HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cai, D. Articles by Lerner, A. Search for Related Content PubMed PubMed Citation Articles by Cai, D. Articles by Lerner, A.

The GDP Exchange Factor AND-34 Is Expressed in B Cells, Associates With HEF1, and Activates Cdc42¹

Dongpo Cai^2,, Kyriakos N. Felekkis^2,, Richard I. Near^*, Geraldine M. O’Neill, Jean Maguire van Seventer, Erica A. Golemis and Adam Lerner^3,*,

^* Section of Hematology and Oncology and Department of Medicine, Boston Medical Center, Department of Pathology, Boston University School of Medicine, and Department of Environmental Health, Boston University School of Public Health, Boston, MA 02118; and Division of Basic Science, Fox Chase Cancer Center, Philadelphia, PA 19111

AND-34, a novel GDP exchange factor, is expressed constitutively at significant levels in murine splenic B cells, but not in murine splenic T cells or thymocytes. In B cell lines, anti-IgM treatment up-regulates AND-34 transcript levels. B cell AND-34 associates with both the docking molecules p130Cas and HEF1. AND-34 binds by its GDP exchange factor domain to the C terminus of HEF1, a region of HEF1 previously implicated in apoptotic, adhesion, and cell cycle-regulated signaling. Overexpression of AND-34 in murine B cell lines activates the Rho family GTPase Cdc42, but not Rac, Rho, RalA, or Rap1. Consistent with this, a subpopulation of AND-34 overexpressing B cells have long filamentous actin-containing cellular extensions. AND-34 overexpression augments both autophosphorylation and kinase activity of the Cdc42/Rac-responsive serine/threonine kinase PAK1. As previously reported for lymphoid cells transfected with constitutively active Cdc42, AND-34 overexpression inhibits SDF-1-induced B cell polarization. These studies suggest that p130Cas and HEF1-associated AND-34 may regulate B cell adhesion and motility through a Cdc42-mediated signaling pathway.

This article has been cited by other articles:

				G. M. O'Neill, S. Seo, I. G. Serebriiskii, S. R. Lessin, and E. A. Golemis A New Central Scaffold for Metastasis: Parsing HEF1/Cas-L/NEDD9 Cancer Res., October 1, 2007; 67(19): 8975 - 8979. [Abstract] [Full Text] [PDF]

				V. A. Rufanova and A. Sorokin CrkII Associates with BCAR3 in Response to Endothelin-1 in Human Glomerular Mesangial Cells. Experimental Biology and Medicine, June 1, 2006; 231(6): 752 - 756. [Abstract] [Full Text] [PDF]

				K. N. Felekkis, R. P. Narsimhan, R. Near, A. F. Castro, Y. Zheng, L. A. Quilliam, and A. Lerner AND-34 Activates Phosphatidylinositol 3-Kinase and Induces Anti-Estrogen Resistance in a SH2 and GDP Exchange Factor-Like Domain-Dependent Manner Mol. Cancer Res., January 1, 2005; 3(1): 32 - 41. [Abstract] [Full Text] [PDF]

				M. Dail, M. S. Kalo, J. A. Seddon, J.-F. Cote, K. Vuori, and E. B. Pasquale SHEP1 Function in Cell Migration Is Impaired by a Single Amino Acid Mutation That Disrupts Association with the Scaffolding Protein Cas but Not with Ras GTPases J. Biol. Chem., October 1, 2004; 279(40): 41892 - 41902. [Abstract] [Full Text] [PDF]