| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Cell Biology and Neuroscience, Rutgers, State University of New Jersey, Piscataway, NJ 08854
CD40 ligand (CD154) expression has been shown to be regulated, in
part, at the posttranscriptional level by a pathway of "regulated
instability" of mRNA decay throughout a time course of T cell
activation. This pathway is modulated at late times of
activation by the binding of a stability complex (termed complex I)
to a CU-rich region in the 3' untranslated region of the CD154
message. We have undertaken experiments to extend these findings and to
analyze the cis-acting elements and
trans-acting factors involved in this regulation. We
have previously shown that the minimal binding sequence for complex I
is a 63 nt CU-rich motif. However, our current study shows that when
this site was deleted additional complex binding was observed upstream
and downstream of the minimal binding region. Only after deletion of an
extended region (termed 
1515) was complex binding completely
abolished. Analysis of complex binding using competition experiments
revealed that the three adjacent regions bound related but not
identical complexes. However, all three sites appeared to have a 55-kDa
protein as the RNA-binding protein. Deletion of the 1515 region
resulted in reduced transcript stability as measured by both in vitro
and in vivo decay assays. Finally, using Abs against known RNA-binding
proteins, we identified the polypyrimidine tract-binding protein (or
heterogeneous nuclear ribonucleoprotein I) as a candidate
RNA-binding component of complex I.
Related articles in The JI:
This article has been cited by other articles:
|
|
 |
|
  B. J. Hamilton, X.-W. Wang, J. Collins, D. Bloch, A. Bergeron, B. Henry, B. M. Terry, M. Zan, A. J. Mouland, and W. F. C. Rigby Separate cis-trans Pathways Post-transcriptionally Regulate Murine CD154 (CD40 Ligand) Expression: A NOVEL FUNCTION FOR CA REPEATS IN THE 3'-UNTRANSLATED REGION J. Biol. Chem., September 12, 2008; 283(37): 25606 - 25616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Porter, S. Vavassori, and L. R. Covey A Polypyrimidine Tract-Binding Protein-Dependent Pathway of mRNA Stability Initiates with CpG Activation of Primary B Cells J. Immunol., September 1, 2008; 181(5): 3336 - 3345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X Li, V Rider, B F Kimler, and N I Abdou Estrogen does not regulate CD154 mRNA stability in systemic lupus erythematosus T cells Lupus, December 1, 2006; 15(12): 852 - 857. [Abstract] [PDF]
|
|
|
|
|
|
A. Pautz, K. Linker, T. Hubrich, R. Korhonen, S. Altenhofer, and H. Kleinert The Polypyrimidine Tract-binding Protein (PTB) Is Involved in the Post-transcriptional Regulation of Human Inducible Nitric Oxide Synthase Expression J. Biol. Chem., October 27, 2006; 281(43): 32294 - 32302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Singh, J. Laughlin, P. A. Kosinski, and L. R. Covey Nucleolin Is a Second Component of the CD154 mRNA Stability Complex That Regulates mRNA Turnover in Activated T Cells J. Immunol., July 15, 2004; 173(2): 976 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhang, C. J. Fichtenbaum, D. A. Hildeman, J. D. Lifson, and C. Chougnet CD40 Ligand Dysregulation in HIV Infection: HIV Glycoprotein 120 Inhibits Signaling Cascades Upstream of CD40 Ligand Transcription J. Immunol., February 15, 2004; 172(4): 2678 - 2686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chougnet Role of CD40 Ligand dysregulation in HIV-associated dysfunction of antigen-presenting cells J. Leukoc. Biol., November 1, 2003; 74(5): 702 - 709. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
