415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sekine, K. Kataoka, Y. Fukuyama, Y. Adachi, J. Davydova, M. Yamamoto, R. Kobayashi, K. Fujihashi, H. Suzuki, D. T. Curiel, et al. A Novel Adenovirus Expressing Flt3 Ligand Enhances Mucosal Immunity by Inducing Mature Nasopharyngeal-Associated Lymphoreticular Tissue Dendritic Cell Migration J. Immunol., June 15, 2008; 180(12): 8126 - 8134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kataoka, K. Fujihashi, S. Sekine, T. Fukuiwa, R. Kobayashi, H. Suzuki, H. Nagata, K. Takatsu, S. Shizukuishi, J. R. McGhee, et al. Nasal Cholera Toxin Elicits IL-5 and IL-5 Receptor {alpha}-Chain Expressing B-1a B Cells for Innate Mucosal IgA Antibody Responses J. Immunol., May 15, 2007; 178(10): 6058 - 6065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kiyohara, T. Nagai, K. Munakata, K. Nonaka, T. Hanawa, S. J. Kim, and H. Yamada Stimulating Effect of Japanese Herbal (Kampo) Medicine, Hochuekkito on Upper Respiratory Mucosal Immune System Evid. Based Complement. Altern. Med., December 1, 2006; 3(4): 459 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hagiwara, Y. I. Kawamura, K. Kataoka, B. Rahima, R. J. Jackson, K. Komase, T. Dohi, P. N. Boyaka, Y. Takeda, H. Kiyono, et al. A Second Generation of Double Mutant Cholera Toxin Adjuvants: Enhanced Immunity without Intracellular Trafficking. J. Immunol., September 1, 2006; 177(5): 3045 - 3054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Ippolito, R. L. Schelonka, M. Zemlin, I. I. Ivanov, R. Kobayashi, C. Zemlin, G. L. Gartland, L. Nitschke, J. Pelkonen, K. Fujihashi, et al. Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production J. Exp. Med., June 12, 2006; 203(6): 1567 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kobayashi, T. Kohda, K. Kataoka, H. Ihara, S. Kozaki, D. W. Pascual, H. F. Staats, H. Kiyono, J. R. McGhee, and K. Fujihashi A Novel Neurotoxoid Vaccine Prevents Mucosal Botulism J. Immunol., February 15, 2005; 174(4): 2190 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Fayad, H. Zhang, D. Quinn, Y. Huang, and L. Qiao Oral Administration with Papillomavirus Pseudovirus Encoding IL-2 Fully Restores Mucosal and Systemic Immune Responses to Vaccinations in Aged Mice J. Immunol., August 15, 2004; 173(4): 2692 - 2698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kataoka, J. R. McGhee, R. Kobayashi, K. Fujihashi, S. Shizukuishi, and K. Fujihashi Nasal Flt3 Ligand cDNA Elicits CD11c+CD8+ Dendritic Cells for Enhanced Mucosal Immunity J. Immunol., March 15, 2004; 172(6): 3612 - 3619. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
