| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
Activation of CD4+ Th cells requires their cognate interaction with APCs bearing specific relevant MHC class II-peptide complexes. This cognate interaction culminates in the formation of an immunological synapse that contains the various proteins and lipids required for efficient T cell activation. We now show that APC lipid raft membrane microdomains contain specific class II-peptide complexes and serve as platforms that deliver these raft-associated class II molecules to the immunological synapse. APC rafts are required for T cell:APC conjugate formation and T cell activation at low densities of relevant class II-peptide complexes, a requirement that can be overcome at high class II-peptide density. Analysis of confocal microscopy images revealed that over time APC lipid rafts, raft-associated relevant class II-peptide complexes, and even immunologically irrelevant class II molecules accumulate at the immunological synapse. As the immunological synapse matures, relevant class II-peptide complexes are sorted to a central region of the interface, while irrelevant class II molecules are excluded from this site. We propose that T cell activation is facilitated by recruitment of MHC class II-peptide complexes to the immunological synapse by virtue of their constitutive association with lipid raft microdomains.
This article has been cited by other articles:
|
|
 |
|
  R. Knorr, C. Karacsonyi, and R. Lindner Endocytosis of MHC molecules by distinct membrane rafts J. Cell Sci., May 15, 2009; 122(10): 1584 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gelin, I. Sloma, D. Charron, and N. Mooney Regulation of MHC II and CD1 antigen presentation: from ubiquity to security J. Leukoc. Biol., February 1, 2009; 85(2): 215 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Berger and P. A. Roche MHC class II transport at a glance J. Cell Sci., January 1, 2009; 122(1): 1 - 4. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Walseng, O. Bakke, and P. A. Roche Major Histocompatibility Complex Class II-Peptide Complexes Internalize Using a Clathrin- and Dynamin-independent Endocytosis Pathway J. Biol. Chem., May 23, 2008; 283(21): 14717 - 14727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Munoz, M. Mittelbrunn, H. de la Fuente, M. Perez-Martinez, A. Garcia-Perez, A. Ariza-Veguillas, F. Malavasi, M. Zubiaur, F. Sanchez-Madrid, and J. Sancho Antigen-induced clustering of surface CD38 and recruitment of intracellular CD38 to the immunologic synapse Blood, April 1, 2008; 111(7): 3653 - 3664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Van Komen, S. Mishra, J. Byrum, G. R. Chichili, J. C. Yaciuk, A. D. Farris, and W. Rodgers Early and Dynamic Polarization of T Cell Membrane Rafts and Constituents Prior to TCR Stop Signals J. Immunol., November 15, 2007; 179(10): 6845 - 6855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Szabo, A. Dolganiuc, Q. Dai, and S. B. Pruett TLR4, Ethanol, and Lipid Rafts: A New Mechanism of Ethanol Action with Implications for other Receptor-Mediated Effects J. Immunol., February 1, 2007; 178(3): 1243 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Unternaehrer, A. Chow, M. Pypaert, K. Inaba, and I. Mellman The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface PNAS, January 2, 2007; 104(1): 234 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Biro, L. Cervenak, A. Balogh, A. Lorincz, K. Uray, A. Horvath, L. Romics, J. Matko, G. Fust, and G. Laszlo Novel anti-cholesterol monoclonal immunoglobulin G antibodies as probes and potential modulators of membrane raft-dependent immune functions J. Lipid Res., January 1, 2007; 48(1): 19 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Schweitzer, A. M. Reding, H. M. Patton, T. P. Sullivan, C. E. Stubbs, E. Villalobos-Menuey, S. A. Huber, and M. K. Newell Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression J. Lipid Res., November 1, 2006; 47(11): 2525 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Poloso, L. K. Denzin, and P. A. Roche CDw78 Defines MHC Class II-Peptide Complexes That Require Ii Chain-Dependent Lysosomal Trafficking, Not Localization to a Specific Tetraspanin Membrane Microdomain J. Immunol., October 15, 2006; 177(8): 5451 - 5458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Karacsonyi, T. Bedke, N. Hinrichsen, R. Schwinzer, and R. Lindner MHC II molecules and invariant chain reside in membranes distinct from conventional lipid rafts J. Leukoc. Biol., November 