| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; and
Fudan University School of Medicine, Shanghai, China
Pertussis toxin (PTX) has been widely used as an adjuvant to induce Th1-mediated organ-specific autoimmune diseases in animal models. However, the cellular and molecular mechanisms remain to be defined. In this study, we showed that dendritic cells (DC) stimulated with PTX (PTX-DC) were able to substitute for PTX to promote experimental autoimmune uveitis (EAU). EAU induced by PTX-DC revealed a typical Th1 response, characterized by high uveitogenic retinal Ag interphotoreceptor retinoid-binding protein (IRBP)-specific IFN-
and IL-12 production in the draining lymph nodes, as well as increased levels of anti-IRBP IgG2a and decreased levels of anti-IRBP IgG1 in the serum of IRBP-immunized mice. Furthermore, PTX-DC preferentially induced T cells to produce the Th1 cytokine, IFN-. After being stimulated with PTX, DC exhibited up-regulation of MHC class II, CD80, CD86, CD40, and DEC205. PTX-DC had also increased allostimulatory capacity and IL-12 and TNF-
production. Serum IL-12 was increased in naive mice that received PTX-DC i.p. In addition, PTX activated extracellular signal-regulated kinase in DC. Following the inhibition of extracellular signal-regulated kinase signaling, the maturation of PTX-DC was reduced. Subsequently, the ability of PTX-DC to promote IFN- production by T cells in vitro and to induce EAU in vivo was blocked. The results suggest that PTX might exert an adjuvant effect on DC to promote their maturation and the production of proinflammatory cytokines, thereby eliciting a Th1 response.
Related articles in The JI:
This article has been cited by other articles:
|
|
 |
|
  Y. Allenbach, S. Solly, S. Gregoire, O. Dubourg, B. Salomon, G. Butler-Browne, L. Musset, S. Herson, D. Klatzmann, and O. Benveniste Role of Regulatory T Cells in a New Mouse Model of Experimental Autoimmune Myositis Am. J. Pathol., March 1, 2009; 174(3): 989 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Hou, H. S. Kang, and B. S. Kim Th17 cells enhance viral persistence and inhibit T cell cytotoxicity in a model of chronic virus infection J. Exp. Med., February 16, 2009; 206(2): 313 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Deng, M. Shi, M. Han, J. Zhong, Z. Li, W. Li, Y. Hu, L. Yan, J. Wang, Y. He, et al. Negative Regulation of Virus-triggered IFN-{beta} Signaling Pathway by Alternative Splicing of TBK1 J. Biol. Chem., December 19, 2008; 283(51): 35590 - 35597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Kel, B. Slutter, J. W. Drijfhout, F. Koning, and L. Nagelkerken Mannosylated self-peptide inhibits the development of experimental autoimmune encephalomyelitis via expansion of nonencephalitogenic T cells J. Leukoc. Biol., July 1, 2008; 84(1): 182 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, O. M. Z. Howard, and J. J. Oppenheim Pertussis Toxin by Inducing IL-6 Promotes the Generation of IL-17-Producing CD4 Cells J. Immunol., May 15, 2007; 178(10): 6123 - 6129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tang, W. Zhu, P. B. Silver, S.-B. Su, C.-C. Chan, and R. R. Caspi Autoimmune Uveitis Elicited with Antigen-Pulsed Dendritic Cells Has a Distinct Clinical Signature and Is Driven by Unique Effector Mechanisms: Initial Encounter with Autoantigen Defines Disease Phenotype J. Immunol., May 1, 2007; 178(9): 5578 - 5587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. M. Padigel, L. Stein, K. Redding, J. J. Lee, T. J. Nolan, G. A. Schad, L. Birnbaumer, and D. Abraham Signaling through G{alpha}i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice J. Leukoc. Biol., April 1, 2007; 81(4): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fujimoto, C.-R. Yu, G. Shi, B. P. Vistica, E. F. Wawrousek, D. M. Klinman, C.-C. Chan, C. E. Egwuagu, and I. Gery Pertussis Toxin Is Superior to TLR Ligands in Enhancing Pathogenic Autoimmunity, Targeted at a Neo-Self Antigen, by Triggering Robust Expansion of Th1 Cells and Their Cytokine Production J. Immunol., November 15, 2006; 177(10): 6896 - 6903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cassan, E. Piaggio, J. P. Zappulla, L. T. Mars, N. Couturier, F. Bucciarelli, S. Desbois, J. Bauer, D. Gonzalez-Dunia, and R. S. Liblau Pertussis Toxin Reduces the Number of Splenic Foxp3+ Regulatory T Cells J. Immunol., August 1, 2006; 177(3): 1552 - 1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, W. Hou, S. Sun, E. Bi, Y. Wang, M. Shi, J. Zang, C. Dong, and B. Sun Novel Function of IFN-{gamma}: Negative Regulation of Dendritic Cell Migration and T Cell Priming J. Immunol., July 15, 2006; 177(2): 934 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Fedele, P. Stefanelli, F. Spensieri, C. Fazio, P. Mastrantonio, and C. M. Ausiello Bordetella pertussis-Infected Human Monocyte-Derived Dendritic Cells Undergo Maturation and Induce Th1 Polarization and Interleukin-23 Expression Infect. Immun., March 1, 2005; 73(3): 1590 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, A. L. Carr, E. J. Donald, J. J. Skitzki, R. Okuyama, L. M. Stoolman, and A. E. Chang Synergistic Effects of IL-12 and IL-18 in Skewing Tumor-Reactive T-Cell Responses Towards a Type 1 Pattern Cancer Res., February 1, 2005; 65(3): 1063 - 1070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kerfoot, E. M. Long, M. J. Hickey, G. Andonegui, B. M. Lapointe, R. C. O. Zanardo, C. Bonder, W. G. James, S. M. Robbins, and P. Kubes TLR4 Contributes to Disease-Inducing Mechanisms Resulting in Central Nervous System Autoimmune Disease J. Immunol., December 1, 2004; 173(11): 7070 - 7077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Darabi, A. Y. Karulin, B. O. Boehm, H. H. Hofstetter, Z. Fabry, J. C. LaManna, J. C. Chavez, M. Tary-Lehmann, and P. V. Lehmann The Third Signal in T Cell-Mediated Autoimmune Disease? J. Immunol., July 1, 2004; 173(1): 92 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-I. Ham, S. J. Kim, J. Chen, B. P. Vistica, R. N. Fariss, R. S. Lee, E. F. Wawrousek, H. Takase, C.-R. Yu, C. E. Egwuagu, et al. Central Immunotolerance in Transgenic Mice Expressing a Foreign Antigen under Control of the Rhodopsin Promoter Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 857 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Teuscher, M. E. Poynter, H. Offner, A. Zamora, T. Watanabe, P. D. Fillmore, J. F. Zachary, and E. P. Blankenhorn Attenuation of Th1 Effector Cell Responses and Susceptibility to Experimental Allergic Encephalomyelitis in Histamine H2 Receptor Knockout Mice Is Due to Dysregulation of Cytokine Production by Antigen-Presenting Cells Am. J. Pathol., March 1, 2004; 164(3): 883 - 892. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