1, 2005; 78(5): 1097 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Zilber, N. Setterblad, T. Vasselon, C. Doliger, D. Charron, N. Mooney, and C. Gelin MHC class II/CD38/CD9: a lipid-raft-dependent signaling complex in human monocytes Blood, November 1, 2005; 106(9): 3074 - 3081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Drenou, L. Amiot, N. Setterblad, S. Taque, V. Guilloux, D. Charron, R. Fauchet, and N. Mooney MHC class II signaling function is regulated during maturation of plasmacytoid dendritic cells J. Leukoc. Biol., April 1, 2005; 77(4): 560 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Nashar and J. R. Drake The Pathway of Antigen Uptake and Processing Dictates MHC Class II-Mediated B Cell Survival and Activation J. Immunol., February 1, 2005; 174(3): 1306 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O. Meyer zum Bueschenfelde, J. Unternaehrer, I. Mellman, and K. Bottomly Regulated Recruitment of MHC Class II and Costimulatory Molecules to Lipid Rafts in Dendritic Cells J. Immunol., November 15, 2004; 173(10): 6119 - 6124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Poloso, A. Muntasell, and P. A. Roche MHC Class II Molecules Traffic into Lipid Rafts during Intracellular Transport J. Immunol., October 1, 2004; 173(7): 4539 - 4546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Undale, P. J. van den Elsen, and E. Celis Antigen-independent acquisition of MHC class II molecules by human T lymphocytes Int. Immunol., October 1, 2004; 16(10): 1523 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Karacsonyi, R. Knorr, A. Fulbier, and R. Lindner Association of Major Histocompatibility Complex II with Cholesterol- and Sphingolipid-rich Membranes Precedes Peptide Loading J. Biol. Chem., August 13, 2004; 279(33): 34818 - 34826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Setterblad, S. Becart, D. Charron, and N. Mooney B Cell Lipid Rafts Regulate Both Peptide-Dependent and Peptide-Independent APC-T Cell Interaction J. Immunol., August 1, 2004; 173(3): 1876 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Sojka, D. Bruniquel, R. H. Schwartz, and N. J. Singh IL-2 Secretion by CD4+ T Cells In Vivo Is Rapid, Transient, and Influenced by TCR-Specific Competition J. Immunol., May 15, 2004; 172(10): 6136 - 6143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. El Fakhry, M. Bouillon, C. Leveille, A. Brunet, H. Khalil, J. Thibodeau, and W. Mourad Delineation of the HLA-DR Region and the Residues Involved in the Association with the Cytoskeleton J. Biol. Chem., April 30, 2004; 279(18): 18472 - 18480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gordy, S. Mishra, and W. Rodgers Visualization of Antigen Presentation by Actin-Mediated Targeting of Glycolipid-Enriched Membrane Domains to the Immune Synapse of B Cell APCs J. Immunol., February 15, 2004; 172(4): 2030 - 2038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Dolan, T. P. Phelan, D. Ilkovitch, L. Qi, W. F. Wade, T. M. Laufer, and S. Ostrand-Rosenberg Invariant Chain and the MHC Class II Cytoplasmic Domains Regulate Localization of MHC Class II Molecules to Lipid Rafts in Tumor Cell-Based Vaccines J. Immunol., January 15, 2004; 172(2): 907 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wong and L. C. Schlichter Differential Recruitment of Kv1.4 and Kv4.2 to Lipid Rafts by PSD-95 J. Biol. Chem., January 2, 2004; 279(1): 444 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Clatza, L. C. Bonifaz, D. A. A. Vignali, and J. Moreno CD40-Induced Aggregation of MHC Class II and CD80 on the Cell Surface Leads to an Early Enhancement in Antigen Presentation J. Immunol., December 15, 2003; 171(12): 6478 - 6487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Katzman and R. Longnecker Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus J. Gen. Virol., November 1, 2003; 84(11): 2987 - 2992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vereb, J. Szollosi, J. Matko, P. Nagy, T. Farkas, L. Vigh, L. Matyus, T. A. Waldmann, and S. Damjanovich Dynamic, yet structured: The cell membrane three decades after the Singer-Nicolson model PNAS, July 8, 2003; 100(14): 8053 - 8058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Setterblad, C. Roucard, C. Bocaccio, J.-P. Abastado, D. Charron, and N. Mooney Composition of MHC class II-enriched lipid microdomains is modified during maturation of primary dendritic cells J. Leukoc. Biol., July 1, 2003; 74(1): 40 - 48. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
